Entire mitral valve reconstruction using porcine extracellular matrix: static in vitro evaluation

Marcell J Tjørnild; Lisa Carlson Hanse; Søren N Skov; Sten L Nielsen; J Michael Hasenkam; Diana M Røpcke

doi:10.1093/ejcts/ezy416

Entire mitral valve reconstruction using porcine extracellular matrix: static in vitro evaluation

Marcell J Tjørnild, Lisa Carlson Hanse, Søren N Skov, Sten L Nielsen, J Michael Hasenkam, Diana M Røpcke

Institut for Klinisk Medicin - Hjerte-, Lunge- og Karkirurgi

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

9 Citationer (Scopus)

Abstract

To investigate the feasibility of reconstruction of the entire mitral valve using a tube graft made of 2-ply small intestinal submucosa extracellular matrix in vitro. Seven explanted mitral valves with intact subvalvular apparatus from 80 kg pigs were evaluated in a left heart simulator and served as controls. After testing the native valve, the leaflets and chordae tendineae were explanted, and the 2-ply small intestinal submucosa extracellular matrix (CorMatrixVR ; Cardiovascular Inc., Alpharetta, GA, USA) tube graft was implanted. The characterization was based on geometric data from digital images, papillary muscle force, annular tethering force and leaflet pressure force. The tube grafts were fully functional without any signs of leakage, tearing or rupture during incrementally increased pressures from 0mmHg to 120 mmHg. The posterior leaflet moved anteriorly and became larger after reconstruction when compared with the native valve. However, the mid coaptation point was preserved. The anterior papillary muscle force decreased significantly (5.2N vs 4.4 N, P = 0.022 at 120 mmHg), and the posterior papillary muscle force increased significantly (4.8N vs 5.6 N, P = 0.017 at 120 mmHg) after reconstruction. The entire mitral valvular and subvalvular reconstruction with a 2-ply small intestinal submucosa extracellular matrix tube graft is feasible in an in vitro model. Our method of reconstruction increased the convexity of the anterior leaflet s coaptation line and significantly redistributed the papillary muscle force towards the posterior papillary muscle. These promising results and the prospect of the entire mitral valvular and subvalvular reconstruction warrant further in vivo evaluations..

Originalsprog	Engelsk
Tidsskrift	European Journal of Cardio-Thoracic Surgery
Vol/bind	55
Nummer	6
Sider (fra-til)	1095-1103
Antal sider	9
ISSN	1010-7940
DOI	https://doi.org/10.1093/ejcts/ezy416
Status	Udgivet - jun. 2019

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10.1093/ejcts/ezy416

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title = "Entire mitral valve reconstruction using porcine extracellular matrix: static in vitro evaluation",

abstract = "To investigate the feasibility of reconstruction of the entire mitral valve using a tube graft made of 2-ply small intestinal submucosa extracellular matrix in vitro. Seven explanted mitral valves with intact subvalvular apparatus from 80 kg pigs were evaluated in a left heart simulator and served as controls. After testing the native valve, the leaflets and chordae tendineae were explanted, and the 2-ply small intestinal submucosa extracellular matrix (CorMatrixVR ; Cardiovascular Inc., Alpharetta, GA, USA) tube graft was implanted. The characterization was based on geometric data from digital images, papillary muscle force, annular tethering force and leaflet pressure force. The tube grafts were fully functional without any signs of leakage, tearing or rupture during incrementally increased pressures from 0mmHg to 120 mmHg. The posterior leaflet moved anteriorly and became larger after reconstruction when compared with the native valve. However, the mid coaptation point was preserved. The anterior papillary muscle force decreased significantly (5.2N vs 4.4 N, P = 0.022 at 120 mmHg), and the posterior papillary muscle force increased significantly (4.8N vs 5.6 N, P = 0.017 at 120 mmHg) after reconstruction. The entire mitral valvular and subvalvular reconstruction with a 2-ply small intestinal submucosa extracellular matrix tube graft is feasible in an in vitro model. Our method of reconstruction increased the convexity of the anterior leaflet s coaptation line and significantly redistributed the papillary muscle force towards the posterior papillary muscle. These promising results and the prospect of the entire mitral valvular and subvalvular reconstruction warrant further in vivo evaluations..",

keywords = "2-Ply small intestinal submucosa extracellular matrix, Cormatrix, Leaflet pressure force, Mitral valve, Mitral valve reconstruction, Papillary muscle force",

author = "Tj{\o}rnild, {Marcell J} and {Carlson Hanse}, Lisa and Skov, {S{\o}ren N} and Nielsen, {Sten L} and Hasenkam, {J Michael} and R{\o}pcke, {Diana M}",

year = "2019",

month = jun,

doi = "10.1093/ejcts/ezy416",

language = "English",

volume = "55",

pages = "1095--1103",

journal = "European Journal of Cardio-Thoracic Surgery",

issn = "1010-7940",

publisher = "European Association for Cardio-Thoracic Surgery",

number = "6",

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Entire mitral valve reconstruction using porcine extracellular matrix: static in vitro evaluation. / Tjørnild, Marcell J ; Carlson Hanse, Lisa ; Skov, Søren N et al.
I: European Journal of Cardio-Thoracic Surgery, Bind 55, Nr. 6, 06.2019, s. 1095-1103.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - Entire mitral valve reconstruction using porcine extracellular matrix

