Function of type-2 Arabidopsis hemoglobin in the auxin-mediated formation of embryogenic cells during morphogenesis

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Function of type-2 Arabidopsis hemoglobin in the auxin-mediated formation of embryogenic cells during morphogenesis. / Elhiti, Mohamed; Hebelstrup, Kim; Wang, Aiming; Li, Chenlong; Cui, Yuhai; Hill, Robert D; Stasolla, Claudio.

I: Plant Journal, Bind 74, Nr. 6, 06.2013, s. 946-958.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Elhiti, M, Hebelstrup, K, Wang, A, Li, C, Cui, Y, Hill, RD & Stasolla, C 2013, 'Function of type-2 Arabidopsis hemoglobin in the auxin-mediated formation of embryogenic cells during morphogenesis' Plant Journal, bind 74, nr. 6, s. 946-958. https://doi.org/10.1111/tpj.12181

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Elhiti, Mohamed ; Hebelstrup, Kim ; Wang, Aiming ; Li, Chenlong ; Cui, Yuhai ; Hill, Robert D ; Stasolla, Claudio. / Function of type-2 Arabidopsis hemoglobin in the auxin-mediated formation of embryogenic cells during morphogenesis. I: Plant Journal. 2013 ; Bind 74, Nr. 6. s. 946-958.

Bibtex

@article{f5d9933cc43646a9872038a8020cf857,
title = "Function of type-2 Arabidopsis hemoglobin in the auxin-mediated formation of embryogenic cells during morphogenesis",
abstract = "Suppression of the Arabidopsis GLB2, a type-2 nonsymbiotic hemoglobin, enhances somatic embryogenesis by increasing auxin production. In the glb2 knock-out line (GLB2 -/-) polarization of PIN1 proteins and auxin maxima occurred at the base of the cotyledons of the zygotic explants, which are the sites of embryogenic tissue formation. These changes were also accompanied by a transcriptional up-regulation of WUSCHEL (WUS) and SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK1), markers of embryogenic competence. The increased auxin levels in the GLB2 -/- line were ascribed to the induction of several key enzymes of the tryptophan and IAA biosynthetic pathways, including ANTHRANILATE SYNTHASE (α subunit) (ASA1), CYTOCHROME P79B2 (CYP79B2) and AMIDASE1 (AMI1). The effects of GLB2 suppression on somatic embryogenesis and IAA synthesis are mediated by increasing levels of nitric oxide (NO) within the embryogenic cells, which repress the expression of the transcription factor MYC2, a well characterized repressor of the auxin biosynthetic pathway. A model is proposed in which the suppression of GLB2 reduces the degree of NO scavenging by oxyhemoglobin, thereby increasing the cellular NO concentration. The increased levels of NO repress the expression of MYC2, relieving the inhibition of IAA synthesis and increasing cellular IAA, which is the inductive signal promoting embryogenic competence. Besides providing a model for the induction phase of embryogenesis in vitro, these studies propose previously undescribed functions for plant hemoglobins.",
keywords = "auxin, hemoglobin, nitric oxide, somatic embryogenesis",
author = "Mohamed Elhiti and Kim Hebelstrup and Aiming Wang and Chenlong Li and Yuhai Cui and Hill, {Robert D} and Claudio Stasolla",
year = "2013",
month = "6",
doi = "10.1111/tpj.12181",
language = "English",
volume = "74",
pages = "946--958",
journal = "The Plant Journal",
issn = "0960-7412",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "6",

}

RIS

TY - JOUR

T1 - Function of type-2 Arabidopsis hemoglobin in the auxin-mediated formation of embryogenic cells during morphogenesis

AU - Elhiti, Mohamed

AU - Hebelstrup, Kim

AU - Wang, Aiming

AU - Li, Chenlong

AU - Cui, Yuhai

AU - Hill, Robert D

AU - Stasolla, Claudio

PY - 2013/6

Y1 - 2013/6

N2 - Suppression of the Arabidopsis GLB2, a type-2 nonsymbiotic hemoglobin, enhances somatic embryogenesis by increasing auxin production. In the glb2 knock-out line (GLB2 -/-) polarization of PIN1 proteins and auxin maxima occurred at the base of the cotyledons of the zygotic explants, which are the sites of embryogenic tissue formation. These changes were also accompanied by a transcriptional up-regulation of WUSCHEL (WUS) and SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK1), markers of embryogenic competence. The increased auxin levels in the GLB2 -/- line were ascribed to the induction of several key enzymes of the tryptophan and IAA biosynthetic pathways, including ANTHRANILATE SYNTHASE (α subunit) (ASA1), CYTOCHROME P79B2 (CYP79B2) and AMIDASE1 (AMI1). The effects of GLB2 suppression on somatic embryogenesis and IAA synthesis are mediated by increasing levels of nitric oxide (NO) within the embryogenic cells, which repress the expression of the transcription factor MYC2, a well characterized repressor of the auxin biosynthetic pathway. A model is proposed in which the suppression of GLB2 reduces the degree of NO scavenging by oxyhemoglobin, thereby increasing the cellular NO concentration. The increased levels of NO repress the expression of MYC2, relieving the inhibition of IAA synthesis and increasing cellular IAA, which is the inductive signal promoting embryogenic competence. Besides providing a model for the induction phase of embryogenesis in vitro, these studies propose previously undescribed functions for plant hemoglobins.

AB - Suppression of the Arabidopsis GLB2, a type-2 nonsymbiotic hemoglobin, enhances somatic embryogenesis by increasing auxin production. In the glb2 knock-out line (GLB2 -/-) polarization of PIN1 proteins and auxin maxima occurred at the base of the cotyledons of the zygotic explants, which are the sites of embryogenic tissue formation. These changes were also accompanied by a transcriptional up-regulation of WUSCHEL (WUS) and SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK1), markers of embryogenic competence. The increased auxin levels in the GLB2 -/- line were ascribed to the induction of several key enzymes of the tryptophan and IAA biosynthetic pathways, including ANTHRANILATE SYNTHASE (α subunit) (ASA1), CYTOCHROME P79B2 (CYP79B2) and AMIDASE1 (AMI1). The effects of GLB2 suppression on somatic embryogenesis and IAA synthesis are mediated by increasing levels of nitric oxide (NO) within the embryogenic cells, which repress the expression of the transcription factor MYC2, a well characterized repressor of the auxin biosynthetic pathway. A model is proposed in which the suppression of GLB2 reduces the degree of NO scavenging by oxyhemoglobin, thereby increasing the cellular NO concentration. The increased levels of NO repress the expression of MYC2, relieving the inhibition of IAA synthesis and increasing cellular IAA, which is the inductive signal promoting embryogenic competence. Besides providing a model for the induction phase of embryogenesis in vitro, these studies propose previously undescribed functions for plant hemoglobins.

KW - auxin

KW - hemoglobin

KW - nitric oxide

KW - somatic embryogenesis

U2 - 10.1111/tpj.12181

DO - 10.1111/tpj.12181

M3 - Journal article

VL - 74

SP - 946

EP - 958

JO - The Plant Journal

JF - The Plant Journal

SN - 0960-7412

IS - 6

ER -