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Estimating protein-protein interaction affinity in single living cells using Förster resonance energy transfer measurements

Publikation: Working paperForskning

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Using Förster resonance energy transfer (FRET) images we study the possibility of estimating the equilibrium dissociation constant Kd and the intrinsic FRET efficiency Em from single cells. We model the measurement uncertainty in the acquired images and use the method of total least squares for estimation based on the pixel values in the images. Considering a population of cells we demonstrate a variability in the estimates of Kd and Em that cannot be explained by measurement noise and therefore points to systematic variation between images.
OriginalsprogEngelsk
UdgiverCentre for Stochastic Geometry and advanced Bioimaging, Aarhus University
Antal sider13
StatusUdgivet - 2012
SerietitelCSGB Research Reports
Nummer12

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