Gene-tracking of in vitro differentiation capabilities of adult mouse neural stem/progenitor cells

K. Ludlow, P. Kumar, G. Simpson, J. Buttigieg*

*Corresponding author af dette arbejde

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Previous studies have suggested that neurotransmitters may play an important role in influencing neural stem/progenitor cells (NSPCs) differentiation into oligodendrocytes (OL). Differentiated OL are known to aid in remyelination and axonal protection. In order to derive pure lineages of OL, especially for use in regenerative medicine, improved differentiation protocols are required. One potential differ-rentiation cue is the excitatory neurotransmitter glutamate, but, its effect on NSPCs is currently unknown. Using real-time PCR, we examined the in vitro ability of glutamate, in combination of growth factors to influence the NSPCs differentiation. Glutamate (200μM) for 14-days did not induce significant changes in the gene expression profile of differentiated cultures. Moreover, the combination of growth factors (FGF2, PDGF, NT3) demonstrated no significant effect on the proportions of differentiated cell types. Although the differentiation media, containing fetal bovine serum, had an effect on appearance of neuronal lineage, the overall expression of mature cell markers continued to decrease by day-14. In contrast, Matrigel-attached cultures, demonstrated significant increase in glial markers, with possible inhibition of neuronal differentiation as observed with spontaneous attachment. Overall, these data suggest that glutamate (200μM) and combination of growth factors had no modulating effect on the gene expression profile of differentiated NSPCs culture.

TidsskriftJournal of Stem Cells
Sider (fra-til)67-82
Antal sider16
StatusUdgivet - 2018


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