Extracellular matrix remodelling during cell adhesion monitored by the quartz crystal microbalance

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Extracellular matrix remodelling during cell adhesion monitored by the quartz crystal microbalance. / Lord, Megan S; Modin, Charlotte; Foss, Morten; Duch, Mogens; Simmons, Anne; Pedersen, Finn S; Besenbacher, Flemming; Milthorpe, Bruce K.

I: Biomaterials, Bind 29, Nr. 17, 2008, s. 2581-7.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Lord, Megan S ; Modin, Charlotte ; Foss, Morten ; Duch, Mogens ; Simmons, Anne ; Pedersen, Finn S ; Besenbacher, Flemming ; Milthorpe, Bruce K. / Extracellular matrix remodelling during cell adhesion monitored by the quartz crystal microbalance. I: Biomaterials. 2008 ; Bind 29, Nr. 17. s. 2581-7.

Bibtex

@article{8bae4e200c1f11dfb95d000ea68e967b,
title = "Extracellular matrix remodelling during cell adhesion monitored by the quartz crystal microbalance",
abstract = "A cell's ability to remodel adsorbed protein layers on surfaces is influenced by the nature of the protein layer itself. Remodelling is often required to accomplish cellular adhesion and extracellular matrix formation which forms the basis for cell spreading, increased adhesion and expression of different phenotypes. The adhesion of NIH3T3 (EGFP) fibroblasts to serum protein (albumin or fibronectin) precoated tantalum (Ta) and oxidised polystyrene (PS(ox)) surfaces was examined using the quartz crystal microbalance with dissipation (QCM-D) monitoring and fluorescence microscopy. The cells were either untreated or treated with cycloheximide to examine the contribution of endogenous protein production during cell adhesion to the QCM-D response over a period of 2h. Following adsorption of albumin onto Ta and PS(ox) there was no difference detected between the response to seeding untreated and cycloheximide treated cells. The QCM-D was able to detect differences in the untreated cellular responses to fibronectin versus serum precoated Ta and PS(ox) substrates, while cycloheximide treatment of the cells produced the same QCM-D response for fibronectin and serum precoatings on each of the materials. This confirmed that the process of matrix remodelling by the cells is dependent on the underlying substrate and the preadsorbed proteins and that the QCM-D response is dominated by changes in the underlying protein layer. Changes in dissipation correspond to the development of the actin cytoskeleton as visualised by actin staining.",
keywords = "Adsorption, Animals, Cattle, Cell Adhesion, Cell Culture Techniques, Cells, Cultured, Coated Materials, Biocompatible, Cycloheximide, Extracellular Matrix, Fibroblasts, Fibronectins, Mice, Microscopy, Fluorescence, NIH 3T3 Cells, Oxidation-Reduction, Polystyrenes, Protein Synthesis Inhibitors, Quartz, Serum, Serum Albumin, Bovine, Sheep, Substrate Specificity, Tantalum, Time Factors",
author = "Lord, {Megan S} and Charlotte Modin and Morten Foss and Mogens Duch and Anne Simmons and Pedersen, {Finn S} and Flemming Besenbacher and Milthorpe, {Bruce K}",
year = "2008",
doi = "10.1016/j.biomaterials.2008.03.002",
language = "English",
volume = "29",
pages = "2581--7",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "17",

}

RIS

TY - JOUR

T1 - Extracellular matrix remodelling during cell adhesion monitored by the quartz crystal microbalance

AU - Lord, Megan S

AU - Modin, Charlotte

AU - Foss, Morten

AU - Duch, Mogens

AU - Simmons, Anne

AU - Pedersen, Finn S

AU - Besenbacher, Flemming

AU - Milthorpe, Bruce K

PY - 2008

Y1 - 2008

N2 - A cell's ability to remodel adsorbed protein layers on surfaces is influenced by the nature of the protein layer itself. Remodelling is often required to accomplish cellular adhesion and extracellular matrix formation which forms the basis for cell spreading, increased adhesion and expression of different phenotypes. The adhesion of NIH3T3 (EGFP) fibroblasts to serum protein (albumin or fibronectin) precoated tantalum (Ta) and oxidised polystyrene (PS(ox)) surfaces was examined using the quartz crystal microbalance with dissipation (QCM-D) monitoring and fluorescence microscopy. The cells were either untreated or treated with cycloheximide to examine the contribution of endogenous protein production during cell adhesion to the QCM-D response over a period of 2h. Following adsorption of albumin onto Ta and PS(ox) there was no difference detected between the response to seeding untreated and cycloheximide treated cells. The QCM-D was able to detect differences in the untreated cellular responses to fibronectin versus serum precoated Ta and PS(ox) substrates, while cycloheximide treatment of the cells produced the same QCM-D response for fibronectin and serum precoatings on each of the materials. This confirmed that the process of matrix remodelling by the cells is dependent on the underlying substrate and the preadsorbed proteins and that the QCM-D response is dominated by changes in the underlying protein layer. Changes in dissipation correspond to the development of the actin cytoskeleton as visualised by actin staining.

AB - A cell's ability to remodel adsorbed protein layers on surfaces is influenced by the nature of the protein layer itself. Remodelling is often required to accomplish cellular adhesion and extracellular matrix formation which forms the basis for cell spreading, increased adhesion and expression of different phenotypes. The adhesion of NIH3T3 (EGFP) fibroblasts to serum protein (albumin or fibronectin) precoated tantalum (Ta) and oxidised polystyrene (PS(ox)) surfaces was examined using the quartz crystal microbalance with dissipation (QCM-D) monitoring and fluorescence microscopy. The cells were either untreated or treated with cycloheximide to examine the contribution of endogenous protein production during cell adhesion to the QCM-D response over a period of 2h. Following adsorption of albumin onto Ta and PS(ox) there was no difference detected between the response to seeding untreated and cycloheximide treated cells. The QCM-D was able to detect differences in the untreated cellular responses to fibronectin versus serum precoated Ta and PS(ox) substrates, while cycloheximide treatment of the cells produced the same QCM-D response for fibronectin and serum precoatings on each of the materials. This confirmed that the process of matrix remodelling by the cells is dependent on the underlying substrate and the preadsorbed proteins and that the QCM-D response is dominated by changes in the underlying protein layer. Changes in dissipation correspond to the development of the actin cytoskeleton as visualised by actin staining.

KW - Adsorption

KW - Animals

KW - Cattle

KW - Cell Adhesion

KW - Cell Culture Techniques

KW - Cells, Cultured

KW - Coated Materials, Biocompatible

KW - Cycloheximide

KW - Extracellular Matrix

KW - Fibroblasts

KW - Fibronectins

KW - Mice

KW - Microscopy, Fluorescence

KW - NIH 3T3 Cells

KW - Oxidation-Reduction

KW - Polystyrenes

KW - Protein Synthesis Inhibitors

KW - Quartz

KW - Serum

KW - Serum Albumin, Bovine

KW - Sheep

KW - Substrate Specificity

KW - Tantalum

KW - Time Factors

U2 - 10.1016/j.biomaterials.2008.03.002

DO - 10.1016/j.biomaterials.2008.03.002

M3 - Journal article

C2 - 18359077

VL - 29

SP - 2581

EP - 2587

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 17

ER -