Evaluation of Ebselen-azadioxatriangulenium as redox-sensitive fluorescent intracellular probe and as indicator within a planar redox optode

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Evaluation of Ebselen-azadioxatriangulenium as redox-sensitive fluorescent intracellular probe and as indicator within a planar redox optode. / Koren, Klaus; Gravesen Salinas, Nina Katharina; Santella, Marco; Moßhammer, Maria; Müller, Marie Caroline; Dmitriev, Ruslan I.; Borisov, Sergey M.; Kühl, Michael; Laursen, Bo W.

I: Dyes and Pigments, Bind 173, 107866, 2020.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Koren, K, Gravesen Salinas, NK, Santella, M, Moßhammer, M, Müller, MC, Dmitriev, RI, Borisov, SM, Kühl, M & Laursen, BW 2020, 'Evaluation of Ebselen-azadioxatriangulenium as redox-sensitive fluorescent intracellular probe and as indicator within a planar redox optode', Dyes and Pigments, bind 173, 107866. https://doi.org/10.1016/j.dyepig.2019.107866

APA

Koren, K., Gravesen Salinas, N. K., Santella, M., Moßhammer, M., Müller, M. C., Dmitriev, R. I., Borisov, S. M., Kühl, M., & Laursen, B. W. (2020). Evaluation of Ebselen-azadioxatriangulenium as redox-sensitive fluorescent intracellular probe and as indicator within a planar redox optode. Dyes and Pigments, 173, [107866]. https://doi.org/10.1016/j.dyepig.2019.107866

CBE

Koren K, Gravesen Salinas NK, Santella M, Moßhammer M, Müller MC, Dmitriev RI, Borisov SM, Kühl M, Laursen BW. 2020. Evaluation of Ebselen-azadioxatriangulenium as redox-sensitive fluorescent intracellular probe and as indicator within a planar redox optode. Dyes and Pigments. 173:Article 107866. https://doi.org/10.1016/j.dyepig.2019.107866

MLA

Vancouver

Author

Koren, Klaus ; Gravesen Salinas, Nina Katharina ; Santella, Marco ; Moßhammer, Maria ; Müller, Marie Caroline ; Dmitriev, Ruslan I. ; Borisov, Sergey M. ; Kühl, Michael ; Laursen, Bo W. / Evaluation of Ebselen-azadioxatriangulenium as redox-sensitive fluorescent intracellular probe and as indicator within a planar redox optode. I: Dyes and Pigments. 2020 ; Bind 173.

Bibtex

@article{6a14378eab204cdaa2c3f52c09142f2f,
title = "Evaluation of Ebselen-azadioxatriangulenium as redox-sensitive fluorescent intracellular probe and as indicator within a planar redox optode",
abstract = "Redox homeostasis is essential for cellular survival and is therefore tightly regulated. Visualizing changes in the local redox state is essential for detecting oxidative stress, especially with the help of red, long emission lifetime probes. We synthesized a new reversible, redox-sensitive fluorescent probe, ebselen-azadioxatriangulenium (Ebselen-ADOTA), and characterized its potential use for intra- and extracellular redox sensing. The probe consists of a photostable triangulenium-based fluorophore (emission maximum at 564 nm) modulated by a redox-active ebselen motif. The reduced form only shows around 1/10 of the emission intensity of the oxidized form. The Ebselen-ADOTA probe exhibited reversible oxidation and reduction in solution under extracellular conditions. In addition, Ebselen-ADOTA can visualize an increase in oxidative stress within anaerobic bacteria when exposing them to air, the probe did not readily respond to changes in the redox environment inside mammalian cells, probably due to unfavorable localization, aggregation or binding to cysteine containing proteins. When incorporated inside a polymer matrix (hydrogel D4 mixed with poly(acrylic acid)), the probe showed a rapid and fully reversible response to redox changes, thus enabling the preparation of a redox-sensitive optode.",
keywords = "Fluorescence, Indicator, Optical sensor, Redox chemistry, Triangulenium",
author = "Klaus Koren and {Gravesen Salinas}, {Nina Katharina} and Marco Santella and Maria Mo{\ss}hammer and M{\"u}ller, {Marie Caroline} and Dmitriev, {Ruslan I.} and Borisov, {Sergey M.} and Michael K{\"u}hl and Laursen, {Bo W.}",
year = "2020",
doi = "10.1016/j.dyepig.2019.107866",
language = "English",
volume = "173",
journal = "Dyes and Pigments",
issn = "0143-7208",
publisher = "Pergamon Press",

