Fast and Quantitative NMR Metabolite Analysis Afforded by a Paramagnetic Co-Solute

Frans A.A. Mulder*, Leonardo Tenori, Claudio Luchinat

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

30 Citationer (Scopus)

Abstract

NMR spectroscopy is an indispensable technique for the determination of the chemical identity and structure of small molecules. The method is especially recognized for its robustness and intrinsically quantitative nature, and has manifested itself as a key analytical platform for diverse fields of application, ranging from chemical synthesis to metabolomics. Unfortunately, the slow recovery of nuclear spin polarization by spin-lattice (T1) relaxation causes most experimental time to be lost on idle waiting. Furthermore, truly quantitative NMR (qNMR) spectroscopy requires waiting times of 5-times the longest T1 in the sample, making qNMR spectroscopy slow and inefficient. We demonstrate here that co-solute paramagnetic relaxation can mitigate these two problems simultaneously. The addition of a small amount of paramagnetic gadolinium chelate, available in the form of commercial contrast-agent solutions, enables cheap, quantitative, and efficient high-throughput mixture analysis.

OriginalsprogEngelsk
TidsskriftAngewandte Chemie - International Edition
Vol/bind58
Nummer43
Sider (fra-til)15283-15286
Antal sider4
ISSN1433-7851
DOI
StatusUdgivet - 2019

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