GENEPEASE Genomic tools for assessment of pesticide effects on the agricultural soil ecosystem

Carsten Suhr Jacobsen; Louise Feld; Mathis Hjort Hjelmsø; Morten Schostag Nielsen; Regin Rønn; Flemming Ekelund; Anders Johnsen; J G Sørensen; Paul Henning Krogh

GENEPEASE Genomic tools for assessment of pesticide effects on the agricultural soil ecosystem

Carsten Suhr Jacobsen, Louise Feld, Mathis Hjort Hjelmsø, Morten Schostag Nielsen, Regin Rønn, Flemming Ekelund, Anders Johnsen, J G Sørensen, Paul Henning Krogh

Publikation: Working paper/Preprint › Working paper › Forskning › peer review

Abstract

The project focussed on validating RNA based methods as potential genomic tools in assessment of agricultural soil ecosystems. It was shown that the mRNA based technique was very sensitive and the effects was seen in the same situations as when the OECD nitrification assay showed an effect. 16S rRNA based pyrosequencing of bacterial communities in soil was shown to report different than just DNA based analysis and indicated unlike the DNA measurement that the community was developing. Finally microarray analysis was compared to traditional test for toxicity testing of Folsomia candida and showed a more sensitive response to the pesticide exposure.

Originalsprog	Engelsk
Udgiver	Bekæmpelsesmiddelforskning fra Miljøstyrelsen
Sider	1-53
Antal sider	53
ISBN (Trykt)	978-87-93283-27-5
Status	Udgivet - 23 aug. 2016

Andre filer og links

http://www2.mst.dk/Udgiv/publications/2016/06/978-87-93283-27-5.pdf

GENEPEASE: GENEPEASE: GENomic tools for assEssment of Pesticide Effects on the Agricultural Soil Ecosystem
Krogh, P. H. (Deltager), Sørensen, J. G. (Deltager) & Roelofs, D. (Deltager)
01/03/2011 → 01/03/2013
Projekter: Projekt › Forskning

Citationsformater

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AU - Jacobsen, Carsten Suhr

AU - Feld, Louise

AU - Hjelmsø, Mathis Hjort

AU - Nielsen, Morten Schostag

AU - Rønn, Regin

AU - Ekelund, Flemming

AU - Johnsen, Anders

AU - Sørensen, J G

AU - Krogh, Paul Henning

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N2 - The project focussed on validating RNA based methods as potential genomic tools in assessment of agricultural soil ecosystems. It was shown that the mRNA based technique was very sensitive and the effects was seen in the same situations as when the OECD nitrification assay showed an effect. 16S rRNA based pyrosequencing of bacterial communities in soil was shown to report different than just DNA based analysis and indicated unlike the DNA measurement that the community was developing. Finally microarray analysis was compared to traditional test for toxicity testing of Folsomia candida and showed a more sensitive response to the pesticide exposure.

AB - The project focussed on validating RNA based methods as potential genomic tools in assessment of agricultural soil ecosystems. It was shown that the mRNA based technique was very sensitive and the effects was seen in the same situations as when the OECD nitrification assay showed an effect. 16S rRNA based pyrosequencing of bacterial communities in soil was shown to report different than just DNA based analysis and indicated unlike the DNA measurement that the community was developing. Finally microarray analysis was compared to traditional test for toxicity testing of Folsomia candida and showed a more sensitive response to the pesticide exposure.

UR - http://www2.mst.dk/Udgiv/publications/2016/06/978-87-93283-27-5.pdf

M3 - Working paper

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BT - GENEPEASE Genomic tools for assessment of pesticide effects on the agricultural soil ecosystem

PB - Bekæmpelsesmiddelforskning fra Miljøstyrelsen

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GENEPEASE Genomic tools for assessment of pesticide effects on the agricultural soil ecosystem

Abstract

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Fingeraftryk

Projekter

GENEPEASE: GENEPEASE: GENomic tools for assEssment of Pesticide Effects on the Agricultural Soil Ecosystem

Citationsformater