Fluorescence detection of natural RNA using rationally designed "clickable" oligonucleotide probes

Anders Okholm; Jørgen Kjems; Kira Astakhova

doi:10.1039/c4ra07165d

Fluorescence detection of natural RNA using rationally designed "clickable" oligonucleotide probes

Anders Okholm, Jørgen Kjems, Kira Astakhova^*

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

10 Citationer (Scopus)

Abstract

Herein a reliable approach to the design of effective fluorescent probes for RNA detection is described. The fluorescence signalling of hybridization by internally positioned polyaromatic hydrocarbons and rhodamine dyes was achieved with a low fluorescence background signal, high fluorescence quantum yields at ambient and elevated temperature, high selectivity and signal specificity of the probes when binding to miR-7 and circRNA targets. This journal is

Originalsprog	Engelsk
Tidsskrift	RSC Advances
Vol/bind	4
Nummer	86
Sider (fra-til)	45653-45656
Antal sider	4
ISSN	2046-2069
DOI	https://doi.org/10.1039/c4ra07165d
Status	Udgivet - 2014

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AU - Okholm, Anders

AU - Kjems, Jørgen

AU - Astakhova, Kira

PY - 2014

Y1 - 2014

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AB - Herein a reliable approach to the design of effective fluorescent probes for RNA detection is described. The fluorescence signalling of hybridization by internally positioned polyaromatic hydrocarbons and rhodamine dyes was achieved with a low fluorescence background signal, high fluorescence quantum yields at ambient and elevated temperature, high selectivity and signal specificity of the probes when binding to miR-7 and circRNA targets. This journal is

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U2 - 10.1039/c4ra07165d

DO - 10.1039/c4ra07165d

M3 - Journal article

AN - SCOPUS:84907831103

SN - 2046-2069

VL - 4

SP - 45653

EP - 45656

JO - RSC Advances

JF - RSC Advances

IS - 86

ER -

Fluorescence detection of natural RNA using rationally designed "clickable" oligonucleotide probes

Abstract

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