Fkbp8: novel isoforms, genomic organization, and characterization of a forebrain promoter in transgenic mice

Jakob V. Nielsen; Cathy Mitchelmore; Karen-Marie Pedersen; Karen M Kjaerulff; Bente Finsen; Niels Jensen

Fkbp8: novel isoforms, genomic organization, and characterization of a forebrain promoter in transgenic mice

Jakob V. Nielsen, Cathy Mitchelmore, Karen-Marie Pedersen, Karen M Kjaerulff, Bente Finsen, Niels Jensen

Institut for Biomedicin - Forskning og uddannelse, Vest

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

27 Citationer (Scopus)

Abstract

The immunophilin homolog FKBP8 has been implicated in the regulation of apoptosis. Here we show that the 38-kDa form of FKBP8 (FKBP38) derives from a truncated ORF. The extended FKBP8 ORFs are 46 and 44 kDa in mouse and 45 kDa in human. Although the genomic organization of mouse and human FKBP8 is evolutionarily conserved, additional first exons are encoded by the murine locus. A 4.4-kb murine Fkbp8 gene fragment, containing a GC-rich potential promoter, directed expression of a LacZ reporter gene to forebrain neurons in transgenic mice. Expression of the transgene was observed in CA1 pyramidal neurons of the hippocampus in transgenic mice from three lines. One transgenic founder mouse exhibited widespread forebrain expression of the LacZ transgene that resembles the pattern for the endogenous Fkbp8 gene. Thus promoter/enhancer elements for forebrain expression are located around the first exons of the mouse Fkbp8 gene.

Originalsprog	Engelsk
Tidsskrift	Genomics
Vol/bind	83
Nummer	1
Sider (fra-til)	181-92
Antal sider	12
ISSN	0888-7543
Status	Udgivet - 2004

Citationsformater

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title = "Fkbp8: novel isoforms, genomic organization, and characterization of a forebrain promoter in transgenic mice",

abstract = "The immunophilin homolog FKBP8 has been implicated in the regulation of apoptosis. Here we show that the 38-kDa form of FKBP8 (FKBP38) derives from a truncated ORF. The extended FKBP8 ORFs are 46 and 44 kDa in mouse and 45 kDa in human. Although the genomic organization of mouse and human FKBP8 is evolutionarily conserved, additional first exons are encoded by the murine locus. A 4.4-kb murine Fkbp8 gene fragment, containing a GC-rich potential promoter, directed expression of a LacZ reporter gene to forebrain neurons in transgenic mice. Expression of the transgene was observed in CA1 pyramidal neurons of the hippocampus in transgenic mice from three lines. One transgenic founder mouse exhibited widespread forebrain expression of the LacZ transgene that resembles the pattern for the endogenous Fkbp8 gene. Thus promoter/enhancer elements for forebrain expression are located around the first exons of the mouse Fkbp8 gene.",

keywords = "Alternative Splicing, Amino Acid Sequence, Animals, Base Sequence, Cerebellum, Corpus Striatum, DNA, Exons, Female, Gene Expression, Genes, Hippocampus, Humans, In Situ Hybridization, Introns, Lac Operon, Male, Mice, Mice, Inbred Strains, Mice, Transgenic, Molecular Sequence Data, Neocortex, Promoter Regions, Genetic, Prosencephalon, Protein Isoforms, Pyramidal Cells, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Tacrolimus Binding Proteins, Transcription Initiation Site",

author = "Nielsen, {Jakob V.} and Cathy Mitchelmore and Karen-Marie Pedersen and Kjaerulff, {Karen M} and Bente Finsen and Niels Jensen",

year = "2004",

language = "English",

volume = "83",

pages = "181--92",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

number = "1",

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TY - JOUR

T1 - Fkbp8: novel isoforms, genomic organization, and characterization of a forebrain promoter in transgenic mice

AU - Nielsen, Jakob V.

AU - Mitchelmore, Cathy

AU - Pedersen, Karen-Marie

AU - Kjaerulff, Karen M

AU - Finsen, Bente

AU - Jensen, Niels

PY - 2004

Y1 - 2004

N2 - The immunophilin homolog FKBP8 has been implicated in the regulation of apoptosis. Here we show that the 38-kDa form of FKBP8 (FKBP38) derives from a truncated ORF. The extended FKBP8 ORFs are 46 and 44 kDa in mouse and 45 kDa in human. Although the genomic organization of mouse and human FKBP8 is evolutionarily conserved, additional first exons are encoded by the murine locus. A 4.4-kb murine Fkbp8 gene fragment, containing a GC-rich potential promoter, directed expression of a LacZ reporter gene to forebrain neurons in transgenic mice. Expression of the transgene was observed in CA1 pyramidal neurons of the hippocampus in transgenic mice from three lines. One transgenic founder mouse exhibited widespread forebrain expression of the LacZ transgene that resembles the pattern for the endogenous Fkbp8 gene. Thus promoter/enhancer elements for forebrain expression are located around the first exons of the mouse Fkbp8 gene.

AB - The immunophilin homolog FKBP8 has been implicated in the regulation of apoptosis. Here we show that the 38-kDa form of FKBP8 (FKBP38) derives from a truncated ORF. The extended FKBP8 ORFs are 46 and 44 kDa in mouse and 45 kDa in human. Although the genomic organization of mouse and human FKBP8 is evolutionarily conserved, additional first exons are encoded by the murine locus. A 4.4-kb murine Fkbp8 gene fragment, containing a GC-rich potential promoter, directed expression of a LacZ reporter gene to forebrain neurons in transgenic mice. Expression of the transgene was observed in CA1 pyramidal neurons of the hippocampus in transgenic mice from three lines. One transgenic founder mouse exhibited widespread forebrain expression of the LacZ transgene that resembles the pattern for the endogenous Fkbp8 gene. Thus promoter/enhancer elements for forebrain expression are located around the first exons of the mouse Fkbp8 gene.

KW - Alternative Splicing

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - Cerebellum

KW - Corpus Striatum

KW - DNA

KW - Exons

KW - Female

KW - Gene Expression

KW - Genes

KW - Hippocampus

KW - Humans

KW - In Situ Hybridization

KW - Introns

KW - Lac Operon

KW - Male

KW - Mice

KW - Mice, Inbred Strains

KW - Mice, Transgenic

KW - Molecular Sequence Data

KW - Neocortex

KW - Promoter Regions, Genetic

KW - Prosencephalon

KW - Protein Isoforms

KW - Pyramidal Cells

KW - Sequence Alignment

KW - Sequence Analysis, DNA

KW - Sequence Homology, Amino Acid

KW - Sequence Homology, Nucleic Acid

KW - Tacrolimus Binding Proteins

KW - Transcription Initiation Site

M3 - Journal article

C2 - 14667822

SN - 0888-7543

VL - 83

SP - 181

EP - 192

JO - Genomics

JF - Genomics

IS - 1

ER -

Fkbp8: novel isoforms, genomic organization, and characterization of a forebrain promoter in transgenic mice

Abstract

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