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Growth of and valine production by a Bacillus subtilis mutant in the small intestine of pigs

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Dietary supplementation with bacteria that are able to overproduce AA in the digestive tract and expected to have a probiotic effect could be a strategy to supply animals with AA and at the same time to gain benefits from the probiotic effect. Three diets were formulated: 1) a basal diet with a Val:Lys of 0.63:1 (Neg), 2) the Neg diet with added Bacillus subtilis-valine (1.28 × 108 cfu/g feed) (+Bac), and 3) the Neg diet with added L-Val to a Val:Lys of 0.69:1 (+Val). Eighteen gilts (6 on each treatment) with initial weights of ∼15 kg were fed the diets for 23 d before the animals were euthanized and samples from the small intestine were obtained. The number of B. subtilis cfu in digesta was higher in the +Bac group than in the Neg group (P < 0.001). In the epithelium, no B. subtilis cfu were detected in the Neg group, whereas numbers between 3.4 and 4.4 log cfu/g and numerically higher Val and Lys concentrations were measured in the +Bac group. Short-term in vitro incubations of digesta showed a decrease (P ≤ 0.03) in the number of B. subtilis cfu over time for the +Bac group and no difference in the rate of Val production compared to that in the Neg group. In conclusion, more B. subtilis cfu were present in the small intestinal digesta and epithelium of pigs fed the +Bac diet. However, the data indicated that the tested B. subtilis-valine was not able to grow in either digesta or in the epithelium and, thereby, to produce significant amounts of Val at these sites.
OriginalsprogEngelsk
TidsskriftJournal of Animal Science
Vol/bind94
Nummer7, supp. 3
Sider (fra-til)382-386
Antal sider5
ISSN0021-8812
DOI
StatusUdgivet - okt. 2016

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