Folic Acid Conjugated Chitosan for Targeted Delivery of siRNA to Activated Macrophages in vitro and in vivo

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Folic Acid Conjugated Chitosan for Targeted Delivery of siRNA to Activated Macrophages in vitro and in vivo. / Yang, Chuanxu; Gao, Shan; Kjems, Jørgen.

I: Journal of Materials Chemistry B, Bind 48, 10.2014, s. 8608-8615.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

APA

CBE

MLA

Vancouver

Author

Yang, Chuanxu ; Gao, Shan ; Kjems, Jørgen. / Folic Acid Conjugated Chitosan for Targeted Delivery of siRNA to Activated Macrophages in vitro and in vivo. I: Journal of Materials Chemistry B. 2014 ; Bind 48. s. 8608-8615.

Bibtex

@article{f6767d12dbb44ce7be6616c30148708b,
title = "Folic Acid Conjugated Chitosan for Targeted Delivery of siRNA to Activated Macrophages in vitro and in vivo",
abstract = "Activated macrophages play an important role in the initiation and development of inflammatory diseases. The aim of this study is to develop a delivery system that targets siRNA to activated macrophages. Exploiting the presence of folate receptors on the surface of activated macrophages, folic acid was conjugated to chitosan (FA–CS) and used to formulate siRNA into nanoparticles capable of cell specific delivery. The physiochemical properties of the nanoparticles, including size, zeta-potential and encapsulation efficiency, were characterized and the intracellular uptake and gene silencing efficiency were studied in vitro. The results showed that folic acid conjugation enhanced cellular uptake and silencing effect in activated macrophages. An in vivo biodistribution analysis, performed in a subcutaneous inflammation model, confirmed targeting of FA–CS/siRNA to inflamed tissue. The results indicate that FA–CS can be a potential siRNA carrier for anti-inflammatory therapy",
author = "Chuanxu Yang and Shan Gao and J{\o}rgen Kjems",
year = "2014",
month = oct,
doi = "10.1039/C4TB01374C",
language = "English",
volume = "48",
pages = "8608--8615",
journal = "Journal of Materials Chemistry B",
issn = "2050-750X",
publisher = "ROYAL SOC CHEMISTRY",

}

RIS

TY - JOUR

T1 - Folic Acid Conjugated Chitosan for Targeted Delivery of siRNA to Activated Macrophages in vitro and in vivo

AU - Yang, Chuanxu

AU - Gao, Shan

AU - Kjems, Jørgen

PY - 2014/10

Y1 - 2014/10

N2 - Activated macrophages play an important role in the initiation and development of inflammatory diseases. The aim of this study is to develop a delivery system that targets siRNA to activated macrophages. Exploiting the presence of folate receptors on the surface of activated macrophages, folic acid was conjugated to chitosan (FA–CS) and used to formulate siRNA into nanoparticles capable of cell specific delivery. The physiochemical properties of the nanoparticles, including size, zeta-potential and encapsulation efficiency, were characterized and the intracellular uptake and gene silencing efficiency were studied in vitro. The results showed that folic acid conjugation enhanced cellular uptake and silencing effect in activated macrophages. An in vivo biodistribution analysis, performed in a subcutaneous inflammation model, confirmed targeting of FA–CS/siRNA to inflamed tissue. The results indicate that FA–CS can be a potential siRNA carrier for anti-inflammatory therapy

AB - Activated macrophages play an important role in the initiation and development of inflammatory diseases. The aim of this study is to develop a delivery system that targets siRNA to activated macrophages. Exploiting the presence of folate receptors on the surface of activated macrophages, folic acid was conjugated to chitosan (FA–CS) and used to formulate siRNA into nanoparticles capable of cell specific delivery. The physiochemical properties of the nanoparticles, including size, zeta-potential and encapsulation efficiency, were characterized and the intracellular uptake and gene silencing efficiency were studied in vitro. The results showed that folic acid conjugation enhanced cellular uptake and silencing effect in activated macrophages. An in vivo biodistribution analysis, performed in a subcutaneous inflammation model, confirmed targeting of FA–CS/siRNA to inflamed tissue. The results indicate that FA–CS can be a potential siRNA carrier for anti-inflammatory therapy

U2 - 10.1039/C4TB01374C

DO - 10.1039/C4TB01374C

M3 - Journal article

VL - 48

SP - 8608

EP - 8615

JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

SN - 2050-750X

ER -