Generation of an induced pluripotent stem cell line (DANi-011A) from a Parkinson's disease patient with a LRRK2 p.G2019S mutation

Muwan Chen; Muyesier Maimaitili; Susanne Hvolbøl Buchholdt; Uffe Birk Jensen; Fabia Febbraro; Mark Denham

doi:10.1016/j.scr.2020.101781

Generation of an induced pluripotent stem cell line (DANi-011A) from a Parkinson's disease patient with a LRRK2 p.G2019S mutation

Muwan Chen, Muyesier Maimaitili, Susanne Hvolbøl Buchholdt, Uffe Birk Jensen, Fabia Febbraro, Mark Denham^*

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

1 Citationer (Scopus)

Abstract

We generated an induced pluripotent stem cell (iPSC) line from fibroblasts of a clinically diagnosed 70 year old female Parkinson's disease (PD) patient heterozygous for a pathogenic missense variant (p.G2019S; c. 6055 G > A) in the leucine-rich repeat kinase 2 (LRRK2) gene by using non-integrating Sendai viruses. The DANi-011A iPSC line has a normal karyotype and is free from Sendai viruses. The expression of pluripotent markers in the iPSC line was confirmed by immunofluorescent staining, and we confirmed its ability to differentiate into the three germ layers. The DANi-011A iPSC line can be used for modeling PD and as a drug-screening platform.

Originalsprog	Engelsk
Artikelnummer	101781
Tidsskrift	Stem Cell Research
Vol/bind	45
ISSN	1873-5061
DOI	https://doi.org/10.1016/j.scr.2020.101781
Status	Udgivet - maj 2020

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title = "Generation of an induced pluripotent stem cell line (DANi-011A) from a Parkinson's disease patient with a LRRK2 p.G2019S mutation",

abstract = "We generated an induced pluripotent stem cell (iPSC) line from fibroblasts of a clinically diagnosed 70 year old female Parkinson's disease (PD) patient heterozygous for a pathogenic missense variant (p.G2019S; c. 6055 G > A) in the leucine-rich repeat kinase 2 (LRRK2) gene by using non-integrating Sendai viruses. The DANi-011A iPSC line has a normal karyotype and is free from Sendai viruses. The expression of pluripotent markers in the iPSC line was confirmed by immunofluorescent staining, and we confirmed its ability to differentiate into the three germ layers. The DANi-011A iPSC line can be used for modeling PD and as a drug-screening platform.",

author = "Muwan Chen and Muyesier Maimaitili and Buchholdt, {Susanne Hvolb{\o}l} and Jensen, {Uffe Birk} and Fabia Febbraro and Mark Denham",

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doi = "10.1016/j.scr.2020.101781",

language = "English",

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journal = "Stem Cell Research",

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Generation of an induced pluripotent stem cell line (DANi-011A) from a Parkinson's disease patient with a LRRK2 p.G2019S mutation. / Chen, Muwan ; Maimaitili, Muyesier ; Buchholdt, Susanne Hvolbøl et al.
I: Stem Cell Research, Bind 45, 101781, 05.2020.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - Generation of an induced pluripotent stem cell line (DANi-011A) from a Parkinson's disease patient with a LRRK2 p.G2019S mutation

AU - Chen, Muwan

AU - Maimaitili, Muyesier

AU - Buchholdt, Susanne Hvolbøl

AU - Jensen, Uffe Birk

AU - Febbraro, Fabia

AU - Denham, Mark

PY - 2020/5

Y1 - 2020/5

N2 - We generated an induced pluripotent stem cell (iPSC) line from fibroblasts of a clinically diagnosed 70 year old female Parkinson's disease (PD) patient heterozygous for a pathogenic missense variant (p.G2019S; c. 6055 G > A) in the leucine-rich repeat kinase 2 (LRRK2) gene by using non-integrating Sendai viruses. The DANi-011A iPSC line has a normal karyotype and is free from Sendai viruses. The expression of pluripotent markers in the iPSC line was confirmed by immunofluorescent staining, and we confirmed its ability to differentiate into the three germ layers. The DANi-011A iPSC line can be used for modeling PD and as a drug-screening platform.

AB - We generated an induced pluripotent stem cell (iPSC) line from fibroblasts of a clinically diagnosed 70 year old female Parkinson's disease (PD) patient heterozygous for a pathogenic missense variant (p.G2019S; c. 6055 G > A) in the leucine-rich repeat kinase 2 (LRRK2) gene by using non-integrating Sendai viruses. The DANi-011A iPSC line has a normal karyotype and is free from Sendai viruses. The expression of pluripotent markers in the iPSC line was confirmed by immunofluorescent staining, and we confirmed its ability to differentiate into the three germ layers. The DANi-011A iPSC line can be used for modeling PD and as a drug-screening platform.

UR - http://www.scopus.com/inward/record.url?scp=85083109266&partnerID=8YFLogxK

U2 - 10.1016/j.scr.2020.101781

DO - 10.1016/j.scr.2020.101781

M3 - Journal article

C2 - 32305865

AN - SCOPUS:85083109266

SN - 1873-5061

VL - 45

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 101781

ER -

Generation of an induced pluripotent stem cell line (DANi-011A) from a Parkinson's disease patient with a LRRK2 p.G2019S mutation

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