EF-Tu dynamics during pre-translocation complex formation: EF-Tu•GDP exits the ribosome via two different pathways

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DOI

  • Wei Liu, Univ Penn, University of Pennsylvania, Dept Chem
  • ,
  • Chunlai Chen, Univ Penn, University of Pennsylvania, Perelman Sch Med, Penn Muscle Inst, Danmark
  • Darius Kavaliauskas
  • ,
  • Charlotte R. Knudsen
  • Yale E. Goldman, Univ Penn, University of Pennsylvania, Perelman Sch Med, Penn Muscle Inst, Danmark
  • Barry S. Cooperman, Univ Penn, University of Pennsylvania, Dept Chem, Danmark

The G-protein EF-Tu, which undergoes a major conformational change when EF-Tu center dot GTP is converted to EF-Tu center dot GTP, forms part of an aminoacyl(aa)-tRNA center dot EF-Tu center dot GTP ternary complex (TC) that accelerates the binding of aa-tRNA to the ribosome during peptide elongation. Such binding, placing a portion of EF-Tu in contact with the GTPase Associated Center (GAC), is followed by GTP hydrolysis and Pi release, and results in formation of a pretranslocation (PRE) complex. Although tRNA movement through the ribosome during PRE complex formation has been extensively studied, comparatively little is known about the dynamics of EF-Tu interaction with either the ribosome or aa-tRNA. Here we examine these dynamics, utilizing ensemble and single molecule assays employing fluorescent labeled derivatives of EF-Tu, tRNA, and the ribosome to measure changes in either FRET efficiency or fluorescence intensity during PRE complex formation. Our results indicate that ribosome-bound EF-Tu separates from the GAC prior to its full separation from aa-tRNA, and suggest that EF-Tu center dot GDP dissociates from the ribosome by two different pathways. These pathways correspond to either reversible EF-Tu center dot GTP dissociation from the ribosome prior to the major conformational change in EF-Tu that follows GTP hydrolysis, or irreversible dissociation after or concomitant with this conformational change.

OriginalsprogEngelsk
TidsskriftNucleic Acids Research
Vol/bind43
Nummer19
Sider (fra-til)9519-9528
Antal sider10
ISSN0305-1048
DOI
StatusUdgivet - 2015

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