Environmental DNA from Seawater Samples Correlate with Trawl Catches of Subarctic, Deepwater Fishes

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  • Philip Francis Thomsen
  • Peter Rask Moller, Univ Copenhagen, University of Copenhagen, Nat Hist Museum Denmark, Sect EvoGen
  • ,
  • Eva Egelyng Sigsgaard
  • Steen Wilhelm Knudsen
  • Ole Ankjaer Jorgensen, Greenland Inst Nat Resources, Greenland Institute of Natural Resources
  • ,
  • Eske Willerslev, Sanger Inst, Wellcome Trust Sanger Institute

Remote polar and deepwater fish faunas are under pressure from ongoing climate change and increasing fishing effort. However, these fish communities are difficult to monitor for logistic and financial reasons. Currently, monitoring of marine fishes largely relies on invasive techniques such as bottom trawling, and on official reporting of global catches, which can be unreliable. Thus, there is need for alternative and non-invasive techniques for qualitative and quantitative oceanic fish surveys. Here we report environmental DNA (eDNA) metabarcoding of seawater samples from continental slope depths in Southwest Greenland. We collected seawater samples at depths of 188-918 m and compared seawater eDNA to catch data from trawling. We used Illumina sequencing of PCR products to demonstrate that eDNA reads show equivalence to fishing catch data obtained from trawling. Twenty-six families were found with both trawling and eDNA, while three families were found only with eDNA and two families were found only with trawling. Key commercial fish species for Greenland were the most abundant species in both eDNA reads and biomass catch, and interpolation of eDNA abundances between sampling sites showed good correspondence with catch sizes. Environmental DNA sequence reads from the fish assemblages correlated with biomass and abundance data obtained from trawling. Interestingly, the Greenland shark (Somniosus microcephalus) showed high abundance of eDNA reads despite only a single specimen being caught, demonstrating the relevance of the eDNA approach for large species that can probably avoid bottom trawls in most cases. Quantitative detection of marine fish using eDNA remains to be tested further to ascertain whether this technique is able to yield credible results for routine application in fisheries. Nevertheless, our study demonstrates that eDNA reads can be used as a qualitative and quantitative proxy for marine fish assemblages in deepwater oceanic habitats. This relates directly to applied fisheries as well as to monitoring effects of ongoing climate change on marine biodiversity-especially in polar ecosystems.

OriginalsprogEngelsk
Artikelnummer0165252
TidsskriftPLOS ONE
Vol/bind11
Nummer11
Antal sider22
ISSN1932-6203
DOI
StatusUdgivet - 16 nov. 2016
Eksternt udgivetJa

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