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Functional in vitro screening of probiotic strains for inoculation of piglets as a prophylactic measure towards Enterotoxigenic Escherichia coli infection

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Functional in vitro screening of probiotic strains for inoculation of piglets as a prophylactic measure towards Enterotoxigenic Escherichia coli infection. / Hansen, Lea Hübertz Birch; Nielsen, B; Boll, E J; Skjøt-Rasmussen, L; Wellejus, A; Jørgensen, L ; Lauridsen, Charlotte; Canibe, Nuria.

I: Journal of Microbiological Methods, Bind 180, 106126, 01.2021.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

APA

Hansen, L. H. B., Nielsen, B., Boll, E. J., Skjøt-Rasmussen, L., Wellejus, A., Jørgensen, L., Lauridsen, C., & Canibe, N. (2021). Functional in vitro screening of probiotic strains for inoculation of piglets as a prophylactic measure towards Enterotoxigenic Escherichia coli infection. Journal of Microbiological Methods, 180, [106126]. https://doi.org/10.1016/j.mimet.2020.106126

CBE

MLA

Vancouver

Hansen LHB, Nielsen B, Boll EJ, Skjøt-Rasmussen L, Wellejus A, Jørgensen L o.a. Functional in vitro screening of probiotic strains for inoculation of piglets as a prophylactic measure towards Enterotoxigenic Escherichia coli infection. Journal of Microbiological Methods. 2021 jan;180. 106126. https://doi.org/10.1016/j.mimet.2020.106126

Author

Hansen, Lea Hübertz Birch ; Nielsen, B ; Boll, E J ; Skjøt-Rasmussen, L ; Wellejus, A ; Jørgensen, L ; Lauridsen, Charlotte ; Canibe, Nuria. / Functional in vitro screening of probiotic strains for inoculation of piglets as a prophylactic measure towards Enterotoxigenic Escherichia coli infection. I: Journal of Microbiological Methods. 2021 ; Bind 180.

Bibtex

@article{ff214b30e8504440baf476af49ffecf0,
title = "Functional in vitro screening of probiotic strains for inoculation of piglets as a prophylactic measure towards Enterotoxigenic Escherichia coli infection",
abstract = "Enterotoxigenic Escherichia coli (ETEC), being the major cause of post-weaning diarrhoea (PWD) in newlyweaned piglets, induces poor performance and economic losses in pig production. This functional in vitroscreening study investigated probiotic strains for use in suckling piglets as a prophylactic strategy towards PWD.Nine strains were evaluated based on their ability to: enhance intestinal epithelial barrier function, reduceadherence of ETEC F18 to intestinal cells, inhibit growth of ETEC F18, and grow on porcine milk oligosaccharides.Strains included in the screening were of the species Lactobacillus, Enterococcus, Bifidobacterium and Bacillus.Our in vitro screening demonstrated genus-, species and strain-specific differences in the mode of action ofthe tested probiotic strains. Some of the tested bifidobacteria were able to grow on the two porcine milk oligosaccharides,3′ -sialyllactose sodium salt (3{\textquoteright}SL) and Lacto-N-neotetraose (LNnT), whereas most lactic acidbacteria strains and both Bacillus subtilis strains failed to do so. All probiotic strains inhibited growth of ETEC F18on agar plates. All but the bifidobacteria reduced binding of ETEC F18 to Caco-2 cell monolayers, with theEnterococcus faecium strain having the most profound effect. All three lactic acid bacteria and Bifidobacteriumanimalis subsp. lactis counteracted the ETEC F18-induced permeability across Caco-2 cell monolayers with theE. faecium strain exhibiting the most pronounced protective effect. The findings from this in vitro screening studyindicate that, when selecting probiotic strains for suckling piglets as a prophylactic strategy towards PWD, itwould be advantageous to choose a multi-species product including strains with different modes of action inorder to increase the likelihood of achieving beneficial effects in vivo.",
keywords = "Probiotics, Screening, Enterotoxigenic Escherichia coli, Piglet, TEER, Porcine milk oligosaccharides",
author = "Hansen, {Lea H{\"u}bertz Birch} and B Nielsen and Boll, {E J} and L Skj{\o}t-Rasmussen and A Wellejus and L J{\o}rgensen and Charlotte Lauridsen and Nuria Canibe",
year = "2021",
month = jan,
doi = "10.1016/j.mimet.2020.106126",
language = "English",
volume = "180",
journal = "Journal of Microbiological Methods",
issn = "0167-7012",
publisher = "Elsevier BV",

