TY - JOUR
T1 - Evolution and regulation of the Lotus japonicus LysM receptor gene family
AU - Lohmann, Gitte Vestergaard
AU - Shimoda, Yoshikazu
AU - Nielsen, Mette Wibroe
AU - Jørgensen, Frank Grønlund
AU - Grossmann, Christina
AU - Sandal, Niels
AU - Sørensen, Kirsten
AU - Thirup, Søren
AU - Madsen, Lene Heegaard
AU - Tabata, Satoshi
AU - Sato, Shusei
AU - Stougaard, Jens
AU - Radutoiu, Simona
PY - 2010
Y1 - 2010
N2 - LysM receptor kinases were identified as receptors of acylated chitin (Nod factors) or chitin produced by plant-interacting microbes. Here, we present the identification and characterization of the LysM receptor kinase gene (Lys) family (17 members) in Lotus japonicus. Comprehensive phylogenetic analysis revealed a correlation between Lys gene structure and phylogeny. Further mapping coupled with sequence-based anchoring on the genome showed that the family has probably expanded by a combination of tandem and segmental duplication events. Using a sliding-window approach, we identified distinct regions in the LysM and kinase domains of recently diverged Lys genes where positive selection may have shaped ligand interaction. Interestingly, in the case of NFR5 and its closest paralog, LYS11, one of these regions coincides with the predicted Nod-factor binding groove and the suggested specificity determining area of the second LysM domain. One hypothesis for the evolutionary diversification of this receptor family in legumes is their unique capacity to decipher various structures of chitin-derived molecules produced by an extended spectrum of interacting organisms: symbiotic, associative, endophytic, and parasitic. In a detailed expression analysis, we found several Lotus Lys genes regulated not only during the symbiotic association with Mesorhizobium loti but also in response to chitin treatment.
AB - LysM receptor kinases were identified as receptors of acylated chitin (Nod factors) or chitin produced by plant-interacting microbes. Here, we present the identification and characterization of the LysM receptor kinase gene (Lys) family (17 members) in Lotus japonicus. Comprehensive phylogenetic analysis revealed a correlation between Lys gene structure and phylogeny. Further mapping coupled with sequence-based anchoring on the genome showed that the family has probably expanded by a combination of tandem and segmental duplication events. Using a sliding-window approach, we identified distinct regions in the LysM and kinase domains of recently diverged Lys genes where positive selection may have shaped ligand interaction. Interestingly, in the case of NFR5 and its closest paralog, LYS11, one of these regions coincides with the predicted Nod-factor binding groove and the suggested specificity determining area of the second LysM domain. One hypothesis for the evolutionary diversification of this receptor family in legumes is their unique capacity to decipher various structures of chitin-derived molecules produced by an extended spectrum of interacting organisms: symbiotic, associative, endophytic, and parasitic. In a detailed expression analysis, we found several Lotus Lys genes regulated not only during the symbiotic association with Mesorhizobium loti but also in response to chitin treatment.
KW - Base Sequence
KW - Chromosome Mapping
KW - Chromosomes, Plant
KW - Evolution, Molecular
KW - Lotus
KW - Molecular Sequence Data
KW - Multigene Family
KW - Phylogeny
KW - Plant Proteins
KW - Sequence Alignment
U2 - 10.1094/MPMI-23-4-0510
DO - 10.1094/MPMI-23-4-0510
M3 - Journal article
C2 - 20192837
SN - 0894-0282
VL - 23
SP - 510
EP - 521
JO - Molecular plant-microbe interactions
JF - Molecular plant-microbe interactions
IS - 4
ER -