Evaluation of Simple and Inexpensive High-Throughput Methods for Phytic Acid Determination

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Standard

Evaluation of Simple and Inexpensive High-Throughput Methods for Phytic Acid Determination. / Raboy, Victor; Johnson, Amy; Bilyeu, Kristin; Brinch-Pedersen, Henrik; Cichy, Karen; Hurrell, Richard F; Zeder, Christophe; Rasmussen, Søren Kjærsgaard; Warkentin, Tom D; Thavarajah, Pushparajah; Shi, Jinrui ; Zhou, Lan; Shu, Qingyao .

I: Journal of the American Oil Chemists' Society, Bind 94, Nr. 3, 03.2017, s. 353-362.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Raboy, V, Johnson, A, Bilyeu, K, Brinch-Pedersen, H, Cichy, K, Hurrell, RF, Zeder, C, Rasmussen, SK, Warkentin, TD, Thavarajah, P, Shi, J, Zhou, L & Shu, Q 2017, 'Evaluation of Simple and Inexpensive High-Throughput Methods for Phytic Acid Determination', Journal of the American Oil Chemists' Society, bind 94, nr. 3, s. 353-362. https://doi.org/10.1007/s11746-016-2946-y

APA

Raboy, V., Johnson, A., Bilyeu, K., Brinch-Pedersen, H., Cichy, K., Hurrell, R. F., ... Shu, Q. (2017). Evaluation of Simple and Inexpensive High-Throughput Methods for Phytic Acid Determination. Journal of the American Oil Chemists' Society, 94(3), 353-362. https://doi.org/10.1007/s11746-016-2946-y

CBE

Raboy V, Johnson A, Bilyeu K, Brinch-Pedersen H, Cichy K, Hurrell RF, Zeder C, Rasmussen SK, Warkentin TD, Thavarajah P, Shi J, Zhou L, Shu Q. 2017. Evaluation of Simple and Inexpensive High-Throughput Methods for Phytic Acid Determination. Journal of the American Oil Chemists' Society. 94(3):353-362. https://doi.org/10.1007/s11746-016-2946-y

MLA

Raboy, Victor o.a.. "Evaluation of Simple and Inexpensive High-Throughput Methods for Phytic Acid Determination". Journal of the American Oil Chemists' Society. 2017, 94(3). 353-362. https://doi.org/10.1007/s11746-016-2946-y

Vancouver

Raboy V, Johnson A, Bilyeu K, Brinch-Pedersen H, Cichy K, Hurrell RF o.a. Evaluation of Simple and Inexpensive High-Throughput Methods for Phytic Acid Determination. Journal of the American Oil Chemists' Society. 2017 mar;94(3):353-362. https://doi.org/10.1007/s11746-016-2946-y

Author

Raboy, Victor ; Johnson, Amy ; Bilyeu, Kristin ; Brinch-Pedersen, Henrik ; Cichy, Karen ; Hurrell, Richard F ; Zeder, Christophe ; Rasmussen, Søren Kjærsgaard ; Warkentin, Tom D ; Thavarajah, Pushparajah ; Shi, Jinrui ; Zhou, Lan ; Shu, Qingyao . / Evaluation of Simple and Inexpensive High-Throughput Methods for Phytic Acid Determination. I: Journal of the American Oil Chemists' Society. 2017 ; Bind 94, Nr. 3. s. 353-362.

Bibtex

@article{53f7ea7cd21449cdb0fe1366943daef7,
title = "Evaluation of Simple and Inexpensive High-Throughput Methods for Phytic Acid Determination",
abstract = "High-throughput/low-cost/low-tech methods for phytic acid determination that are sufficiently accurate and reproducible would be of value in plant genetics, crop breeding and in the food and feed industries. Variants of two candidate methods, those described by Vaintraub and Lapteva (Anal Biochem 175:227–24, 1988; “VL” methods) and Huang and Lantzsch (J Sci Food Agric 34:1423–1426, 1983; “HL” methods), were evaluated. The primary concern with these methods is that, due to interference of matrix constituents including inorganic P, they can overestimate phytic acid and are ineffective at low levels of phytic acid. Twelve seed flours, representing lines of soybean, maize, barley and dry bean, containing a wide range of phytic acid levels, were analyzed by a minimum of eight cooperating laboratories using three variants of the VL method and two variants of the HL method. No method had consistently acceptable (˂2.0”) “Horwitz ratios”, a measure of reproducibility, although some treatments approached that. For example, one variant of the VL method when used to assay a soybean flour with a “standard” level of phytic acid had a Horwitz ratio of 2.15. Some variants of the VL method were adequate for analyses of cereal grains regardless of phytic acid level but none accurately measured phytic acid when at low levels in soybean flours. One variant of the HL method in which the 0.2 N HCl extraction media is modified to contain 10{\%} Na2SO4, did accurately measure phytic acid levels in both cereal and legume flours regardless of endogenous phytic acid levels or matrix constituents.",
keywords = "Phytic acid determination, myo-inositol hexaphosphate, Seed flour, Soybean , Maize , Barley, Dry bean",
author = "Victor Raboy and Amy Johnson and Kristin Bilyeu and Henrik Brinch-Pedersen and Karen Cichy and Hurrell, {Richard F} and Christophe Zeder and Rasmussen, {S{\o}ren Kj{\ae}rsgaard} and Warkentin, {Tom D} and Pushparajah Thavarajah and Jinrui Shi and Lan Zhou and Qingyao Shu",
year = "2017",
month = "3",
doi = "10.1007/s11746-016-2946-y",
language = "English",
volume = "94",
pages = "353--362",
journal = "Journal of the American Oil Chemists' Society",
issn = "0003-021X",
publisher = "Springer",
number = "3",

