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Evaluation of miR-302 promoter activity in transgenic mice and pluripotent stem cell lines

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Evaluation of miR-302 promoter activity in transgenic mice and pluripotent stem cell lines. / Rahimi, Karim; Parsa, Sara; Nikzaban, Mehrnoush; Khaledian, Behnoush; Mowla, Seyed Javad; Fathi, Fardin.

I: In Vitro Cellular and Developmental Biology - Animal, Bind 56, Nr. 10, 12.2020, s. 896-905.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Rahimi, K, Parsa, S, Nikzaban, M, Khaledian, B, Mowla, SJ & Fathi, F 2020, 'Evaluation of miR-302 promoter activity in transgenic mice and pluripotent stem cell lines', In Vitro Cellular and Developmental Biology - Animal, bind 56, nr. 10, s. 896-905. https://doi.org/10.1007/s11626-020-00516-8

APA

Rahimi, K., Parsa, S., Nikzaban, M., Khaledian, B., Mowla, S. J., & Fathi, F. (2020). Evaluation of miR-302 promoter activity in transgenic mice and pluripotent stem cell lines. In Vitro Cellular and Developmental Biology - Animal, 56(10), 896-905. https://doi.org/10.1007/s11626-020-00516-8

CBE

Rahimi K, Parsa S, Nikzaban M, Khaledian B, Mowla SJ, Fathi F. 2020. Evaluation of miR-302 promoter activity in transgenic mice and pluripotent stem cell lines. In Vitro Cellular and Developmental Biology - Animal. 56(10):896-905. https://doi.org/10.1007/s11626-020-00516-8

MLA

Vancouver

Rahimi K, Parsa S, Nikzaban M, Khaledian B, Mowla SJ, Fathi F. Evaluation of miR-302 promoter activity in transgenic mice and pluripotent stem cell lines. In Vitro Cellular and Developmental Biology - Animal. 2020 dec;56(10):896-905. https://doi.org/10.1007/s11626-020-00516-8

Author

Rahimi, Karim ; Parsa, Sara ; Nikzaban, Mehrnoush ; Khaledian, Behnoush ; Mowla, Seyed Javad ; Fathi, Fardin. / Evaluation of miR-302 promoter activity in transgenic mice and pluripotent stem cell lines. I: In Vitro Cellular and Developmental Biology - Animal. 2020 ; Bind 56, Nr. 10. s. 896-905.

Bibtex

@article{ea55bb379eee4081bdd0be8e8c34f8b8,
title = "Evaluation of miR-302 promoter activity in transgenic mice and pluripotent stem cell lines",
abstract = "Some miRNAs, including the miR-302 cluster, are critical regulators of the stemness state of embryonic stem cells and cell fate patterning. In this study, we evaluated the activity of the miR-302 core promotor in mice and human pluripotent stem cells, somatic tissue derivatives, and generated transgenic mice expressing EGFP under a miR-302 promoter. The expression of EGFP under the control of the miR-302 promotor was examined in the cell lines and somatic tissues of transgenic mice, transgenic blastocysts, and embryonic stem cells derived from transgenic blastocysts. Our results showed that the miR-302 promoter is highly expressed in the mouse and human pluripotent cells, weakly expressed in the somatic tissue derivatives, is highly expressed in both blastocysts and the first passages of transgenic embryonic stem cells, and lowly expressed in the somatic tissues of transgenic mice. It can be concluded that different temporal and spatial gene expression patterns occur during the embryonic and adult stages of cells in mice.",
keywords = "Adult stem cells, Blastocyst, Embryonic stem cells, MicroRNAs, Transgenic mice",
author = "Karim Rahimi and Sara Parsa and Mehrnoush Nikzaban and Behnoush Khaledian and Mowla, {Seyed Javad} and Fardin Fathi",
note = "Publisher Copyright: {\textcopyright} 2020, The Society for In Vitro Biology. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2020",
month = dec,
doi = "10.1007/s11626-020-00516-8",
language = "English",
volume = "56",
pages = "896--905",
journal = "In Vitro Cellular & Developmental Biology - Animal",
issn = "1071-2690",
publisher = "Springer",
number = "10",

}

RIS

TY - JOUR

T1 - Evaluation of miR-302 promoter activity in transgenic mice and pluripotent stem cell lines

AU - Rahimi, Karim

AU - Parsa, Sara

AU - Nikzaban, Mehrnoush

AU - Khaledian, Behnoush

AU - Mowla, Seyed Javad

AU - Fathi, Fardin

N1 - Publisher Copyright: © 2020, The Society for In Vitro Biology. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/12

Y1 - 2020/12

N2 - Some miRNAs, including the miR-302 cluster, are critical regulators of the stemness state of embryonic stem cells and cell fate patterning. In this study, we evaluated the activity of the miR-302 core promotor in mice and human pluripotent stem cells, somatic tissue derivatives, and generated transgenic mice expressing EGFP under a miR-302 promoter. The expression of EGFP under the control of the miR-302 promotor was examined in the cell lines and somatic tissues of transgenic mice, transgenic blastocysts, and embryonic stem cells derived from transgenic blastocysts. Our results showed that the miR-302 promoter is highly expressed in the mouse and human pluripotent cells, weakly expressed in the somatic tissue derivatives, is highly expressed in both blastocysts and the first passages of transgenic embryonic stem cells, and lowly expressed in the somatic tissues of transgenic mice. It can be concluded that different temporal and spatial gene expression patterns occur during the embryonic and adult stages of cells in mice.

AB - Some miRNAs, including the miR-302 cluster, are critical regulators of the stemness state of embryonic stem cells and cell fate patterning. In this study, we evaluated the activity of the miR-302 core promotor in mice and human pluripotent stem cells, somatic tissue derivatives, and generated transgenic mice expressing EGFP under a miR-302 promoter. The expression of EGFP under the control of the miR-302 promotor was examined in the cell lines and somatic tissues of transgenic mice, transgenic blastocysts, and embryonic stem cells derived from transgenic blastocysts. Our results showed that the miR-302 promoter is highly expressed in the mouse and human pluripotent cells, weakly expressed in the somatic tissue derivatives, is highly expressed in both blastocysts and the first passages of transgenic embryonic stem cells, and lowly expressed in the somatic tissues of transgenic mice. It can be concluded that different temporal and spatial gene expression patterns occur during the embryonic and adult stages of cells in mice.

KW - Adult stem cells

KW - Blastocyst

KW - Embryonic stem cells

KW - MicroRNAs

KW - Transgenic mice

UR - http://www.scopus.com/inward/record.url?scp=85096301396&partnerID=8YFLogxK

U2 - 10.1007/s11626-020-00516-8

DO - 10.1007/s11626-020-00516-8

M3 - Journal article

C2 - 33210246

AN - SCOPUS:85096301396

VL - 56

SP - 896

EP - 905

JO - In Vitro Cellular & Developmental Biology - Animal

JF - In Vitro Cellular & Developmental Biology - Animal

SN - 1071-2690

IS - 10

ER -