TY - JOUR
T1 - Gas Chromatography-Tandem Mass Spectrometry Method for Analyses of Iodoform and Diiodomethane in Multiple Biological Matrices from Dairy Cattle
AU - Rønn, Marie
AU - Nielsen, Mette Olaf
AU - Nørskov, Natalja P.
N1 - Publisher Copyright:
© 2024 The Authors. Published by American Chemical Society.
PY - 2025/1/8
Y1 - 2025/1/8
N2 - A quantitative method was developed and validated to analyze iodoform and its potential metabolite, diiodomethane, in biological fluids from dairy cows, including rumen fluid, duodenal fluid, blood serum, milk, and urine, using liquid-liquid extraction (LLE) and GC-MS/MS. The method showed no matrix effects across different samples, recoveries of spiked samples between 70 and 120%, and relative standard deviations (RSD%) ranging from 0.7 to 14%. Inter- and intravariations in quality control samples were within 3.1-12.3%, with the highest variation noted in iodoform spiking. Normalized internal standard (IS) and matrix factor (MF) calculations confirmed the suitability of diiodomethane-d2 as an internal standard for quantifying both compounds across different matrices. These results validate the method’s application for ADME (absorption, distribution, metabolism, and excretion) studies, enabling the investigation of iodoform digestion, metabolism, and excretion in dairy cattle.
AB - A quantitative method was developed and validated to analyze iodoform and its potential metabolite, diiodomethane, in biological fluids from dairy cows, including rumen fluid, duodenal fluid, blood serum, milk, and urine, using liquid-liquid extraction (LLE) and GC-MS/MS. The method showed no matrix effects across different samples, recoveries of spiked samples between 70 and 120%, and relative standard deviations (RSD%) ranging from 0.7 to 14%. Inter- and intravariations in quality control samples were within 3.1-12.3%, with the highest variation noted in iodoform spiking. Normalized internal standard (IS) and matrix factor (MF) calculations confirmed the suitability of diiodomethane-d2 as an internal standard for quantifying both compounds across different matrices. These results validate the method’s application for ADME (absorption, distribution, metabolism, and excretion) studies, enabling the investigation of iodoform digestion, metabolism, and excretion in dairy cattle.
KW - biological samples
KW - gas chromatography
KW - iodoform and diiodomethane quantification
KW - liquid−liquid extraction
KW - method development
UR - http://www.scopus.com/inward/record.url?scp=85212547105&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.4c08419
DO - 10.1021/acs.jafc.4c08419
M3 - Journal article
C2 - 39694665
AN - SCOPUS:85212547105
SN - 0021-8561
VL - 73
SP - 873
EP - 879
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 1
ER -