Focal Adhesion Kinase Knockdown in Carcinoma-Associated Fibroblasts Inhibits Oral Squamous Cell Carcinoma Metastasis via Downregulating MCP-1/CCL2 Expression

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  • Anjie Min, Cent S Univ, Central South University, Xiangya Hosp, Dept Oral & Maxillofacial Surg
  • ,
  • Chao Zhu, Cent S Univ, Sch Stomatol
  • ,
  • Jingyi Wang, Univ Hong Kong, University of Hong Kong, Fac Dent
  • ,
  • Shuping Peng, Cent S Univ, Central South University, Canc Res Inst
  • ,
  • Cijun Shuai, Cent S Univ, Central South University, State Key Lab High Performance Complex Mfg
  • ,
  • Shan Gao
  • ,
  • Zhangui Tang, Cent S Univ, Central South University, Xiangya Stomatol Hosp, Dept Oral & Maxillofacial Surg
  • ,
  • Tong Su, Cent S Univ, Central South University, Xiangya Hosp, Dept Oral & Maxillofacial Surg

Carcinoma-associated fibroblasts (CAFs) have been demonstrated to play an important role in the occurrence and development of oral squamous cell carcinoma (OSCC). The aim of this study is to investigate the influence of CAFs on OSCC cells and to explore the role of focal adhesion kinase (FAK) in this process. The results showed that oral CAFs expressed a higher level of FAK than normal human gingival fibroblasts (HGFs), and the conditioned medium (CM) of CAFs could induce the invasion and migration of SCC-25, one oral squamous carcinoma cell line. However, knockdown of FAK by small interfering RNA (siRNA) resulted in inhibition of CAF-CM induced cell invasion and migration in SCC-25, probably by reducing the production of monocyte chemoattractant protein-1 (MCP-1/CCL2), one of downstream target chemokines. Therefore, our findings indicated that targeting FAK in CAFs might be a promising strategy for the treatment of OSCC in the future. (C) 2014 Wiley Periodicals, Inc.

OriginalsprogEngelsk
TidsskriftJournal of Biochemical and Molecular Toxicology (Print Edition)
Vol/bind29
Nummer2
Sider (fra-til)70-76
Antal sider7
ISSN1095-6670
DOI
StatusUdgivet - feb. 2015

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