Generic Structures of Cytotoxic Liprotides: Nano-Sized Complexes with Oleic Acid Cores and Shells of Disordered Proteins

Jørn D Kaspersen; Jannik N Pedersen; Jon G Hansted; Søren B Nielsen; Srinivasan Sakthivel; Kristina Wilhelm; Ekaterina L Nemashkalova; Sergei E Permyakov; Eugene A Permyakov; Cristiano Luis Pinto Oliveira; Ludmilla A Morozova-Roche; Daniel Otzen; Jan Skov Pedersen

doi:10.1002/cbic.201402407

Generic Structures of Cytotoxic Liprotides: Nano-Sized Complexes with Oleic Acid Cores and Shells of Disordered Proteins

Jørn D Kaspersen, Jannik N Pedersen, Jon G Hansted, Søren B Nielsen, Srinivasan Sakthivel, Kristina Wilhelm, Ekaterina L Nemashkalova, Sergei E Permyakov, Eugene A Permyakov, Cristiano Luis Pinto Oliveira, Ludmilla A Morozova-Roche, Daniel Otzen, Jan Skov Pedersen

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

38 Citationer (Scopus)

Abstract

The cytotoxic complex formed between α-lactalbumin and oleic acid (OA) has inspired many studies on protein-fatty acid complexes, but structural insight remains sparse. After having used small-angle X-ray scattering (SAXS) to obtain structural information, we present a new, generic structural model of cytotoxic protein-oleic acid complexes, which we have termed liprotides (lipids and partially denatured proteins). Twelve liprotides formed from seven structurally unrelated proteins and prepared by different procedures all displayed core-shell structures, each with a micellar OA core and a shell consisting of flexible, partially unfolded protein, which stabilizes the OA micelle. The common structure explains similar effects exerted on cells by different liprotides and is consistent with a cargo off-loading of the OA into cell membranes.

Originalsprog	Engelsk
Tidsskrift	ChemBioChem
Vol/bind	10
Nummer	18
Sider (fra-til)	2693-2702
Antal sider	10
ISSN	1439-4227
DOI	https://doi.org/10.1002/cbic.201402407
Status	Udgivet - dec. 2014

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10.1002/cbic.201402407

Citationsformater

Kaspersen, J. D., Pedersen, J. N., Hansted, J. G., Nielsen, S. B., Sakthivel, S., Wilhelm, K., Nemashkalova, E. L., Permyakov, S. E., Permyakov, E. A., Pinto Oliveira, C. L., Morozova-Roche, L. A., Otzen, D., & Pedersen, J. S. (2014). Generic Structures of Cytotoxic Liprotides: Nano-Sized Complexes with Oleic Acid Cores and Shells of Disordered Proteins. ChemBioChem, 10(18), 2693-2702. https://doi.org/10.1002/cbic.201402407

@article{f8f41922cdd0411c8a3af7d9d38ffd9a,

title = "Generic Structures of Cytotoxic Liprotides: Nano-Sized Complexes with Oleic Acid Cores and Shells of Disordered Proteins",

abstract = "The cytotoxic complex formed between α-lactalbumin and oleic acid (OA) has inspired many studies on protein-fatty acid complexes, but structural insight remains sparse. After having used small-angle X-ray scattering (SAXS) to obtain structural information, we present a new, generic structural model of cytotoxic protein-oleic acid complexes, which we have termed liprotides (lipids and partially denatured proteins). Twelve liprotides formed from seven structurally unrelated proteins and prepared by different procedures all displayed core-shell structures, each with a micellar OA core and a shell consisting of flexible, partially unfolded protein, which stabilizes the OA micelle. The common structure explains similar effects exerted on cells by different liprotides and is consistent with a cargo off-loading of the OA into cell membranes.",

author = "Kaspersen, {J{\o}rn D} and Pedersen, {Jannik N} and Hansted, {Jon G} and Nielsen, {S{\o}ren B} and Srinivasan Sakthivel and Kristina Wilhelm and Nemashkalova, {Ekaterina L} and Permyakov, {Sergei E} and Permyakov, {Eugene A} and {Pinto Oliveira}, {Cristiano Luis} and Morozova-Roche, {Ludmilla A} and Daniel Otzen and Pedersen, {Jan Skov}",

