Electrospun PVA-PCL-HAB scaffold for craniofacial bone regeneration

TY - ABST

T1 - Electrospun PVA-PCL-HAB scaffold for craniofacial bone regeneration

AU - Prabha, Rahul

AU - Kraft, David Christian Evar

AU - Melsen, Birte

AU - Varma, H.

AU - Nair, P. D.

AU - Kjems, Jørgen

AU - Kassem, Moustapha

PY - 2015

Y1 - 2015

N2 - Bone tissue engineering for craniofacial region is considered challenging owing to its physiologic and anatomical complexities. A porous bioactive scaffold promoting osteogenesis and angio- genesis is required for clinical applications. We have developed an electrospun polyvinyl alcohol (PVA) poly-caprolactone (PCL)- triphasic bioceramic(HAB) scaffold to biomimic native tissue and we tested its ability to support osteogenic differentiation of stromal stem cells ( MSC) and its suitability for regeneration of craniofa- cial defects. Physiochemical characterizations of the scaffold, including con- tact angle measurements, showed that PVA-PCL-HAB was more hydrophilic than PCL alone or PCL-HAB combined (P < 0.05). Ion release profile studies using ICP-OES of the scaffold showed release of calcium and silica ions, required for initiation of bioactivity. SEM and EDS analyses revealed apatite formation on stimulated body fluid immersed scaffold samples. Culturing human adult dental pulp stem cells (DPSC) and human bone marrow derived MSC seeded on PVA-PCL-HAB scaffold showed enhanced cell proliferation and in vitro osteoblastic differentiation. Cell-containing scaffolds were implanted subcutaneously in immune deficient mice. Histologic ex- amination of retrieved implant sections stained with H&E, Col- lagenType I and Human Vimentin antibody demonstrated that the cells survived in vivo in the implants for at least 8 weeks with evidence of osteoblastic differentiation and angiogenesis within the implants. Our results suggest that PVA-PCL-HAB scaffold can support growth and osteoblast differentiation of MSC and thus should be considered for clinical use in craniofacial tissue regeneration

AB - Bone tissue engineering for craniofacial region is considered challenging owing to its physiologic and anatomical complexities. A porous bioactive scaffold promoting osteogenesis and angio- genesis is required for clinical applications. We have developed an electrospun polyvinyl alcohol (PVA) poly-caprolactone (PCL)- triphasic bioceramic(HAB) scaffold to biomimic native tissue and we tested its ability to support osteogenic differentiation of stromal stem cells ( MSC) and its suitability for regeneration of craniofa- cial defects. Physiochemical characterizations of the scaffold, including con- tact angle measurements, showed that PVA-PCL-HAB was more hydrophilic than PCL alone or PCL-HAB combined (P < 0.05). Ion release profile studies using ICP-OES of the scaffold showed release of calcium and silica ions, required for initiation of bioactivity. SEM and EDS analyses revealed apatite formation on stimulated body fluid immersed scaffold samples. Culturing human adult dental pulp stem cells (DPSC) and human bone marrow derived MSC seeded on PVA-PCL-HAB scaffold showed enhanced cell proliferation and in vitro osteoblastic differentiation. Cell-containing scaffolds were implanted subcutaneously in immune deficient mice. Histologic ex- amination of retrieved implant sections stained with H&E, Col- lagenType I and Human Vimentin antibody demonstrated that the cells survived in vivo in the implants for at least 8 weeks with evidence of osteoblastic differentiation and angiogenesis within the implants. Our results suggest that PVA-PCL-HAB scaffold can support growth and osteoblast differentiation of MSC and thus should be considered for clinical use in craniofacial tissue regeneration

U2 - 10.1089/ten.tea.2015.5000.abstracts

DO - 10.1089/ten.tea.2015.5000.abstracts

M3 - Conference abstract in journal

C2 - 26317531

SN - 1937-3341

VL - 21

SP - S-58

JO - Tissue Engineering. Part A

JF - Tissue Engineering. Part A

IS - Supplement 1

T2 - 2015 4th TERMIS World Congress

Y2 - 8 September 2015 through 11 September 2015

ER -