Full membrane spanning self-assembled monolayers as model systems for UHV-based studies of cell-penetrating peptides

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  • Johannes Franz, Max Planck Inst Polymer Res, Max Planck Society
  • ,
  • Daniel J. Graham, Univ Washington, University of Washington, University of Washington Seattle, NESAC BIO
  • ,
  • Lars Schmüser, Max Planck Inst Polymer Res, Max Planck Society
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  • Joe E. Baio, Oregon State Univ, Oregon State University, Oregon University System, Sch Chem Biol & Environm Engn
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  • Marco Lelle, Max Planck Inst Polymer Res, Max Planck Society
  • ,
  • Kalina Peneva, Max Planck Inst Polymer Res, Max Planck Society
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  • Klaus Muellen, Max Planck Inst Polymer Res, Max Planck Society
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  • David G. Castner, Univ Washington, University of Washington, University of Washington Seattle, NESAC BIO
  • ,
  • Mischa Bonn, Max Planck Inst Polymer Res, Max Planck Society
  • ,
  • Tobias Weidner

Biophysical studies of the interaction of peptides with model membranes provide a simple yet effective approach to understand the transport of peptides and peptide based drug carriers across the cell membrane. Herein, the authors discuss the use of self-assembled monolayers fabricated from the full membrane-spanning thiol (FMST) 3-((14-((40-((5-methyl-1-phenyl-35-(phytanyl) oxy6,9,12,15,18,21,24,27,30,33,37- undecaoxa-2,3-dithiahenpentacontan-51-yl) oxy)-[1,10-biphenyl]-4yl) oxy) tetradecyl) oxy)-2-(phytanyl) oxy glycerol for ultrahigh vacuum (UHV) based experiments. UHV-based methods such as electron spectroscopy and mass spectrometry can provide important information about how peptides bind and interact with membranes, especially with the hydrophobic core of a lipid bilayer. Near-edge x-ray absorption fine structure spectra and x-ray photoelectron spectroscopy (XPS) data showed that FMST forms UHV-stable and ordered films on gold. XPS and time of flight secondary ion mass spectrometry depth profiles indicated that a proline-rich amphipathic cell-penetrating peptide, known as sweet arrow peptide is located at the outer perimeter of the model membrane. (C) 2015 American Vacuum Society.

OriginalsprogEngelsk
Artikelnummer019009
TidsskriftBiointerphases
Vol/bind10
Nummer1
Antal sider6
ISSN1934-8630
DOI
StatusUdgivet - mar. 2015
Eksternt udgivetJa

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