GWA Analysis for Milk Production Traits in Dairy Sheep and Genetic Support for a QTN Influencing Milk Protein Percentage in the LALBA Gene

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Standard

GWA Analysis for Milk Production Traits in Dairy Sheep and Genetic Support for a QTN Influencing Milk Protein Percentage in the LALBA Gene. / García-Gámez, Elsa; Gutiérrez-Gil, Beatriz; Sahana, Goutam; Sánchez, Juan-Pablo; Bayón, Yolanda; Arranz, Juan-José.

I: P L o S One, Bind 7, Nr. 10, 18.10.2012, s. e47782.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

García-Gámez, E, Gutiérrez-Gil, B, Sahana, G, Sánchez, J-P, Bayón, Y & Arranz, J-J 2012, 'GWA Analysis for Milk Production Traits in Dairy Sheep and Genetic Support for a QTN Influencing Milk Protein Percentage in the LALBA Gene', P L o S One, bind 7, nr. 10, s. e47782.

APA

García-Gámez, E., Gutiérrez-Gil, B., Sahana, G., Sánchez, J-P., Bayón, Y., & Arranz, J-J. (2012). GWA Analysis for Milk Production Traits in Dairy Sheep and Genetic Support for a QTN Influencing Milk Protein Percentage in the LALBA Gene. P L o S One, 7(10), e47782.

CBE

García-Gámez E, Gutiérrez-Gil B, Sahana G, Sánchez J-P, Bayón Y, Arranz J-J. 2012. GWA Analysis for Milk Production Traits in Dairy Sheep and Genetic Support for a QTN Influencing Milk Protein Percentage in the LALBA Gene. P L o S One. 7(10):e47782.

MLA

Vancouver

García-Gámez E, Gutiérrez-Gil B, Sahana G, Sánchez J-P, Bayón Y, Arranz J-J. GWA Analysis for Milk Production Traits in Dairy Sheep and Genetic Support for a QTN Influencing Milk Protein Percentage in the LALBA Gene. P L o S One. 2012 okt 18;7(10):e47782.

Author

García-Gámez, Elsa ; Gutiérrez-Gil, Beatriz ; Sahana, Goutam ; Sánchez, Juan-Pablo ; Bayón, Yolanda ; Arranz, Juan-José. / GWA Analysis for Milk Production Traits in Dairy Sheep and Genetic Support for a QTN Influencing Milk Protein Percentage in the LALBA Gene. I: P L o S One. 2012 ; Bind 7, Nr. 10. s. e47782.

Bibtex

@article{a691b4e47f7a4a5682f5b20c2de7bf9b,
title = "GWA Analysis for Milk Production Traits in Dairy Sheep and Genetic Support for a QTN Influencing Milk Protein Percentage in the LALBA Gene",
abstract = "In this study, we used the Illumina OvineSNP50 BeadChip to conduct a genome-wide association (GWA) analysis for milk production traits in dairy sheep by analyzing a commercial population of Spanish Churra sheep. The studied population consisted of a total of 1,681 Churra ewes belonging to 16 half-sib families with available records for milk yield (MY), milk protein and fat yields (PY and FY) and milk protein and fat contents (PP and FP). The most significant association identified reached experiment-wise significance for PP and FP and was located on chromosome 3 (OAR3). These results confirm thepopulation-level segregation of a previously reported QTL affecting PP and suggest that this QTL has a significant pleiotropic effect on FP. Further associations were detected at the chromosome-wise significance level on 14 other chromosomal regions. The marker on OAR3 showing the highest significant association was located at the third intron of the alpha-lactalbumin (LALBA) gene, which is a functional and positional candidate underlying this association. Sequencing this gene in the 16 Churra rams of the studied resource population identified additional polymorphisms. One out of the 31polymorphisms identified was located within the coding gene sequence (LALBA_g.242T.C) and was predicted to cause an amino acid change in the protein (Val27Ala). Different approaches, including GWA analysis, a combined linkage and linkage disequilibrium study and a concordance test with the QTL segregating status of the sires, were utilized to assess the role of this mutation as a putative QTN for the genetic effects detected on OAR3. Our results strongly support the polymorphism LALBA_g.242T.C as the most likely causal mutation of the studied OAR3 QTL affecting PP and FP, although we cannot ruleout the possibility that this SNP is in perfect linkage disequilibrium with the true causal polymorphism",
author = "Elsa Garc{\'i}a-G{\'a}mez and Beatriz Guti{\'e}rrez-Gil and Goutam Sahana and Juan-Pablo S{\'a}nchez and Yolanda Bay{\'o}n and Juan-Jos{\'e} Arranz",
year = "2012",
month = "10",
day = "18",
language = "English",
volume = "7",
pages = "e47782",
journal = "P L o S One",
issn = "1932-6203",
publisher = "public library of science",
number = "10",