}

RIS

TY - JOUR

T1 - Functional in vitro screening of probiotic strains for inoculation of piglets as a prophylactic measure towards Enterotoxigenic Escherichia coli infection

AU - Hansen, Lea Hübertz Birch

AU - Nielsen, B

AU - Boll, E J

AU - Skjøt-Rasmussen, L

AU - Wellejus, A

AU - Jørgensen, L

AU - Lauridsen, Charlotte

AU - Canibe, Nuria

PY - 2021/1

Y1 - 2021/1

N2 - Enterotoxigenic Escherichia coli (ETEC), being the major cause of post-weaning diarrhoea (PWD) in newlyweaned piglets, induces poor performance and economic losses in pig production. This functional in vitroscreening study investigated probiotic strains for use in suckling piglets as a prophylactic strategy towards PWD.Nine strains were evaluated based on their ability to: enhance intestinal epithelial barrier function, reduceadherence of ETEC F18 to intestinal cells, inhibit growth of ETEC F18, and grow on porcine milk oligosaccharides.Strains included in the screening were of the species Lactobacillus, Enterococcus, Bifidobacterium and Bacillus.Our in vitro screening demonstrated genus-, species and strain-specific differences in the mode of action ofthe tested probiotic strains. Some of the tested bifidobacteria were able to grow on the two porcine milk oligosaccharides,3′ -sialyllactose sodium salt (3’SL) and Lacto-N-neotetraose (LNnT), whereas most lactic acidbacteria strains and both Bacillus subtilis strains failed to do so. All probiotic strains inhibited growth of ETEC F18on agar plates. All but the bifidobacteria reduced binding of ETEC F18 to Caco-2 cell monolayers, with theEnterococcus faecium strain having the most profound effect. All three lactic acid bacteria and Bifidobacteriumanimalis subsp. lactis counteracted the ETEC F18-induced permeability across Caco-2 cell monolayers with theE. faecium strain exhibiting the most pronounced protective effect. The findings from this in vitro screening studyindicate that, when selecting probiotic strains for suckling piglets as a prophylactic strategy towards PWD, itwould be advantageous to choose a multi-species product including strains with different modes of action inorder to increase the likelihood of achieving beneficial effects in vivo.

AB - Enterotoxigenic Escherichia coli (ETEC), being the major cause of post-weaning diarrhoea (PWD) in newlyweaned piglets, induces poor performance and economic losses in pig production. This functional in vitroscreening study investigated probiotic strains for use in suckling piglets as a prophylactic strategy towards PWD.Nine strains were evaluated based on their ability to: enhance intestinal epithelial barrier function, reduceadherence of ETEC F18 to intestinal cells, inhibit growth of ETEC F18, and grow on porcine milk oligosaccharides.Strains included in the screening were of the species Lactobacillus, Enterococcus, Bifidobacterium and Bacillus.Our in vitro screening demonstrated genus-, species and strain-specific differences in the mode of action ofthe tested probiotic strains. Some of the tested bifidobacteria were able to grow on the two porcine milk oligosaccharides,3′ -sialyllactose sodium salt (3’SL) and Lacto-N-neotetraose (LNnT), whereas most lactic acidbacteria strains and both Bacillus subtilis strains failed to do so. All probiotic strains inhibited growth of ETEC F18on agar plates. All but the bifidobacteria reduced binding of ETEC F18 to Caco-2 cell monolayers, with theEnterococcus faecium strain having the most profound effect. All three lactic acid bacteria and Bifidobacteriumanimalis subsp. lactis counteracted the ETEC F18-induced permeability across Caco-2 cell monolayers with theE. faecium strain exhibiting the most pronounced protective effect. The findings from this in vitro screening studyindicate that, when selecting probiotic strains for suckling piglets as a prophylactic strategy towards PWD, itwould be advantageous to choose a multi-species product including strains with different modes of action inorder to increase the likelihood of achieving beneficial effects in vivo.

KW - Probiotics

KW - Screening

KW - Enterotoxigenic Escherichia coli

KW - Piglet

KW - TEER

KW - Porcine milk oligosaccharides

U2 - 10.1016/j.mimet.2020.106126

DO - 10.1016/j.mimet.2020.106126

M3 - Journal article

C2 - 33333101

VL - 180

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

M1 - 106126

ER -