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Generation of Fam83h knockout mice by CRISPR/Cas9-mediated gene engineering

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DOI

  • Sherko Nasseri, Kurdistan University of Medical Sciences
  • ,
  • Bahram Nikkho, Kurdistan University of Medical Sciences
  • ,
  • Sara Parsa, Kurdistan University of Medical Sciences
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  • Asghar Ebadifar, Shahid Beheshti University of Medical Sciences
  • ,
  • Farzad Soleimani, Faculty of Chemistry, University of Tabriz
  • ,
  • Karim Rahimi
  • Zakaria Vahabzadeh, Kurdistan University of Medical Sciences
  • ,
  • Mohammad Bagher Khadem-Erfan, Kurdistan University of Medical Sciences
  • ,
  • Jalal Rostamzadeh, University of Kurdistan
  • ,
  • Babak Baban, Augusta University
  • ,
  • Omid Banafshi, Kurdistan University of Medical Sciences
  • ,
  • Vahideh Assadollahi, Kurdistan University of Medical Sciences
  • ,
  • Sako Mirzaie, Islamic Azad University, Sanandaj Branch
  • ,
  • Fardin Fathi

Family with sequence similarity 83 member H (FAM83H) protein-coding geneplay an essential role in the structural organization, calcification of developing enamel, and keratin cytoskeleton disassembly by recruiting Casein kinase 1 alpha (CSNK1A1) to keratin filaments. In this study, we have applied CRISPR Cas9 nickase (D10A) to knockout (KO) the Fam83h gene in NMRI outbred mice. We generated homozygous Fam83h KO mice (Fam83h Ko/Ko) through a premature termination codon, which was validated by Sanger sequencing in F0 generation. Next, we also bred the FAM83H KO for two generations. Reverse-transcription polymerase chain reaction and Western blot analysis approved the Fam83h KO mice. The Fam83h KO mice had evidence of normal morphology at the cervical loops, secretory and maturation stages, and mandibular molars. In comparison with the normal wild-type mice (Fam83h W/W), the F2 homozygous KO (Fam83h Ko/Ko) had sparse, scruffy coats with small body size and decreased general activity. Also, they had the natural reproductive ability and natural lifespan. In addition, delay in opening the eyes and dry eyes among infant mice were seen. The F1 heterozygous mice looked comparable to the normal wild-type mice (Fam83h W/W), which showed autosomal recessive inheritance of these phenotypes. The KO of FAM83H had controversial effects on the development of teeth and the formation of enamel. The phenotype defect in dental development and the enamel formation were seen in three mice among four generations. It can be concluded that null FAM83H in outbred mice not only showed the reported phenotypes in null inbred mouse but also showed normal lifespan and reproductive ability; dental deficiency in three homozygous mice; and the symptoms that were similar to the symptoms of dry eye syndrome and curly coat dog syndrome in all four evaluated KO generations.

OriginalsprogEngelsk
BogserieJournal of Cellular Biochemistry
ISSN0730-2312
DOI
StatusE-pub ahead of print - 3 feb. 2019

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