}

RIS

TY - JOUR

T1 - GWA Analysis for Milk Production Traits in Dairy Sheep and Genetic Support for a QTN Influencing Milk Protein Percentage in the LALBA Gene

AU - García-Gámez, Elsa

AU - Gutiérrez-Gil, Beatriz

AU - Sahana, Goutam

AU - Sánchez, Juan-Pablo

AU - Bayón, Yolanda

AU - Arranz, Juan-José

PY - 2012/10/18

Y1 - 2012/10/18

N2 - In this study, we used the Illumina OvineSNP50 BeadChip to conduct a genome-wide association (GWA) analysis for milk production traits in dairy sheep by analyzing a commercial population of Spanish Churra sheep. The studied population consisted of a total of 1,681 Churra ewes belonging to 16 half-sib families with available records for milk yield (MY), milk protein and fat yields (PY and FY) and milk protein and fat contents (PP and FP). The most significant association identified reached experiment-wise significance for PP and FP and was located on chromosome 3 (OAR3). These results confirm thepopulation-level segregation of a previously reported QTL affecting PP and suggest that this QTL has a significant pleiotropic effect on FP. Further associations were detected at the chromosome-wise significance level on 14 other chromosomal regions. The marker on OAR3 showing the highest significant association was located at the third intron of the alpha-lactalbumin (LALBA) gene, which is a functional and positional candidate underlying this association. Sequencing this gene in the 16 Churra rams of the studied resource population identified additional polymorphisms. One out of the 31polymorphisms identified was located within the coding gene sequence (LALBA_g.242T.C) and was predicted to cause an amino acid change in the protein (Val27Ala). Different approaches, including GWA analysis, a combined linkage and linkage disequilibrium study and a concordance test with the QTL segregating status of the sires, were utilized to assess the role of this mutation as a putative QTN for the genetic effects detected on OAR3. Our results strongly support the polymorphism LALBA_g.242T.C as the most likely causal mutation of the studied OAR3 QTL affecting PP and FP, although we cannot ruleout the possibility that this SNP is in perfect linkage disequilibrium with the true causal polymorphism

AB - In this study, we used the Illumina OvineSNP50 BeadChip to conduct a genome-wide association (GWA) analysis for milk production traits in dairy sheep by analyzing a commercial population of Spanish Churra sheep. The studied population consisted of a total of 1,681 Churra ewes belonging to 16 half-sib families with available records for milk yield (MY), milk protein and fat yields (PY and FY) and milk protein and fat contents (PP and FP). The most significant association identified reached experiment-wise significance for PP and FP and was located on chromosome 3 (OAR3). These results confirm thepopulation-level segregation of a previously reported QTL affecting PP and suggest that this QTL has a significant pleiotropic effect on FP. Further associations were detected at the chromosome-wise significance level on 14 other chromosomal regions. The marker on OAR3 showing the highest significant association was located at the third intron of the alpha-lactalbumin (LALBA) gene, which is a functional and positional candidate underlying this association. Sequencing this gene in the 16 Churra rams of the studied resource population identified additional polymorphisms. One out of the 31polymorphisms identified was located within the coding gene sequence (LALBA_g.242T.C) and was predicted to cause an amino acid change in the protein (Val27Ala). Different approaches, including GWA analysis, a combined linkage and linkage disequilibrium study and a concordance test with the QTL segregating status of the sires, were utilized to assess the role of this mutation as a putative QTN for the genetic effects detected on OAR3. Our results strongly support the polymorphism LALBA_g.242T.C as the most likely causal mutation of the studied OAR3 QTL affecting PP and FP, although we cannot ruleout the possibility that this SNP is in perfect linkage disequilibrium with the true causal polymorphism

M3 - Journal article

VL - 7

SP - e47782

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 10

ER -