Genetic linkage mapping in an F2 perennial ryegrass population using DArT markers

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  • Céline Tomaszewski , Teagasc, Crops Environment and Land Use Programme, Oak Park Research Centre, Irland
  • Stephen Byrne, Danmark
  • Alexandra Foito, Teagasc, Crops Environment and Land Use Programme, Oak Park Research Centre, Irland
  • Steven Kildea, Teagasc, Crops Environment and Land Use Programme, Oak Park Research Centre, Irland
  • David Kopecký , Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Tjekkiet
  • Jaroslaw Doležel , Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Tjekkiet
  • John Seymour (PAT) Heslop-Harrison, Department of Biology, University of Leicester, Storbritannien
  • D Stewart
  • ,
  • S Barth, Teagasc, Irland
Perennial ryegrass is the principal forage grass species used in temperate agriculture. In recent years, significant efforts have been made to develop molecular marker strategies to allow cost-effective characterization of a large number of loci simultaneously. One such strategy involves using DArT markers, and a DArT array has recently been developed for the Lolium-Festuca complex. In this study, we report the first use of the DArTFest array to generate a genetic linkage map based on 326 markers in a Lolium perenne F2 population, consisting of 325 genotypes. For proof of concept, the map was used to identify QTL associated with differences in crown rust susceptibility, caused by the fungal biotroph, Puccinia coronata.
OriginalsprogEngelsk
TidsskriftPlant Breeding
Vol/bind131
Nummer2
Sider (fra-til)345-349
Antal sider5
ISSN0179-9541
DOI
StatusUdgivet - apr. 2012

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