T2 - static in vitro evaluation

AU - Tjørnild, Marcell J

AU - Carlson Hanse, Lisa

AU - Skov, Søren N

AU - Nielsen, Sten L

AU - Hasenkam, J Michael

AU - Røpcke, Diana M

PY - 2019/6

Y1 - 2019/6

N2 - To investigate the feasibility of reconstruction of the entire mitral valve using a tube graft made of 2-ply small intestinal submucosa extracellular matrix in vitro. Seven explanted mitral valves with intact subvalvular apparatus from 80 kg pigs were evaluated in a left heart simulator and served as controls. After testing the native valve, the leaflets and chordae tendineae were explanted, and the 2-ply small intestinal submucosa extracellular matrix (CorMatrixVR ; Cardiovascular Inc., Alpharetta, GA, USA) tube graft was implanted. The characterization was based on geometric data from digital images, papillary muscle force, annular tethering force and leaflet pressure force. The tube grafts were fully functional without any signs of leakage, tearing or rupture during incrementally increased pressures from 0mmHg to 120 mmHg. The posterior leaflet moved anteriorly and became larger after reconstruction when compared with the native valve. However, the mid coaptation point was preserved. The anterior papillary muscle force decreased significantly (5.2N vs 4.4 N, P = 0.022 at 120 mmHg), and the posterior papillary muscle force increased significantly (4.8N vs 5.6 N, P = 0.017 at 120 mmHg) after reconstruction. The entire mitral valvular and subvalvular reconstruction with a 2-ply small intestinal submucosa extracellular matrix tube graft is feasible in an in vitro model. Our method of reconstruction increased the convexity of the anterior leaflet s coaptation line and significantly redistributed the papillary muscle force towards the posterior papillary muscle. These promising results and the prospect of the entire mitral valvular and subvalvular reconstruction warrant further in vivo evaluations..

AB - To investigate the feasibility of reconstruction of the entire mitral valve using a tube graft made of 2-ply small intestinal submucosa extracellular matrix in vitro. Seven explanted mitral valves with intact subvalvular apparatus from 80 kg pigs were evaluated in a left heart simulator and served as controls. After testing the native valve, the leaflets and chordae tendineae were explanted, and the 2-ply small intestinal submucosa extracellular matrix (CorMatrixVR ; Cardiovascular Inc., Alpharetta, GA, USA) tube graft was implanted. The characterization was based on geometric data from digital images, papillary muscle force, annular tethering force and leaflet pressure force. The tube grafts were fully functional without any signs of leakage, tearing or rupture during incrementally increased pressures from 0mmHg to 120 mmHg. The posterior leaflet moved anteriorly and became larger after reconstruction when compared with the native valve. However, the mid coaptation point was preserved. The anterior papillary muscle force decreased significantly (5.2N vs 4.4 N, P = 0.022 at 120 mmHg), and the posterior papillary muscle force increased significantly (4.8N vs 5.6 N, P = 0.017 at 120 mmHg) after reconstruction. The entire mitral valvular and subvalvular reconstruction with a 2-ply small intestinal submucosa extracellular matrix tube graft is feasible in an in vitro model. Our method of reconstruction increased the convexity of the anterior leaflet s coaptation line and significantly redistributed the papillary muscle force towards the posterior papillary muscle. These promising results and the prospect of the entire mitral valvular and subvalvular reconstruction warrant further in vivo evaluations..

KW - 2-Ply small intestinal submucosa extracellular matrix

KW - Cormatrix

KW - Leaflet pressure force

KW - Mitral valve

KW - Mitral valve reconstruction

KW - Papillary muscle force

U2 - 10.1093/ejcts/ezy416

DO - 10.1093/ejcts/ezy416

M3 - Journal article

C2 - 30597010

SN - 1010-7940

VL - 55

SP - 1095

EP - 1103

JO - European Journal of Cardio-Thoracic Surgery

JF - European Journal of Cardio-Thoracic Surgery

IS - 6

ER -

Entire mitral valve reconstruction using porcine extracellular matrix: static in vitro evaluation

Abstract

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