Extracting physiological information in experimental biology via Eulerian video magnification

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

  • Henrik Lauridsen
  • Selina Gonzales, California State University, Cornell University, USA
  • Daniela Hedwig, Cornell Univ, Cornell Lab Ornithol, USA
  • Kathryn L. Perrin, Center for Zoo and Wild Animal Health, Copenhagen Zoo, Frederiksberg, Copenhagen University, Danmark
  • Catherine Williams
  • Peter H. Wrege, Cornell Univ, Cornell Lab Ornithol, USA
  • Mads F. Bertelsen, Center for Zoo and Wild Animal Health, Copenhagen Zoo, Frederiksberg, Copenhagen University, Danmark
  • Michael Pedersen
  • Jonathan T. Butcher, The Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA, USA
Background:Videographic material of animals can contain inapparent signals, such as color changes or motionthat hold information about physiological functions, such as heart and respiration rate, pulse wave velocity, andvocalization. Eulerian video magnification allows the enhancement of such signals to enable their detection. Thepurpose of this study is to demonstrate how signals relevant to experimental physiology can be extracted fromnon-contact videographic material of animals.

Results:We applied Eulerian video magnification to detect physiological signals in a range of experimental modelsand in captive and free ranging wildlife. Neotenic Mexican axolotls were studied to demonstrate the extraction ofheart rate signal of non-embryonic animals from dedicated videographic material. Heart rate could be acquiredboth in single and multiple animal setups of leucistic and normally colored animals under different physiologicalconditions (resting, exercised, or anesthetized) using a wide range of video qualities. Pulse wave velocity could alsobe measured in the low blood pressure system of the axolotl as well as in the high-pressure system of the humanbeing. Heart rate extraction was also possible from videos of conscious, unconstrained zebrafish and from non-dedicated videographic material of sand lizard and giraffe. This technique also allowed for heart rate detection inembryonic chickens in ovo through the eggshell and in embryonic mice in utero and could be used as a gatingsignal to acquire two-phase volumetric micro-CT data of the beating embryonic chicken heart. Additionally, Eulerianvideo magnification was used to demonstrate how vocalization-induced vibrations can be detected in infrasound-producing Asian elephants.

Conclusions:Eulerian video magnification provides a technique to extract inapparent temporal signals fromvideographic material of animals. This can be applied in experimental and comparative physiology where contact-based recordings (e.g., heart rate) cannot be acquired.

Keywords:Videographic material, Signal processing, Heart rate, Respiration rate, Pulse wave velocity, Embryonicdevelopment, Infrasound
OriginalsprogEngelsk
Artikelnummer103
TidsskriftB M C Biology
Vol/bind17
ISSN1741-7007
DOI
StatusUdgivet - 12 dec. 2019

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