Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing

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DOI

  • Hassan Bardania, Yasuj University of Medical Sciences
  • ,
  • Reza Mahmoudi, Yasuj University of Medical Sciences
  • ,
  • Hamed Bagheri, Tarbiat Modares University
  • ,
  • Zeinab Salehpour, Yasuj University of Medical Sciences
  • ,
  • Mohamad Hassan Fouani, Tarbiat Modares University
  • ,
  • Bita Darabian, Tarbiat Modares University
  • ,
  • Seyed Sajjad Khoramrooz, Yasuj University of Medical Sciences
  • ,
  • Ali Mousavizadeh, Yasuj University of Medical Sciences
  • ,
  • Majid Kowsari, Jahrom University of Medical Science
  • ,
  • Seyyed Ebrahim Moosavifard, Jahrom University of Medical Science
  • ,
  • Gunna Christiansen
  • Danesh Javeshghani, Jahrom University of Medical Science
  • ,
  • Mohsen Alipour, Jahrom University of Medical Science
  • ,
  • Mohammad Akrami, Tehran University of Medical Sciences

To eliminate the microbial infection from an injury site, various modalities have been developed such as dressings and human skin substitutes. However, the high amount of reactive oxygen species, microbial infection, and damaging extracellular matrix remain as the main challenges for the wound healing process. In this study, for the first time, green synthesized silver nanoparticles (AgNPs) using Teucrium polium extract were embedded in poly lactic acid/poly ethylene glycol (PLA/PEG) film to provide absorbable wound dressing, with antioxidant and antibacterial features. The physicochemical analysis demonstrated, production of AgNPs with size approximately 32.2 nm and confirmed the presence of phytoconstituents on their surface. The antibacterial assessments exhibited a concentration-dependent sensitivity of Staphylococcus aureus and Pseudomonas aeruginosa toward biosynthesized AgNPs, which showed a suitable safety profile in human macrophage cells. Furthermore, oxidant scavenging assays demonstrated exploitation of plant extract as a reducing agent, endows antioxidant activity to biogenic AgNPs. The formation of PLA/PEG nanofilm and entrapment of AgNPs into their matrix were clearly confirmed by scanning electron microscopy. More importantly, antibacterial examination demonstrated that the introduction of biogenic AgNPs into PLA/PEG nanofibers led to complete growth inhibition of P. aeruginosa and S. aureus. In summary, the simultaneous antioxidant activity and antimicrobial activity of the novel biogenic AgNPs/PLA/PEG nanofilm showed its potential for application as wound dressing.

OriginalsprogEngelsk
Artikelnummer6129
TidsskriftScientific Reports
Vol/bind10
ISSN2045-2322
DOI
StatusUdgivet - apr. 2020

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