Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)

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  • Bruno Studer, Danmark
  • Torben Asp
  • Ursula Frei, Danmark
  • Stephan Hentrup
  • Helena Meally, Crops Research Centre Oak Park, Irland
  • Aurélie Guillard, Crops Research Centre Oak Park, Irland
  • Susanne Barth, Crops Research Centre Oak Park, Irland
  • Hilde Muylle, Plant Unit, Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research (ILVO), Belgien
  • Isabel Roldán-Ruiz, Plant Unit, Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research (ILVO), Belgien
  • Philippe Barre, Unité de Génétique et d’Amélioration des Plantes Fourragères, Institut National de Recherche Agronomique (INRA), Frankrig
  • Carole Koning-Boucoiran, Plant Research International B.V. (PRI), Holland
  • Gerda Uenk-Stunnenberg, Plant Research International B.V. (PRI), Holland
  • Oene Dolstra, Plant Research International B.V. (PRI), Holland
  • Leif Skøt, Plant Genetics and Breeding Department, Institute of Grassland and Environmental Research (IGER), Storbritannien
  • Kirsten P Skøt, Plant Genetics and Breeding Department, Institute of Grassland and Environmental Research (IGER), Storbritannien
  • Lesley B Turner, Plant Genetics and Breeding Department, Institute of Grassland and Environmental Research (IGER), Storbritannien
  • Mervyn O Humphreys, Plant Genetics and Breeding Department, Institute of Grassland and Environmental Research (IGER), Storbritannien
  • Roland Kölliker, Agroscope Reckenholz-Tänikon Research Station (ART), Schweiz
  • Niels Roulund, DLF-Trifolium, Dansk Planteforædling, Danmark
  • Klaus K Nielsen, DLF-Trifolium, Dansk Planteforædling, Danmark
  • Thomas Lübberstedt, Danmark
  • Institut for Genetik og Bioteknologi
  • Molekylær Genetik og Bioteknologi
An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing ESTs, 744 were used for primer design. Primer amplification was tested in eight genotypes of L. perenne and L. multiflorum representing (grand-) parents of four mapping populations and resulted in 464 successfully amplified EST-SSRs. Three hundred and six primer pairs successfully amplified products in the mapping population VrnA derived from two of the eight genotypes included in the original screening and revealed SSR polymorphisms for 143 ESTs. Here, we report on 464 EST-derived SSR primer sequences of perennial ryegrass established in laboratory assays, providing a dedicated tool for marker assisted breeding and comparative mapping within and among forage and turf grasses.
Udgivelsesdato: May
OriginalsprogEngelsk
TidsskriftMolecular Breeding
Vol/bind21
Nummer4
Sider (fra-til)533-548
Antal sider16
ISSN1380-3743
DOI
StatusUdgivet - 2008

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