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Expression of Fap amyloids in Pseudomonas aeruginosa, P. fluorescens, and P. putida results in aggregation and increased biofilm formation

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

Standard

Expression of Fap amyloids in Pseudomonas aeruginosa, P. fluorescens, and P. putida results in aggregation and increased biofilm formation. / Dueholm, Morten S; Søndergaard, Mads; Nilsson, Martin et al.

I: MicrobiologyOpen, Bind 2, Nr. 3, 06.2013, s. 365-382.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

Harvard

Dueholm, MS, Søndergaard, M, Nilsson, M, Christiansen, G, Stensballe, A, Overgaard, MT, Givskov, MC, Tolker-Nielsen, T, Otzen, D & Nielsen, PH 2013, 'Expression of Fap amyloids in Pseudomonas aeruginosa, P. fluorescens, and P. putida results in aggregation and increased biofilm formation', MicrobiologyOpen, bind 2, nr. 3, s. 365-382. https://doi.org/10.1002/mbo3.81

APA

Dueholm, M. S., Søndergaard, M., Nilsson, M., Christiansen, G., Stensballe, A., Overgaard, M. T., Givskov, M. C., Tolker-Nielsen, T., Otzen, D., & Nielsen, P. H. (2013). Expression of Fap amyloids in Pseudomonas aeruginosa, P. fluorescens, and P. putida results in aggregation and increased biofilm formation. MicrobiologyOpen, 2(3), 365-382. https://doi.org/10.1002/mbo3.81

CBE

Dueholm MS, Søndergaard M, Nilsson M, Christiansen G, Stensballe A, Overgaard MT, Givskov MC, Tolker-Nielsen T, Otzen D, Nielsen PH. 2013. Expression of Fap amyloids in Pseudomonas aeruginosa, P. fluorescens, and P. putida results in aggregation and increased biofilm formation. MicrobiologyOpen. 2(3):365-382. https://doi.org/10.1002/mbo3.81

MLA

Dueholm, Morten S et al. "Expression of Fap amyloids in Pseudomonas aeruginosa, P. fluorescens, and P. putida results in aggregation and increased biofilm formation". MicrobiologyOpen. 2013, 2(3). 365-382. https://doi.org/10.1002/mbo3.81

Vancouver

Dueholm MS, Søndergaard M, Nilsson M, Christiansen G, Stensballe A, Overgaard MT et al. Expression of Fap amyloids in Pseudomonas aeruginosa, P. fluorescens, and P. putida results in aggregation and increased biofilm formation. MicrobiologyOpen. 2013 jun.;2(3):365-382. doi: 10.1002/mbo3.81

Author

Dueholm, Morten S ; Søndergaard, Mads ; Nilsson, Martin et al. / Expression of Fap amyloids in Pseudomonas aeruginosa, P. fluorescens, and P. putida results in aggregation and increased biofilm formation. I: MicrobiologyOpen. 2013 ; Bind 2, Nr. 3. s. 365-382.

Bibtex

@article{0e228569ce594ed58076f7b77c68784c,
title = "Expression of Fap amyloids in Pseudomonas aeruginosa, P. fluorescens, and P. putida results in aggregation and increased biofilm formation",
abstract = "The fap operon, encoding functional amyloids in Pseudomonas (Fap), is present in most pseudomonads, but so far the expression and importance for biofilm formation has only been investigated for P. fluorescens strain UK4. In this study, we demonstrate the capacity of P. aeruginosa PAO1, P. fluorescens Pf-5, and P. putida F1 to express Fap fibrils, and investigated the effect of Fap expression on aggregation and biofilm formation. The fap operon in all three Pseudomonas species conferred the ability to express Fap fibrils as shown using a recombinant approach. This Fap overexpression consistently resulted in highly aggregative phenotypes and in increased biofilm formation. Detailed biophysical investigations of purified fibrils confirmed FapC as the main fibril monomer and supported the role of FapB as a minor, nucleating constituent as also indicated by bioinformatic analysis. Bioinformatics analysis suggested FapF and FapD as a potential β-barrel membrane pore and protease, respectively. Manipulation of the fap operon showed that FapA affects monomer composition of the final amyloid fibril, and that FapB is an amyloid protein, probably a nucleator for FapC polymerization. Our study highlights the fap operon as a molecular machine for functional amyloid formation.",
author = "Dueholm, {Morten S} and Mads S{\o}ndergaard and Martin Nilsson and Gunna Christiansen and Allan Stensballe and Overgaard, {Michael T} and Givskov, {Michael Christian} and Tim Tolker-Nielsen and Daniel Otzen and Nielsen, {Per H}",
note = "{\textcopyright} 2013 The Authors. Published by Blackwell Publishing Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.",
year = "2013",
month = jun,
doi = "10.1002/mbo3.81",
language = "English",
volume = "2",
pages = "365--382",
journal = "MicrobiologyOpen",
issn = "2045-8827",
publisher = "JohnWiley & Sons Ltd.",
number = "3",