}

RIS

TY - JOUR

T1 - Evaluation of Ebselen-azadioxatriangulenium as redox-sensitive fluorescent intracellular probe and as indicator within a planar redox optode

AU - Koren, Klaus

AU - Gravesen Salinas, Nina Katharina

AU - Santella, Marco

AU - Moßhammer, Maria

AU - Müller, Marie Caroline

AU - Dmitriev, Ruslan I.

AU - Borisov, Sergey M.

AU - Kühl, Michael

AU - Laursen, Bo W.

PY - 2020

Y1 - 2020

N2 - Redox homeostasis is essential for cellular survival and is therefore tightly regulated. Visualizing changes in the local redox state is essential for detecting oxidative stress, especially with the help of red, long emission lifetime probes. We synthesized a new reversible, redox-sensitive fluorescent probe, ebselen-azadioxatriangulenium (Ebselen-ADOTA), and characterized its potential use for intra- and extracellular redox sensing. The probe consists of a photostable triangulenium-based fluorophore (emission maximum at 564 nm) modulated by a redox-active ebselen motif. The reduced form only shows around 1/10 of the emission intensity of the oxidized form. The Ebselen-ADOTA probe exhibited reversible oxidation and reduction in solution under extracellular conditions. In addition, Ebselen-ADOTA can visualize an increase in oxidative stress within anaerobic bacteria when exposing them to air, the probe did not readily respond to changes in the redox environment inside mammalian cells, probably due to unfavorable localization, aggregation or binding to cysteine containing proteins. When incorporated inside a polymer matrix (hydrogel D4 mixed with poly(acrylic acid)), the probe showed a rapid and fully reversible response to redox changes, thus enabling the preparation of a redox-sensitive optode.

AB - Redox homeostasis is essential for cellular survival and is therefore tightly regulated. Visualizing changes in the local redox state is essential for detecting oxidative stress, especially with the help of red, long emission lifetime probes. We synthesized a new reversible, redox-sensitive fluorescent probe, ebselen-azadioxatriangulenium (Ebselen-ADOTA), and characterized its potential use for intra- and extracellular redox sensing. The probe consists of a photostable triangulenium-based fluorophore (emission maximum at 564 nm) modulated by a redox-active ebselen motif. The reduced form only shows around 1/10 of the emission intensity of the oxidized form. The Ebselen-ADOTA probe exhibited reversible oxidation and reduction in solution under extracellular conditions. In addition, Ebselen-ADOTA can visualize an increase in oxidative stress within anaerobic bacteria when exposing them to air, the probe did not readily respond to changes in the redox environment inside mammalian cells, probably due to unfavorable localization, aggregation or binding to cysteine containing proteins. When incorporated inside a polymer matrix (hydrogel D4 mixed with poly(acrylic acid)), the probe showed a rapid and fully reversible response to redox changes, thus enabling the preparation of a redox-sensitive optode.

KW - Fluorescence

KW - Indicator

KW - Optical sensor

KW - Redox chemistry

KW - Triangulenium

UR - http://www.scopus.com/inward/record.url?scp=85073725540&partnerID=8YFLogxK

U2 - 10.1016/j.dyepig.2019.107866

DO - 10.1016/j.dyepig.2019.107866

M3 - Journal article

AN - SCOPUS:85073725540

VL - 173

JO - Dyes and Pigments

JF - Dyes and Pigments

SN - 0143-7208

M1 - 107866

ER -