Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics

A. P. Carter; W. M. Clemons, Jr.; Ditlev Egeskov Brodersen; R. J. Morgan-Warren; B. T. Wimberly; V. Ramakrishnan

Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics

A. P. Carter, W. M. Clemons, Jr., Ditlev Egeskov Brodersen, R. J. Morgan-Warren, B. T. Wimberly, V. Ramakrishnan

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

1355 Citationer (Scopus)

Abstract

The 30S ribosomal subunit has two primary functions in protein synthesis. It discriminates against aminoacyl transfer RNAs that do not match the codon of messenger RNA, thereby ensuring accuracy in translation of the genetic message in a process called decoding. Also, it works with the 50S subunit to move the tRNAs and associated mRNA by precisely one codon, in a process called translocation. Here we describe the functional implications of the high-resolution 30S crystal structure presented in the accompanying paper, and infer details of the interactions between the 30S subunit and its tRNA and mRNA ligands. We also describe the crystal structure of the 30S subunit complexed with the antibiotics paromomycin, streptomycin and spectinomycin, which interfere with decoding and translocation. This work reveals the structural basis for the action of these antibiotics, and leads to a model for the role of the universally conserved 16S RNA residues A1492 and A1493 in the decoding process.

Originalsprog	Engelsk
Tidsskrift	Nature
Vol/bind	407
Sider (fra-til)	340-348
ISSN	0028-0836
Status	Udgivet - 2000
Udgivet eksternt	Ja

Citationsformater

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abstract = "The 30S ribosomal subunit has two primary functions in protein synthesis. It discriminates against aminoacyl transfer RNAs that do not match the codon of messenger RNA, thereby ensuring accuracy in translation of the genetic message in a process called decoding. Also, it works with the 50S subunit to move the tRNAs and associated mRNA by precisely one codon, in a process called translocation. Here we describe the functional implications of the high-resolution 30S crystal structure presented in the accompanying paper, and infer details of the interactions between the 30S subunit and its tRNA and mRNA ligands. We also describe the crystal structure of the 30S subunit complexed with the antibiotics paromomycin, streptomycin and spectinomycin, which interfere with decoding and translocation. This work reveals the structural basis for the action of these antibiotics, and leads to a model for the role of the universally conserved 16S RNA residues A1492 and A1493 in the decoding process.",

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T1 - Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics

AU - Carter, A. P.

AU - Clemons, Jr., W. M.

AU - Brodersen, Ditlev Egeskov

AU - Morgan-Warren, R. J.

AU - Wimberly, B. T.

AU - Ramakrishnan, V.

PY - 2000

Y1 - 2000

N2 - The 30S ribosomal subunit has two primary functions in protein synthesis. It discriminates against aminoacyl transfer RNAs that do not match the codon of messenger RNA, thereby ensuring accuracy in translation of the genetic message in a process called decoding. Also, it works with the 50S subunit to move the tRNAs and associated mRNA by precisely one codon, in a process called translocation. Here we describe the functional implications of the high-resolution 30S crystal structure presented in the accompanying paper, and infer details of the interactions between the 30S subunit and its tRNA and mRNA ligands. We also describe the crystal structure of the 30S subunit complexed with the antibiotics paromomycin, streptomycin and spectinomycin, which interfere with decoding and translocation. This work reveals the structural basis for the action of these antibiotics, and leads to a model for the role of the universally conserved 16S RNA residues A1492 and A1493 in the decoding process.

AB - The 30S ribosomal subunit has two primary functions in protein synthesis. It discriminates against aminoacyl transfer RNAs that do not match the codon of messenger RNA, thereby ensuring accuracy in translation of the genetic message in a process called decoding. Also, it works with the 50S subunit to move the tRNAs and associated mRNA by precisely one codon, in a process called translocation. Here we describe the functional implications of the high-resolution 30S crystal structure presented in the accompanying paper, and infer details of the interactions between the 30S subunit and its tRNA and mRNA ligands. We also describe the crystal structure of the 30S subunit complexed with the antibiotics paromomycin, streptomycin and spectinomycin, which interfere with decoding and translocation. This work reveals the structural basis for the action of these antibiotics, and leads to a model for the role of the universally conserved 16S RNA residues A1492 and A1493 in the decoding process.

M3 - Journal article

SN - 0028-0836

VL - 407

SP - 340

EP - 348

JO - Nature

JF - Nature

ER -

Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics

Abstract

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Citationsformater