Electrochemical ELASA: improving early cancer detection and monitoring

TY - JOUR

T1 - Electrochemical ELASA

T2 - improving early cancer detection and monitoring

AU - Díaz-Fernández, Ana

AU - Ferapontova, Elena E.

PY - 2023/7

Y1 - 2023/7

N2 - The discovery of new molecular biomarkers of cancer during the last decades and the development of new diagnostic devices exploiting those have significantly contributed to the clinical analysis of cancer and to improve the outcomes. Among those, liquid biopsy sensors exploiting aptamers for the detection of cancer biomarkers in body fluids are useful and accurate tools for a fast and inexpensive non-invasive screening of population. The incorporation of aptamers in electrochemical sandwich biosensors using enzyme labels, a so-called ELASA, has demonstrated its utility to improve the detection schemes. In this review, we overview the existing ELASA assays for numerous cancer biomarkers as alternatives to the traditional ELISA and discuss their possibilities to reach the market, currently dominated by optical immunoassays. Graphical abstract: [Figure not available: see fulltext.]

AB - The discovery of new molecular biomarkers of cancer during the last decades and the development of new diagnostic devices exploiting those have significantly contributed to the clinical analysis of cancer and to improve the outcomes. Among those, liquid biopsy sensors exploiting aptamers for the detection of cancer biomarkers in body fluids are useful and accurate tools for a fast and inexpensive non-invasive screening of population. The incorporation of aptamers in electrochemical sandwich biosensors using enzyme labels, a so-called ELASA, has demonstrated its utility to improve the detection schemes. In this review, we overview the existing ELASA assays for numerous cancer biomarkers as alternatives to the traditional ELISA and discuss their possibilities to reach the market, currently dominated by optical immunoassays. Graphical abstract: [Figure not available: see fulltext.]

KW - Aptamer electrodes

KW - Cancer diagnostics

KW - ELASA

KW - Electroanalysis

KW - Electrochemical sensors

KW - Enzymes

U2 - 10.1007/s00216-023-04546-5

DO - 10.1007/s00216-023-04546-5

M3 - Review

C2 - 36702904

AN - SCOPUS:85146843305

SN - 1618-2642

VL - 415

SP - 3831

EP - 3846

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

IS - 18

ER -