note = "{\textcopyright} 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",

year = "2014",

month = dec,

doi = "10.1002/cbic.201402407",

language = "English",

volume = "10",

pages = "2693--2702",

journal = "ChemBioChem",

issn = "1439-4227",

publisher = "John Wiley & Sons Inc.",

number = "18",

}

Kaspersen, JD, Pedersen, JN, Hansted, JG, Nielsen, SB, Sakthivel, S, Wilhelm, K, Nemashkalova, EL, Permyakov, SE, Permyakov, EA, Pinto Oliveira, CL, Morozova-Roche, LA, Otzen, D & Pedersen, JS 2014, 'Generic Structures of Cytotoxic Liprotides: Nano-Sized Complexes with Oleic Acid Cores and Shells of Disordered Proteins', ChemBioChem, bind 10, nr. 18, s. 2693-2702. https://doi.org/10.1002/cbic.201402407

Generic Structures of Cytotoxic Liprotides: Nano-Sized Complexes with Oleic Acid Cores and Shells of Disordered Proteins. / Kaspersen, Jørn D; Pedersen, Jannik N; Hansted, Jon G et al.
I: ChemBioChem, Bind 10, Nr. 18, 12.2014, s. 2693-2702.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - Generic Structures of Cytotoxic Liprotides

T2 - Nano-Sized Complexes with Oleic Acid Cores and Shells of Disordered Proteins

AU - Kaspersen, Jørn D

AU - Pedersen, Jannik N

AU - Hansted, Jon G

AU - Nielsen, Søren B

AU - Sakthivel, Srinivasan

AU - Wilhelm, Kristina

AU - Nemashkalova, Ekaterina L

AU - Permyakov, Sergei E

AU - Permyakov, Eugene A

AU - Pinto Oliveira, Cristiano Luis

AU - Morozova-Roche, Ludmilla A

AU - Otzen, Daniel

AU - Pedersen, Jan Skov

PY - 2014/12

Y1 - 2014/12

N2 - The cytotoxic complex formed between α-lactalbumin and oleic acid (OA) has inspired many studies on protein-fatty acid complexes, but structural insight remains sparse. After having used small-angle X-ray scattering (SAXS) to obtain structural information, we present a new, generic structural model of cytotoxic protein-oleic acid complexes, which we have termed liprotides (lipids and partially denatured proteins). Twelve liprotides formed from seven structurally unrelated proteins and prepared by different procedures all displayed core-shell structures, each with a micellar OA core and a shell consisting of flexible, partially unfolded protein, which stabilizes the OA micelle. The common structure explains similar effects exerted on cells by different liprotides and is consistent with a cargo off-loading of the OA into cell membranes.

AB - The cytotoxic complex formed between α-lactalbumin and oleic acid (OA) has inspired many studies on protein-fatty acid complexes, but structural insight remains sparse. After having used small-angle X-ray scattering (SAXS) to obtain structural information, we present a new, generic structural model of cytotoxic protein-oleic acid complexes, which we have termed liprotides (lipids and partially denatured proteins). Twelve liprotides formed from seven structurally unrelated proteins and prepared by different procedures all displayed core-shell structures, each with a micellar OA core and a shell consisting of flexible, partially unfolded protein, which stabilizes the OA micelle. The common structure explains similar effects exerted on cells by different liprotides and is consistent with a cargo off-loading of the OA into cell membranes.

U2 - 10.1002/cbic.201402407

DO - 10.1002/cbic.201402407

M3 - Journal article

C2 - 25403886

SN - 1439-4227

VL - 10

SP - 2693

EP - 2702

JO - ChemBioChem

JF - ChemBioChem

IS - 18

ER -

Generic Structures of Cytotoxic Liprotides: Nano-Sized Complexes with Oleic Acid Cores and Shells of Disordered Proteins

Abstract

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