Enzyme-free colorimetric detection systems based on the DNA strand displacement competition reaction

Zhao Zhang; Victoria Birkedal; Kurt Vesterager Gothelf

doi:10.1088/1367-2630/18/5/055002

Enzyme-free colorimetric detection systems based on the DNA strand displacement competition reaction

Zhao Zhang, Victoria Birkedal, Kurt Vesterager Gothelf

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

2 Citationer (Scopus)

Abstract

The strand displacement competition assay is based on the dynamic equilibrium of the competitive hybridization of two oligonucleotides (A and B) to a third oligonucleotide (S). In the presence of an analyte that binds to a specific affinity-moiety conjugated to strand B, the equilibrium shifts, which can be detected by a shift in the fluorescence resonance energy transfer signal between dyes attached to the DNA strands. In the present study we have integrated an ATP aptamer in the strand B and demonstrated the optical detection of ATP. Furthermore we explore a new readout method using a split G-quadruplex DNAzyme for colorimetric readout of the detection of streptavidin by the naked eye. Finally, we integrate the whole G-quadruplex DNAzyme system in a single DNA strand and show that it is applicable to colorimetric detection.

Originalsprog	Engelsk
Artikelnummer	055002
Tidsskrift	New Journal of Physics
Vol/bind	18
Nummer	5
ISSN	1367-2630
DOI	https://doi.org/10.1088/1367-2630/18/5/055002
Status	Udgivet - 6 maj 2016

Adgang til dokumentet

10.1088/1367-2630/18/5/055002Licens: CC BY

Citationsformater

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title = "Enzyme-free colorimetric detection systems based on the DNA strand displacement competition reaction",

abstract = "The strand displacement competition assay is based on the dynamic equilibrium of the competitive hybridization of two oligonucleotides (A and B) to a third oligonucleotide (S). In the presence of an analyte that binds to a specific affinity-moiety conjugated to strand B, the equilibrium shifts, which can be detected by a shift in the fluorescence resonance energy transfer signal between dyes attached to the DNA strands. In the present study we have integrated an ATP aptamer in the strand B and demonstrated the optical detection of ATP. Furthermore we explore a new readout method using a split G-quadruplex DNAzyme for colorimetric readout of the detection of streptavidin by the naked eye. Finally, we integrate the whole G-quadruplex DNAzyme system in a single DNA strand and show that it is applicable to colorimetric detection.",

keywords = "Aptamer, DNA, G-quadruplex, Sensor, Strand displacement",

author = "Zhao Zhang and Victoria Birkedal and Gothelf, {Kurt Vesterager}",

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T1 - Enzyme-free colorimetric detection systems based on the DNA strand displacement competition reaction

AU - Zhang, Zhao

AU - Birkedal, Victoria

AU - Gothelf, Kurt Vesterager

PY - 2016/5/6

Y1 - 2016/5/6

N2 - The strand displacement competition assay is based on the dynamic equilibrium of the competitive hybridization of two oligonucleotides (A and B) to a third oligonucleotide (S). In the presence of an analyte that binds to a specific affinity-moiety conjugated to strand B, the equilibrium shifts, which can be detected by a shift in the fluorescence resonance energy transfer signal between dyes attached to the DNA strands. In the present study we have integrated an ATP aptamer in the strand B and demonstrated the optical detection of ATP. Furthermore we explore a new readout method using a split G-quadruplex DNAzyme for colorimetric readout of the detection of streptavidin by the naked eye. Finally, we integrate the whole G-quadruplex DNAzyme system in a single DNA strand and show that it is applicable to colorimetric detection.

AB - The strand displacement competition assay is based on the dynamic equilibrium of the competitive hybridization of two oligonucleotides (A and B) to a third oligonucleotide (S). In the presence of an analyte that binds to a specific affinity-moiety conjugated to strand B, the equilibrium shifts, which can be detected by a shift in the fluorescence resonance energy transfer signal between dyes attached to the DNA strands. In the present study we have integrated an ATP aptamer in the strand B and demonstrated the optical detection of ATP. Furthermore we explore a new readout method using a split G-quadruplex DNAzyme for colorimetric readout of the detection of streptavidin by the naked eye. Finally, we integrate the whole G-quadruplex DNAzyme system in a single DNA strand and show that it is applicable to colorimetric detection.

KW - Aptamer

KW - DNA

KW - G-quadruplex

KW - Sensor

KW - Strand displacement

U2 - 10.1088/1367-2630/18/5/055002

DO - 10.1088/1367-2630/18/5/055002

M3 - Journal article

SN - 1367-2630

VL - 18

JO - New Journal of Physics

JF - New Journal of Physics

IS - 5

M1 - 055002

ER -