Estimating the wound healing ability of bioactive milk proteins using an optimized cell based assay.

Publikation: KonferencebidragPosterForskning

Standard

Estimating the wound healing ability of bioactive milk proteins using an optimized cell based assay. / Nyegaard, Steffen; Andreasen, Trine; Rasmussen, Jan Trige.

2012. Poster session præsenteret ved 9th International Symposium on Milk Genomics & Human Health, Wageningen, Holland.

Publikation: KonferencebidragPosterForskning

Harvard

Nyegaard, S, Andreasen, T & Rasmussen, JT 2012, 'Estimating the wound healing ability of bioactive milk proteins using an optimized cell based assay.', 9th International Symposium on Milk Genomics & Human Health, Wageningen, Holland, 08/10/2012 - 10/10/2012.

APA

Nyegaard, S., Andreasen, T., & Rasmussen, J. T. (2012). Estimating the wound healing ability of bioactive milk proteins using an optimized cell based assay.. Poster session præsenteret ved 9th International Symposium on Milk Genomics & Human Health, Wageningen, Holland.

CBE

Nyegaard S, Andreasen T, Rasmussen JT. 2012. Estimating the wound healing ability of bioactive milk proteins using an optimized cell based assay. Poster session præsenteret ved 9th International Symposium on Milk Genomics & Human Health, Wageningen, Holland.

MLA

Nyegaard, Steffen, Trine Andreasen, og Jan Trige Rasmussen Estimating the wound healing ability of bioactive milk proteins using an optimized cell based assay.. 9th International Symposium on Milk Genomics & Human Health, 08 okt. 2012, Wageningen, Holland, Poster, 2012. 1 s.

Vancouver

Nyegaard S, Andreasen T, Rasmussen JT. Estimating the wound healing ability of bioactive milk proteins using an optimized cell based assay.. 2012. Poster session præsenteret ved 9th International Symposium on Milk Genomics & Human Health, Wageningen, Holland.

Author

Nyegaard, Steffen ; Andreasen, Trine ; Rasmussen, Jan Trige. / Estimating the wound healing ability of bioactive milk proteins using an optimized cell based assay. Poster session præsenteret ved 9th International Symposium on Milk Genomics & Human Health, Wageningen, Holland.1 s.

Bibtex

@conference{77c9339709eb4769a35b9315871c762e,
title = "Estimating the wound healing ability of bioactive milk proteins using an optimized cell based assay.",
abstract = "Milk contains many different proteins of which the larger constituents like the caseins and major whey constituents are well characterized. We have for some time been studying the structure and function of proteins associated with the milk fat globule membrane like lactadherin, MUC1/15, xanthineoxidoreductase along with minor whey constituents like osteopontin, EPV20 etc. The enterocyte migration rate is a key parameter in maintaining intestinal homeostasis and intestinal repair when recovering from infection or intestinal diseases like Crohns and ulcerative colitis. We developed a novel in vitro wound healing assay to determine the bioactive effects of various milk proteins using human small intestine cells grown on extracellular matrix. Silicone inserts are placed in a 96-well plate and enterocytes seeded around it, creating a monolayer with a cell free area. In current ongoing experiments, various pure milk proteins and isolates are added and migration quantified. This is done by performing a nuclei count and by measuring the migrated distance. The high reproducibility and gentle nature of the inserts makes this approach a good alternative to the traditional scratch assay. In perspective, it will of course be interesting to see if there are new applications for isolated bioactive milk proteins or industrial derived milk protein ingredients. ",
keywords = "Cellemigration, S{\aa}rheling, Bioaktivitet",
author = "Steffen Nyegaard and Trine Andreasen and Rasmussen, {Jan Trige}",
year = "2012",
month = oct,
day = "8",
language = "Dansk",
note = "null ; Conference date: 08-10-2012 Through 10-10-2012",

}

RIS

TY - CONF

T1 - Estimating the wound healing ability of bioactive milk proteins using an optimized cell based assay.

AU - Nyegaard, Steffen

AU - Andreasen, Trine

AU - Rasmussen, Jan Trige

N1 - Conference code: 9

PY - 2012/10/8

Y1 - 2012/10/8

N2 - Milk contains many different proteins of which the larger constituents like the caseins and major whey constituents are well characterized. We have for some time been studying the structure and function of proteins associated with the milk fat globule membrane like lactadherin, MUC1/15, xanthineoxidoreductase along with minor whey constituents like osteopontin, EPV20 etc. The enterocyte migration rate is a key parameter in maintaining intestinal homeostasis and intestinal repair when recovering from infection or intestinal diseases like Crohns and ulcerative colitis. We developed a novel in vitro wound healing assay to determine the bioactive effects of various milk proteins using human small intestine cells grown on extracellular matrix. Silicone inserts are placed in a 96-well plate and enterocytes seeded around it, creating a monolayer with a cell free area. In current ongoing experiments, various pure milk proteins and isolates are added and migration quantified. This is done by performing a nuclei count and by measuring the migrated distance. The high reproducibility and gentle nature of the inserts makes this approach a good alternative to the traditional scratch assay. In perspective, it will of course be interesting to see if there are new applications for isolated bioactive milk proteins or industrial derived milk protein ingredients.

AB - Milk contains many different proteins of which the larger constituents like the caseins and major whey constituents are well characterized. We have for some time been studying the structure and function of proteins associated with the milk fat globule membrane like lactadherin, MUC1/15, xanthineoxidoreductase along with minor whey constituents like osteopontin, EPV20 etc. The enterocyte migration rate is a key parameter in maintaining intestinal homeostasis and intestinal repair when recovering from infection or intestinal diseases like Crohns and ulcerative colitis. We developed a novel in vitro wound healing assay to determine the bioactive effects of various milk proteins using human small intestine cells grown on extracellular matrix. Silicone inserts are placed in a 96-well plate and enterocytes seeded around it, creating a monolayer with a cell free area. In current ongoing experiments, various pure milk proteins and isolates are added and migration quantified. This is done by performing a nuclei count and by measuring the migrated distance. The high reproducibility and gentle nature of the inserts makes this approach a good alternative to the traditional scratch assay. In perspective, it will of course be interesting to see if there are new applications for isolated bioactive milk proteins or industrial derived milk protein ingredients.

KW - Cellemigration

KW - Sårheling

KW - Bioaktivitet

M3 - Poster

Y2 - 8 October 2012 through 10 October 2012

ER -