FERONIA restricts Pseudomonas in the rhizosphere microbiome via regulation of reactive oxygen species

Yi Song; Andrew J. Wilson; Xue Cheng Zhang; David Thoms; Reza Sohrabi; Siyu Song; Quentin Geissmann; Yang Liu; Lauren Walgren; Sheng Yang He; Cara H. Haney

doi:10.1038/s41477-021-00914-0

FERONIA restricts Pseudomonas in the rhizosphere microbiome via regulation of reactive oxygen species

Yi Song, Andrew J. Wilson, Xue Cheng Zhang, David Thoms, Reza Sohrabi, Siyu Song, Quentin Geissmann, Yang Liu, Lauren Walgren, Sheng Yang He, Cara H. Haney^*

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

151 Citationer (Scopus)

Abstract

Maintaining microbiome structure is critical for the health of both plants and animals. By re-screening a collection of Arabidopsis mutants affecting root immunity and hormone crosstalk, we identified a FERONIA (FER) receptor kinase mutant (fer-8) with a rhizosphere microbiome enriched in Pseudomonas fluorescens without phylum-level dysbiosis. Using microbiome transplant experiments, we found that the fer-8 microbiome was beneficial. The effect of FER on rhizosphere pseudomonads was largely independent of its immune scaffold function, role in development and jasmonic acid autoimmunity. We found that the fer-8 mutant has reduced basal levels of reactive oxygen species (ROS) in roots and that mutants deficient in NADPH oxidase showed elevated rhizosphere pseudomonads. The addition of RALF23 peptides, a FER ligand, was sufficient to enrich P. fluorescens. This work shows that FER-mediated ROS production regulates levels of beneficial pseudomonads in the rhizosphere microbiome.

Originalsprog	Engelsk
Tidsskrift	Nature Plants
Vol/bind	7
Nummer	5
Sider (fra-til)	644-654
Antal sider	11
ISSN	2055-0278
DOI	https://doi.org/10.1038/s41477-021-00914-0
Status	Udgivet - maj 2021
Udgivet eksternt	Ja

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abstract = "Maintaining microbiome structure is critical for the health of both plants and animals. By re-screening a collection of Arabidopsis mutants affecting root immunity and hormone crosstalk, we identified a FERONIA (FER) receptor kinase mutant (fer-8) with a rhizosphere microbiome enriched in Pseudomonas fluorescens without phylum-level dysbiosis. Using microbiome transplant experiments, we found that the fer-8 microbiome was beneficial. The effect of FER on rhizosphere pseudomonads was largely independent of its immune scaffold function, role in development and jasmonic acid autoimmunity. We found that the fer-8 mutant has reduced basal levels of reactive oxygen species (ROS) in roots and that mutants deficient in NADPH oxidase showed elevated rhizosphere pseudomonads. The addition of RALF23 peptides, a FER ligand, was sufficient to enrich P. fluorescens. This work shows that FER-mediated ROS production regulates levels of beneficial pseudomonads in the rhizosphere microbiome.",

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AU - Wilson, Andrew J.

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AU - Thoms, David

AU - Sohrabi, Reza

AU - Song, Siyu

AU - Geissmann, Quentin

AU - Liu, Yang

AU - Walgren, Lauren

AU - He, Sheng Yang

AU - Haney, Cara H.

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N2 - Maintaining microbiome structure is critical for the health of both plants and animals. By re-screening a collection of Arabidopsis mutants affecting root immunity and hormone crosstalk, we identified a FERONIA (FER) receptor kinase mutant (fer-8) with a rhizosphere microbiome enriched in Pseudomonas fluorescens without phylum-level dysbiosis. Using microbiome transplant experiments, we found that the fer-8 microbiome was beneficial. The effect of FER on rhizosphere pseudomonads was largely independent of its immune scaffold function, role in development and jasmonic acid autoimmunity. We found that the fer-8 mutant has reduced basal levels of reactive oxygen species (ROS) in roots and that mutants deficient in NADPH oxidase showed elevated rhizosphere pseudomonads. The addition of RALF23 peptides, a FER ligand, was sufficient to enrich P. fluorescens. This work shows that FER-mediated ROS production regulates levels of beneficial pseudomonads in the rhizosphere microbiome.

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KW - Phosphotransferases/metabolism

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KW - Reactive Oxygen Species/metabolism

KW - Rhizosphere

KW - Soil Microbiology

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FERONIA restricts Pseudomonas in the rhizosphere microbiome via regulation of reactive oxygen species

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