Engineered nanostructured β-sheet peptides protect membrane proteins

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  • Houchao Tao, Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA., Danmark
  • Sung Chang Lee, Danmark
  • Arne Möller
  • Rituparna Sinha Roy, Danmark
  • Fai Yiu Siu, Danmark
  • Jörg Zimmermann, Danmark
  • Raymond C Stevens, Danmark
  • Clinton S Potter, Danmark
  • Bridget Carragher
  • ,
  • Qinghai Zhang, Danmark

We designed β-strand peptides that stabilize integral membrane proteins (IMPs). β-strand peptides self-assemble in solution as filaments and become restructured upon association with IMPs; resulting IMP-β-strand peptide complexes resisted aggregation when diluted in detergent-free buffer and were visible as stable, single particles with low detergent background in electron micrographs. β-strand peptides enabled clear visualization of flexible conformations in the highly dynamic ATP-binding cassette (ABC) transporter MsbA.

OriginalsprogEngelsk
TidsskriftNature Methods
Vol/bind10
Nummer8
Sider (fra-til)759-61
Antal sider3
ISSN1548-7091
DOI
StatusUdgivet - aug. 2013
Eksternt udgivetJa

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