Electrochemical Label-Free Aptasensor for Specific Analysis of Dopamine in Serum in the Presence of Structurally Related Neurotransmitters

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Electrochemical Label-Free Aptasensor for Specific Analysis of Dopamine in Serum in the Presence of Structurally Related Neurotransmitters. / Álvarez-Martos, Isabel ; Ferapontova, Elena.

I: Analytical Chemistry, Bind 88, Nr. 7, 2016, s. 3608-3616.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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@article{50ac88d966e34fe49dd4acfde73f04a9,
title = "Electrochemical Label-Free Aptasensor for Specific Analysis of Dopamine in Serum in the Presence of Structurally Related Neurotransmitters",
abstract = "Cellular and brain metabolism of dopamine can becorrelated with a number of neurodegenerative disorders, and as such,in vivo analysis of dopamine in the presence of structurally relatedneurotransmitters (NT) represents a holy grail of neuroscience.Interference from those NTs generally does not allow selectiveelectroanalysis of dopamine, which redox transformation overlaps withthose of other catecholamines. In our previous work, we reported anelectrochemical RNA-aptamer-based biosensor for specific analysis ofdopamine (Analytical Chemistry, 2013; Vol. 85, p 121). However, theoverall design of the biosensor restricted its stability and impeded itsoperation in serum. Here, we show that specific biorecognition andelectroanalysis of dopamine in serum can be performed by the RNAaptamer tethered to cysteamine-modified gold electrodes via thealkanethiol linker. The stabilized dopamine aptasensor allowedcontinuous 20 h amperometric analysis of dopamine in 10% serum within the physiologically important 0.11 μM rangeand in the presence of catechol and such dopamine precursors and metabolites as norepinephrine and L-DOPA. In a flowinjectionmode, the aptasensor response to dopamine was ∼1 s, the sensitivity of analysis, optimized by adjusting the aptamersurface coverage, was 67 ± 1 nA μM−1 cm−2, and the dopamine LOD was 62 nM. The proposed design of the aptasensor,exploiting both the aptamer alkanethiol tethering to the electrode and screening of the catecholamine-aptamer electrostaticinteractions, allows direct monitoring of dopamine levels in biological fluids in the presence of competitive NT and thus may befurther applicable in biomedical research.",
keywords = "DOPAMINE, Electrochemistry, SERUM",
author = "Isabel {\'A}lvarez-Martos and Elena Ferapontova",
year = "2016",
doi = "10.1021/acs.analchem.5b04207",
language = "English",
volume = "88",
pages = "3608--3616",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "AMER CHEMICAL SOC",
number = "7",

}

RIS

TY - JOUR

T1 - Electrochemical Label-Free Aptasensor for Specific Analysis of Dopamine in Serum in the Presence of Structurally Related Neurotransmitters

AU - Álvarez-Martos, Isabel

AU - Ferapontova, Elena

PY - 2016

Y1 - 2016

N2 - Cellular and brain metabolism of dopamine can becorrelated with a number of neurodegenerative disorders, and as such,in vivo analysis of dopamine in the presence of structurally relatedneurotransmitters (NT) represents a holy grail of neuroscience.Interference from those NTs generally does not allow selectiveelectroanalysis of dopamine, which redox transformation overlaps withthose of other catecholamines. In our previous work, we reported anelectrochemical RNA-aptamer-based biosensor for specific analysis ofdopamine (Analytical Chemistry, 2013; Vol. 85, p 121). However, theoverall design of the biosensor restricted its stability and impeded itsoperation in serum. Here, we show that specific biorecognition andelectroanalysis of dopamine in serum can be performed by the RNAaptamer tethered to cysteamine-modified gold electrodes via thealkanethiol linker. The stabilized dopamine aptasensor allowedcontinuous 20 h amperometric analysis of dopamine in 10% serum within the physiologically important 0.11 μM rangeand in the presence of catechol and such dopamine precursors and metabolites as norepinephrine and L-DOPA. In a flowinjectionmode, the aptasensor response to dopamine was ∼1 s, the sensitivity of analysis, optimized by adjusting the aptamersurface coverage, was 67 ± 1 nA μM−1 cm−2, and the dopamine LOD was 62 nM. The proposed design of the aptasensor,exploiting both the aptamer alkanethiol tethering to the electrode and screening of the catecholamine-aptamer electrostaticinteractions, allows direct monitoring of dopamine levels in biological fluids in the presence of competitive NT and thus may befurther applicable in biomedical research.

AB - Cellular and brain metabolism of dopamine can becorrelated with a number of neurodegenerative disorders, and as such,in vivo analysis of dopamine in the presence of structurally relatedneurotransmitters (NT) represents a holy grail of neuroscience.Interference from those NTs generally does not allow selectiveelectroanalysis of dopamine, which redox transformation overlaps withthose of other catecholamines. In our previous work, we reported anelectrochemical RNA-aptamer-based biosensor for specific analysis ofdopamine (Analytical Chemistry, 2013; Vol. 85, p 121). However, theoverall design of the biosensor restricted its stability and impeded itsoperation in serum. Here, we show that specific biorecognition andelectroanalysis of dopamine in serum can be performed by the RNAaptamer tethered to cysteamine-modified gold electrodes via thealkanethiol linker. The stabilized dopamine aptasensor allowedcontinuous 20 h amperometric analysis of dopamine in 10% serum within the physiologically important 0.11 μM rangeand in the presence of catechol and such dopamine precursors and metabolites as norepinephrine and L-DOPA. In a flowinjectionmode, the aptasensor response to dopamine was ∼1 s, the sensitivity of analysis, optimized by adjusting the aptamersurface coverage, was 67 ± 1 nA μM−1 cm−2, and the dopamine LOD was 62 nM. The proposed design of the aptasensor,exploiting both the aptamer alkanethiol tethering to the electrode and screening of the catecholamine-aptamer electrostaticinteractions, allows direct monitoring of dopamine levels in biological fluids in the presence of competitive NT and thus may befurther applicable in biomedical research.

KW - DOPAMINE

KW - Electrochemistry

KW - SERUM

U2 - 10.1021/acs.analchem.5b04207

DO - 10.1021/acs.analchem.5b04207

M3 - Journal article

C2 - 26916821

VL - 88

SP - 3608

EP - 3616

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 7

ER -