}

RIS

TY - JOUR

T1 - Expression of Fap amyloids in Pseudomonas aeruginosa, P. fluorescens, and P. putida results in aggregation and increased biofilm formation

AU - Dueholm, Morten S

AU - Søndergaard, Mads

AU - Nilsson, Martin

AU - Christiansen, Gunna

AU - Stensballe, Allan

AU - Overgaard, Michael T

AU - Givskov, Michael Christian

AU - Tolker-Nielsen, Tim

AU - Otzen, Daniel

AU - Nielsen, Per H

N1 - © 2013 The Authors. Published by Blackwell Publishing Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PY - 2013/6

Y1 - 2013/6

N2 - The fap operon, encoding functional amyloids in Pseudomonas (Fap), is present in most pseudomonads, but so far the expression and importance for biofilm formation has only been investigated for P. fluorescens strain UK4. In this study, we demonstrate the capacity of P. aeruginosa PAO1, P. fluorescens Pf-5, and P. putida F1 to express Fap fibrils, and investigated the effect of Fap expression on aggregation and biofilm formation. The fap operon in all three Pseudomonas species conferred the ability to express Fap fibrils as shown using a recombinant approach. This Fap overexpression consistently resulted in highly aggregative phenotypes and in increased biofilm formation. Detailed biophysical investigations of purified fibrils confirmed FapC as the main fibril monomer and supported the role of FapB as a minor, nucleating constituent as also indicated by bioinformatic analysis. Bioinformatics analysis suggested FapF and FapD as a potential β-barrel membrane pore and protease, respectively. Manipulation of the fap operon showed that FapA affects monomer composition of the final amyloid fibril, and that FapB is an amyloid protein, probably a nucleator for FapC polymerization. Our study highlights the fap operon as a molecular machine for functional amyloid formation.

AB - The fap operon, encoding functional amyloids in Pseudomonas (Fap), is present in most pseudomonads, but so far the expression and importance for biofilm formation has only been investigated for P. fluorescens strain UK4. In this study, we demonstrate the capacity of P. aeruginosa PAO1, P. fluorescens Pf-5, and P. putida F1 to express Fap fibrils, and investigated the effect of Fap expression on aggregation and biofilm formation. The fap operon in all three Pseudomonas species conferred the ability to express Fap fibrils as shown using a recombinant approach. This Fap overexpression consistently resulted in highly aggregative phenotypes and in increased biofilm formation. Detailed biophysical investigations of purified fibrils confirmed FapC as the main fibril monomer and supported the role of FapB as a minor, nucleating constituent as also indicated by bioinformatic analysis. Bioinformatics analysis suggested FapF and FapD as a potential β-barrel membrane pore and protease, respectively. Manipulation of the fap operon showed that FapA affects monomer composition of the final amyloid fibril, and that FapB is an amyloid protein, probably a nucleator for FapC polymerization. Our study highlights the fap operon as a molecular machine for functional amyloid formation.

U2 - 10.1002/mbo3.81

DO - 10.1002/mbo3.81

M3 - Journal article

C2 - 23504942

VL - 2

SP - 365

EP - 382

JO - MicrobiologyOpen

JF - MicrobiologyOpen

SN - 2045-8827

IS - 3

ER -