Enzyme Mimic Facilitated Artificial Cell to Mammalian Cell Signal Transfer

TY - JOUR

T1 - Enzyme Mimic Facilitated Artificial Cell to Mammalian Cell Signal Transfer

AU - Qian, Xiaomin

AU - Westensee, Isabella Nymann

AU - Fernandes, Catarina Cavalheiro

AU - Städler, Brigitte

N1 - Publisher Copyright: © 2021 Wiley-VCH GmbH

PY - 2021/8

Y1 - 2021/8

N2 - Catalyzing biochemical reactions with enzymes and communicating with neighboring cells via chemical signaling are two fundamental cellular features that play a critical role in maintaining the homeostasis of organisms. Herein, we present an artificial enzyme (AE) facilitated signal transfer between artificial cells (ACs) and mammalian HepG2 cells. We synthesize metalloporphyrins (MPs) based AEs that mimic cytochrome P450 enzymes (CYPs) to catalyze a dealkylation and a hydroxylation reaction, exemplified by the conversion of resorufin ethyl ether (REE) to resorufin and coumarin (COU) to 7-hydroxycoumarin (7-HC), respectively. The AEs are immobilized in hydrogels to produce ACs that generate the two diffusive fluorophores, which can diffuse into HepG2 cells and result in dual intracellular emissions. This work highlights the use of AEs to promote AC to mammalian signal transfer, which opens up new opportunities for integrating the synthetic and living world with a bottom-up strategy.

AB - Catalyzing biochemical reactions with enzymes and communicating with neighboring cells via chemical signaling are two fundamental cellular features that play a critical role in maintaining the homeostasis of organisms. Herein, we present an artificial enzyme (AE) facilitated signal transfer between artificial cells (ACs) and mammalian HepG2 cells. We synthesize metalloporphyrins (MPs) based AEs that mimic cytochrome P450 enzymes (CYPs) to catalyze a dealkylation and a hydroxylation reaction, exemplified by the conversion of resorufin ethyl ether (REE) to resorufin and coumarin (COU) to 7-hydroxycoumarin (7-HC), respectively. The AEs are immobilized in hydrogels to produce ACs that generate the two diffusive fluorophores, which can diffuse into HepG2 cells and result in dual intracellular emissions. This work highlights the use of AEs to promote AC to mammalian signal transfer, which opens up new opportunities for integrating the synthetic and living world with a bottom-up strategy.

KW - artificial cell

KW - artificial enzyme

KW - cytochrome P450 mimic

KW - metalloporphyrins

KW - signal transfer

UR - http://www.scopus.com/inward/record.url?scp=85110323598&partnerID=8YFLogxK

U2 - 10.1002/anie.202104904

DO - 10.1002/anie.202104904

M3 - Journal article

C2 - 34096152

AN - SCOPUS:85110323598

SN - 1433-7851

VL - 60

SP - 18704

EP - 18711

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 34

ER -