Estimating the wound healing ability of bioactive milk proteins using an optimized cell based assay.

Publikation: KonferencebidragPosterForskning

Milk contains many different proteins of which the larger constituents like the caseins and major whey constituents are well characterized. We have for some time been studying the structure and function of proteins associated with the milk fat globule membrane like lactadherin, MUC1/15, xanthine
oxidoreductase along with minor whey constituents like osteopontin, EPV20 etc. The enterocyte migration rate is a key parameter in maintaining intestinal homeostasis and intestinal repair when recovering from infection or intestinal diseases like Crohns and ulcerative colitis. We developed a novel in vitro wound
healing assay to determine the bioactive effects of various milk proteins using human small intestine cells grown on extracellular matrix. Silicone inserts are placed in a 96-well plate and enterocytes seeded around it, creating a monolayer with a cell free area. In current ongoing experiments, various pure milk proteins and isolates are added and migration quantified. This is done by performing a nuclei count and by measuring the migrated distance. The high reproducibility and gentle nature of the inserts makes this approach a good alternative to the traditional scratch assay. In perspective, it will of course be interesting to see if there are new applications for isolated bioactive milk proteins or industrial derived milk protein ingredients.
Udgivelsesår8 okt. 2012
Antal sider1
StatusUdgivet - 8 okt. 2012
Begivenhed9th International Symposium on Milk Genomics & Human Health - Wageningen, Holland
Varighed: 8 okt. 201210 okt. 2012
Konferencens nummer: 9


Konference9th International Symposium on Milk Genomics & Human Health


  • Cellemigration, Sårheling, Bioaktivitet

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