}

RIS

TY - JOUR

T1 - Evaluation of Simple and Inexpensive High-Throughput Methods for Phytic Acid Determination

AU - Raboy, Victor

AU - Johnson, Amy

AU - Bilyeu, Kristin

AU - Brinch-Pedersen, Henrik

AU - Cichy, Karen

AU - Hurrell, Richard F

AU - Zeder, Christophe

AU - Rasmussen, Søren Kjærsgaard

AU - Warkentin, Tom D

AU - Thavarajah, Pushparajah

AU - Shi, Jinrui

AU - Zhou, Lan

AU - Shu, Qingyao

PY - 2017/3

Y1 - 2017/3

N2 - High-throughput/low-cost/low-tech methods for phytic acid determination that are sufficiently accurate and reproducible would be of value in plant genetics, crop breeding and in the food and feed industries. Variants of two candidate methods, those described by Vaintraub and Lapteva (Anal Biochem 175:227–24, 1988; “VL” methods) and Huang and Lantzsch (J Sci Food Agric 34:1423–1426, 1983; “HL” methods), were evaluated. The primary concern with these methods is that, due to interference of matrix constituents including inorganic P, they can overestimate phytic acid and are ineffective at low levels of phytic acid. Twelve seed flours, representing lines of soybean, maize, barley and dry bean, containing a wide range of phytic acid levels, were analyzed by a minimum of eight cooperating laboratories using three variants of the VL method and two variants of the HL method. No method had consistently acceptable (˂2.0”) “Horwitz ratios”, a measure of reproducibility, although some treatments approached that. For example, one variant of the VL method when used to assay a soybean flour with a “standard” level of phytic acid had a Horwitz ratio of 2.15. Some variants of the VL method were adequate for analyses of cereal grains regardless of phytic acid level but none accurately measured phytic acid when at low levels in soybean flours. One variant of the HL method in which the 0.2 N HCl extraction media is modified to contain 10% Na2SO4, did accurately measure phytic acid levels in both cereal and legume flours regardless of endogenous phytic acid levels or matrix constituents.

AB - High-throughput/low-cost/low-tech methods for phytic acid determination that are sufficiently accurate and reproducible would be of value in plant genetics, crop breeding and in the food and feed industries. Variants of two candidate methods, those described by Vaintraub and Lapteva (Anal Biochem 175:227–24, 1988; “VL” methods) and Huang and Lantzsch (J Sci Food Agric 34:1423–1426, 1983; “HL” methods), were evaluated. The primary concern with these methods is that, due to interference of matrix constituents including inorganic P, they can overestimate phytic acid and are ineffective at low levels of phytic acid. Twelve seed flours, representing lines of soybean, maize, barley and dry bean, containing a wide range of phytic acid levels, were analyzed by a minimum of eight cooperating laboratories using three variants of the VL method and two variants of the HL method. No method had consistently acceptable (˂2.0”) “Horwitz ratios”, a measure of reproducibility, although some treatments approached that. For example, one variant of the VL method when used to assay a soybean flour with a “standard” level of phytic acid had a Horwitz ratio of 2.15. Some variants of the VL method were adequate for analyses of cereal grains regardless of phytic acid level but none accurately measured phytic acid when at low levels in soybean flours. One variant of the HL method in which the 0.2 N HCl extraction media is modified to contain 10% Na2SO4, did accurately measure phytic acid levels in both cereal and legume flours regardless of endogenous phytic acid levels or matrix constituents.

KW - Phytic acid determination

KW - myo-inositol hexaphosphate

KW - Seed flour

KW - Soybean

KW - Maize

KW - Barley

KW - Dry bean

U2 - 10.1007/s11746-016-2946-y

DO - 10.1007/s11746-016-2946-y

M3 - Journal article

VL - 94

SP - 353

EP - 362

JO - Journal of the American Oil Chemists' Society

JF - Journal of the American Oil Chemists' Society

SN - 0003-021X

IS - 3

ER -