EST-derived SSR markers used as anchor loci for the construction of a consensus linkage map in ryegrass (Lolium spp.)

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  • Bruno Studer, Danmark
  • Roland Kölliker, Agroscope Reckenholz-Tänikon, Research Station ART, Schweiz
  • Hilde Muylle, Institute for Agricultural and Fisheries Research (ILVO), Belgien
  • Torben Asp
  • Ursula Frei, Iowa State University, USA
  • Isabel Roldán-Ruiz, Institute for Agricultural and Fisheries Research (ILVO), Belgien
  • Philippe Barre, Institut National de Recherche Agronomique (INRA), Frankrig
  • Céline Tomaszewski, Crops Research Centre Oak Park, TEAGASC, Irland
  • Helena Meally, Crops Research Centre Oak Park, TEAGASC, Irland
  • Susanne Barth, Crops Research Centre Oak Park, TEAGASC, Irland
  • Leif Skøt, Institute of Biological, Environmental and Rural Sciences (IBERS), Storbritannien
  • Ian P Armstead, Institute of Biological, Environmental and Rural Sciences (IBERS), Storbritannien
  • Oene Dolstra, Wageningen UR Plant Breeding, Wageningen University and Research Centre (PRI), Holland
  • Thomas Lübberstedt, Iowa State University, USA
  • Molekylær Genetik og Bioteknologi
  • Institut for Genetik og Bioteknologi


Genetic markers and linkage mapping are basic prerequisites for marker-assisted selection and map-based cloning. In the case of the key grassland species Lolium spp., numerous mapping populations have been developed and characterised for various traits. Although some genetic linkage maps of these populations have been aligned with each other using publicly available DNA markers, the number of common markers among genetic maps is still low, limiting the ability to compare candidate gene and QTL locations across germplasm.


A set of 204 expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers has been assigned to map positions using eight different ryegrass mapping populations. Marker properties of a subset of 64 EST-SSRs were assessed in six to eight individuals of each mapping population and revealed 83% of the markers to be polymorphic in at least one population and an average number of alleles of 4.88. EST-SSR markers polymorphic in multiple populations served as anchor markers and allowed the construction of the first comprehensive consensus map for ryegrass. The integrated map was complemented with 97 SSRs from previously published linkage maps and finally contained 284 EST-derived and genomic SSR markers. The total map length was 742 centiMorgan (cM), ranging for individual chromosomes from 70 cM of linkage group (LG) 6 to 171 cM of LG 2.


The consensus linkage map for ryegrass based on eight mapping populations and constructed using a large set of publicly available Lolium EST-SSRs mapped for the first time together with previously mapped SSR markers will allow for consolidating existing mapping and QTL information in ryegrass. Map and markers presented here will prove to be an asset in the development for both molecular breeding of ryegrass as well as comparative genetics and genomics within grass species.

TidsskriftB M C Plant Biology
Antal sider10
StatusUdgivet - 16 aug. 2010

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