Functional consequences of T-stem mutations in E. coli tRNA Thr UGU in vitro and in vivo

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Functional consequences of T-stem mutations in E. coli tRNA Thr UGU in vitro and in vivo. / Saks, Margaret E; Sanderson, Lee E; Choi, Daniel S; Crosby, Catherine M; Uhlenbeck, Olke C.

I: RNA, Bind 17, Nr. 6, 01.06.2011, s. 1038-1047.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Saks, ME, Sanderson, LE, Choi, DS, Crosby, CM & Uhlenbeck, OC 2011, 'Functional consequences of T-stem mutations in E. coli tRNA Thr UGU in vitro and in vivo', RNA, bind 17, nr. 6, s. 1038-1047. https://doi.org/10.1261/rna.2427311

APA

Saks, M. E., Sanderson, L. E., Choi, D. S., Crosby, C. M., & Uhlenbeck, O. C. (2011). Functional consequences of T-stem mutations in E. coli tRNA Thr UGU in vitro and in vivo. RNA, 17(6), 1038-1047. https://doi.org/10.1261/rna.2427311

CBE

Saks ME, Sanderson LE, Choi DS, Crosby CM, Uhlenbeck OC. 2011. Functional consequences of T-stem mutations in E. coli tRNA Thr UGU in vitro and in vivo. RNA. 17(6):1038-1047. https://doi.org/10.1261/rna.2427311

MLA

Vancouver

Saks ME, Sanderson LE, Choi DS, Crosby CM, Uhlenbeck OC. Functional consequences of T-stem mutations in E. coli tRNA Thr UGU in vitro and in vivo. RNA. 2011 jun 1;17(6):1038-1047. https://doi.org/10.1261/rna.2427311

Author

Saks, Margaret E ; Sanderson, Lee E ; Choi, Daniel S ; Crosby, Catherine M ; Uhlenbeck, Olke C. / Functional consequences of T-stem mutations in E. coli tRNA Thr UGU in vitro and in vivo. I: RNA. 2011 ; Bind 17, Nr. 6. s. 1038-1047.

Bibtex

@article{caafdb2c267f41b192f476717c9b725e,
title = "Functional consequences of T-stem mutations in E. coli tRNA Thr UGU in vitro and in vivo",
abstract = "The binding affinities between Escherichia coli EF-Tu and 34 single and double base-pair changes in the T stem of E. coli tRNAThrUGU were compared with similar data obtained previously for several aa-tRNAs binding to Thermus thermophilus EF-Tu. With a single exception, the two proteins bound to mutations in three T-stem base pairs in a quantitatively identical manner. However, tRNAThr differs from other tRNAs by also using its rare A52–C62 pair as a negative specificity determinant. Using a plasmid-based tRNA gene replacement strategy, we show that many of the tRNAThrUGU T-stem changes are either unable to support growth of E. coli or are less effective than the wild-type sequence. Since the inviable T-stem sequences are often present in other E. coli tRNAs, it appears that T-stem sequences in each tRNA body have evolved to optimize function in a different way. Although mutations of tRNAThr can substantially increase or decrease its affinity to EF-Tu, the observed affinities do not correlate with the growth phenotype of the mutations in any simple way. This may either reflect the different conditions used in the two assays or indicate that the T-stem mutants affect another step in the translation mechanism",
author = "Saks, {Margaret E} and Sanderson, {Lee E} and Choi, {Daniel S} and Crosby, {Catherine M} and Uhlenbeck, {Olke C}",
note = "MEDLINE{\circledR} is the source for the MeSH terms of this document.",
year = "2011",
month = "6",
day = "1",
doi = "10.1261/rna.2427311",
language = "English",
volume = "17",
pages = "1038--1047",
journal = "RNA",
issn = "1355-8382",
publisher = "Cold Spring Harbor Laboratory Press",
number = "6",

}

RIS

TY - JOUR

T1 - Functional consequences of T-stem mutations in E. coli tRNA Thr UGU in vitro and in vivo

AU - Saks, Margaret E

AU - Sanderson, Lee E

AU - Choi, Daniel S

AU - Crosby, Catherine M

AU - Uhlenbeck, Olke C

N1 - MEDLINE® is the source for the MeSH terms of this document.

PY - 2011/6/1

Y1 - 2011/6/1

N2 - The binding affinities between Escherichia coli EF-Tu and 34 single and double base-pair changes in the T stem of E. coli tRNAThrUGU were compared with similar data obtained previously for several aa-tRNAs binding to Thermus thermophilus EF-Tu. With a single exception, the two proteins bound to mutations in three T-stem base pairs in a quantitatively identical manner. However, tRNAThr differs from other tRNAs by also using its rare A52–C62 pair as a negative specificity determinant. Using a plasmid-based tRNA gene replacement strategy, we show that many of the tRNAThrUGU T-stem changes are either unable to support growth of E. coli or are less effective than the wild-type sequence. Since the inviable T-stem sequences are often present in other E. coli tRNAs, it appears that T-stem sequences in each tRNA body have evolved to optimize function in a different way. Although mutations of tRNAThr can substantially increase or decrease its affinity to EF-Tu, the observed affinities do not correlate with the growth phenotype of the mutations in any simple way. This may either reflect the different conditions used in the two assays or indicate that the T-stem mutants affect another step in the translation mechanism

AB - The binding affinities between Escherichia coli EF-Tu and 34 single and double base-pair changes in the T stem of E. coli tRNAThrUGU were compared with similar data obtained previously for several aa-tRNAs binding to Thermus thermophilus EF-Tu. With a single exception, the two proteins bound to mutations in three T-stem base pairs in a quantitatively identical manner. However, tRNAThr differs from other tRNAs by also using its rare A52–C62 pair as a negative specificity determinant. Using a plasmid-based tRNA gene replacement strategy, we show that many of the tRNAThrUGU T-stem changes are either unable to support growth of E. coli or are less effective than the wild-type sequence. Since the inviable T-stem sequences are often present in other E. coli tRNAs, it appears that T-stem sequences in each tRNA body have evolved to optimize function in a different way. Although mutations of tRNAThr can substantially increase or decrease its affinity to EF-Tu, the observed affinities do not correlate with the growth phenotype of the mutations in any simple way. This may either reflect the different conditions used in the two assays or indicate that the T-stem mutants affect another step in the translation mechanism

UR - http://www.scopus.com/inward/record.url?scp=79956191990&partnerID=8YFLogxK

U2 - 10.1261/rna.2427311

DO - 10.1261/rna.2427311

M3 - Journal article

C2 - 21527672

AN - SCOPUS:79956191990

VL - 17

SP - 1038

EP - 1047

JO - RNA

JF - RNA

SN - 1355-8382

IS - 6

ER -