Electromagnetic Stimulated Anisotropic Hydrogels for Guided Neuronal Morphogenesis

Bjarke Nørrehvedde Jensen; Tong Tong; Grith Skovborg; Yuge Zhang; Christoph Alexander Müller; Cecilie Linneberg Matthiesen; Jens Toft Eschen; Kirstine Juul Elbæk; Yuting Wang; Lone Tjener Pallesen; Dror Seliktar; Mingdong Dong; Christian Bjerggaard Vægter; Anders Rosendal Korshoej; Menglin Chen

doi:10.1101/2024.09.29.615659

Electromagnetic Stimulated Anisotropic Hydrogels for Guided Neuronal Morphogenesis

Bjarke Nørrehvedde Jensen, Tong Tong, Grith Skovborg, Yuge Zhang, Christoph Alexander Müller, Cecilie Linneberg Matthiesen, Jens Toft Eschen, Kirstine Juul Elbæk, Yuting Wang, Lone Tjener Pallesen, Dror Seliktar, Mingdong Dong, Christian Bjerggaard Vægter, Anders Rosendal Korshoej, Menglin Chen

Publikation: Working paper/Preprint › Preprint

Abstract

While electrical circuits are a fundamental part of neural activity, the soft microstructural extracellular environment plays a vital role in driving the morphogenesis of neurons. To leverage this, we combine a wireless electromagnetic neural stimulation system with anisotropic GelMA hydrogels to promote and guide neural morphogensis. We show that oriented GelMA hydrogels support anisotropic growth of dorsal root ganglion as potential peripheral neural guide conduit and promote the formation of hippocampal neurospheres networking along the fiber direction. The central nervous system has limited regeneration due to glial scar formation, the presence of the perineuronal network, and limited upregulation of growth-associated genes post traumatic injuries. The system was further tested on human cortical brain tissue where hydrogel microfilaments promoted neurite outgrowth in a white-matter-mimicking manner between two pieces of brain slices, opening the possibility of central neural tissue regeneration.

Originalsprog	Engelsk
Udgiver	bioRxiv
DOI	https://doi.org/10.1101/2024.09.29.615659
Status	Udgivet - 4 okt. 2024

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10.1101/2024.09.29.615659Licens: Ikke-specificeret

Citationsformater

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abstract = "While electrical circuits are a fundamental part of neural activity, the soft microstructural extracellular environment plays a vital role in driving the morphogenesis of neurons. To leverage this, we combine a wireless electromagnetic neural stimulation system with anisotropic GelMA hydrogels to promote and guide neural morphogensis. We show that oriented GelMA hydrogels support anisotropic growth of dorsal root ganglion as potential peripheral neural guide conduit and promote the formation of hippocampal neurospheres networking along the fiber direction. The central nervous system has limited regeneration due to glial scar formation, the presence of the perineuronal network, and limited upregulation of growth-associated genes post traumatic injuries. The system was further tested on human cortical brain tissue where hydrogel microfilaments promoted neurite outgrowth in a white-matter-mimicking manner between two pieces of brain slices, opening the possibility of central neural tissue regeneration.",

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AU - Jensen, Bjarke Nørrehvedde

AU - Tong, Tong

AU - Skovborg, Grith

AU - Zhang, Yuge

AU - Müller, Christoph Alexander

AU - Matthiesen, Cecilie Linneberg

AU - Eschen, Jens Toft

AU - Elbæk, Kirstine Juul

AU - Wang, Yuting

AU - Pallesen, Lone Tjener

AU - Seliktar, Dror

AU - Dong, Mingdong

AU - Vægter, Christian Bjerggaard

AU - Korshoej, Anders Rosendal

AU - Chen, Menglin

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N2 - While electrical circuits are a fundamental part of neural activity, the soft microstructural extracellular environment plays a vital role in driving the morphogenesis of neurons. To leverage this, we combine a wireless electromagnetic neural stimulation system with anisotropic GelMA hydrogels to promote and guide neural morphogensis. We show that oriented GelMA hydrogels support anisotropic growth of dorsal root ganglion as potential peripheral neural guide conduit and promote the formation of hippocampal neurospheres networking along the fiber direction. The central nervous system has limited regeneration due to glial scar formation, the presence of the perineuronal network, and limited upregulation of growth-associated genes post traumatic injuries. The system was further tested on human cortical brain tissue where hydrogel microfilaments promoted neurite outgrowth in a white-matter-mimicking manner between two pieces of brain slices, opening the possibility of central neural tissue regeneration.

AB - While electrical circuits are a fundamental part of neural activity, the soft microstructural extracellular environment plays a vital role in driving the morphogenesis of neurons. To leverage this, we combine a wireless electromagnetic neural stimulation system with anisotropic GelMA hydrogels to promote and guide neural morphogensis. We show that oriented GelMA hydrogels support anisotropic growth of dorsal root ganglion as potential peripheral neural guide conduit and promote the formation of hippocampal neurospheres networking along the fiber direction. The central nervous system has limited regeneration due to glial scar formation, the presence of the perineuronal network, and limited upregulation of growth-associated genes post traumatic injuries. The system was further tested on human cortical brain tissue where hydrogel microfilaments promoted neurite outgrowth in a white-matter-mimicking manner between two pieces of brain slices, opening the possibility of central neural tissue regeneration.

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BT - Electromagnetic Stimulated Anisotropic Hydrogels for Guided Neuronal Morphogenesis

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