Abstract
Plasma calprotectin is a promising new biomarker of inflammatory activity and has been found to
correlate well with clinical and endoscopic activity in children and adolescents with inflammatory bowel
disease. A pediatric reference interval for plasma calprotectin has not been established for the Phadia
250 EliA™ Calprotectin fluoroenzyme immunoassay. In studies regarding pre-analytical properties,
excellent precision and stability was found. However, sensitivity to hemolysis was demonstrated. We
identified pediatric blood samples from apparently healthy children who were referred by their general
practitioner for blood sampling including measurement of hemoglobin (Hb) and C-reactive protein
(CRP). We excluded samples from children who had undergone additional blood sampling within
2 months before or after the index sample, if Hb was outside of local reference ranges or CRP levels
were above the lower limit of the measuring interval (LLM), and any samples with a hemolysis above
0.02 mmol/L. Using this algorithm, we identified 141 blood samples. No outliers were identified. We
established the following reference intervals according to CLSI C28-A3 using non-parametric statistics:
1–17 years: 16–246 μg/L. Our results may prove useful for further utilization of plasma calprotectin as a
marker of inflammation in children and adolescents with inflammatory disorders.
correlate well with clinical and endoscopic activity in children and adolescents with inflammatory bowel
disease. A pediatric reference interval for plasma calprotectin has not been established for the Phadia
250 EliA™ Calprotectin fluoroenzyme immunoassay. In studies regarding pre-analytical properties,
excellent precision and stability was found. However, sensitivity to hemolysis was demonstrated. We
identified pediatric blood samples from apparently healthy children who were referred by their general
practitioner for blood sampling including measurement of hemoglobin (Hb) and C-reactive protein
(CRP). We excluded samples from children who had undergone additional blood sampling within
2 months before or after the index sample, if Hb was outside of local reference ranges or CRP levels
were above the lower limit of the measuring interval (LLM), and any samples with a hemolysis above
0.02 mmol/L. Using this algorithm, we identified 141 blood samples. No outliers were identified. We
established the following reference intervals according to CLSI C28-A3 using non-parametric statistics:
1–17 years: 16–246 μg/L. Our results may prove useful for further utilization of plasma calprotectin as a
marker of inflammation in children and adolescents with inflammatory disorders.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Scandinavian Journal of Clinical & Laboratory Investigation |
| Vol/bind | 84 |
| Nummer | 2 |
| Sider (fra-til) | 121-124 |
| Antal sider | 4 |
| ISSN | 0036-5513 |
| DOI | |
| Status | Udgivet - apr. 2024 |