Generic Structures of Cytotoxic Liprotides: Nano-Sized Complexes with Oleic Acid Cores and Shells of Disordered Proteins

Jørn D Kaspersen, Jannik N Pedersen, Jon G Hansted, Søren B Nielsen, Srinivasan Sakthivel, Kristina Wilhelm, Ekaterina L Nemashkalova, Sergei E Permyakov, Eugene A Permyakov, Cristiano Luis Pinto Oliveira, Ludmilla A Morozova-Roche, Daniel Otzen, Jan Skov Pedersen

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Abstract

The cytotoxic complex formed between α-lactalbumin and oleic acid (OA) has inspired many studies on protein-fatty acid complexes, but structural insight remains sparse. After having used small-angle X-ray scattering (SAXS) to obtain structural information, we present a new, generic structural model of cytotoxic protein-oleic acid complexes, which we have termed liprotides (lipids and partially denatured proteins). Twelve liprotides formed from seven structurally unrelated proteins and prepared by different procedures all displayed core-shell structures, each with a micellar OA core and a shell consisting of flexible, partially unfolded protein, which stabilizes the OA micelle. The common structure explains similar effects exerted on cells by different liprotides and is consistent with a cargo off-loading of the OA into cell membranes.
OriginalsprogEngelsk
TidsskriftChemBioChem
Vol/bind10
Nummer18
Sider (fra-til)2693-2702
Antal sider10
ISSN1439-4227
DOI
StatusUdgivet - dec. 2014

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