Efficient in vivo gene transfer to xenotransplanted human skin by lentivirus-mediated, but not by AAV-directed, gene delivery

Publikation: KonferencebidragPosterForskningpeer review

Standard

Efficient in vivo gene transfer to xenotransplanted human skin by lentivirus-mediated, but not by AAV-directed, gene delivery. / Jakobsen, Maria Vad; Askou, Anne Louise; Dokkedahl, Karin Stenderup; Kjeldsen, Cecilia Rosada; Dagnæs-Hansen, Frederik; Jensen, Thomas G.; Corydon, Thomas Juhl; Mikkelsen, Jacob Giehm; Aagaard, Lars.

2015. Poster session præsenteret ved Annual Congress The European Society of Gene and Cell Therapy (ESGCT), Helsinki, Finland.

Publikation: KonferencebidragPosterForskningpeer review

Harvard

Jakobsen, MV, Askou, AL, Dokkedahl, KS, Kjeldsen, CR, Dagnæs-Hansen, F, Jensen, TG, Corydon, TJ, Mikkelsen, JG & Aagaard, L 2015, 'Efficient in vivo gene transfer to xenotransplanted human skin by lentivirus-mediated, but not by AAV-directed, gene delivery', Annual Congress The European Society of Gene and Cell Therapy (ESGCT), Helsinki, Finland, 17/09/2015 - 20/09/2015.

APA

Jakobsen, M. V., Askou, A. L., Dokkedahl, K. S., Kjeldsen, C. R., Dagnæs-Hansen, F., Jensen, T. G., Corydon, T. J., Mikkelsen, J. G., & Aagaard, L. (2015). Efficient in vivo gene transfer to xenotransplanted human skin by lentivirus-mediated, but not by AAV-directed, gene delivery. Poster session præsenteret ved Annual Congress The European Society of Gene and Cell Therapy (ESGCT), Helsinki, Finland.

CBE

Jakobsen MV, Askou AL, Dokkedahl KS, Kjeldsen CR, Dagnæs-Hansen F, Jensen TG, Corydon TJ, Mikkelsen JG, Aagaard L. 2015. Efficient in vivo gene transfer to xenotransplanted human skin by lentivirus-mediated, but not by AAV-directed, gene delivery. Poster session præsenteret ved Annual Congress The European Society of Gene and Cell Therapy (ESGCT), Helsinki, Finland.

MLA

Jakobsen, Maria Vad o.a.. Efficient in vivo gene transfer to xenotransplanted human skin by lentivirus-mediated, but not by AAV-directed, gene delivery. Annual Congress The European Society of Gene and Cell Therapy (ESGCT), 17 sep. 2015, Helsinki, Finland, Poster, 2015. 1 s.

Vancouver

Jakobsen MV, Askou AL, Dokkedahl KS, Kjeldsen CR, Dagnæs-Hansen F, Jensen TG o.a.. Efficient in vivo gene transfer to xenotransplanted human skin by lentivirus-mediated, but not by AAV-directed, gene delivery. 2015. Poster session præsenteret ved Annual Congress The European Society of Gene and Cell Therapy (ESGCT), Helsinki, Finland.

Author

Bibtex

@conference{0fbb2634142c49409221d13a914d8035,
title = "Efficient in vivo gene transfer to xenotransplanted human skin by lentivirus-mediated, but not by AAV-directed, gene delivery",
abstract = "Skin is an easily accessible organ, and therapeutic gene transfer to skin remains an attractive alternative for treatment of skin diseases. We compared the potential usefulness of various serotypes of recombinant AAV vectors and lentiviral vectors for gene transfer to human skin in a xenotransplanted mouse model. Vector constructs encoding firefly luciferase were packaged in AAV capsids of serotype 1, 2, 5, 6, 8, and 9 and separately administered by intradermal injection in human skin transplants. For all serotypes, live bioimagingdemonstrated low levels of transgene expression in the human skin graft, and firefly luciferase expression was observed primarily in neighboring tissue beneath or surrounding the graft. In contrast, gene delivery by intradermally injected lentiviral vectors was efficient and led to extensive and persistent firefly luciferase expression within the human skin graft only. The study demonstrates limited capacity of single-stranded AAV vectors of six commonly used serotypes for gene delivery to human skin in vivo.",
author = "Jakobsen, {Maria Vad} and Askou, {Anne Louise} and Dokkedahl, {Karin Stenderup} and Kjeldsen, {Cecilia Rosada} and Frederik Dagn{\ae}s-Hansen and Jensen, {Thomas G.} and Corydon, {Thomas Juhl} and Mikkelsen, {Jacob Giehm} and Lars Aagaard",
year = "2015",
month = sep,
day = "18",
language = "English",
note = "null ; Conference date: 17-09-2015 Through 20-09-2015",

}

RIS

TY - CONF

T1 - Efficient in vivo gene transfer to xenotransplanted human skin by lentivirus-mediated, but not by AAV-directed, gene delivery

AU - Jakobsen, Maria Vad

AU - Askou, Anne Louise

AU - Dokkedahl, Karin Stenderup

AU - Kjeldsen, Cecilia Rosada

AU - Dagnæs-Hansen, Frederik

AU - Jensen, Thomas G.

AU - Corydon, Thomas Juhl

AU - Mikkelsen, Jacob Giehm

AU - Aagaard, Lars

PY - 2015/9/18

Y1 - 2015/9/18

N2 - Skin is an easily accessible organ, and therapeutic gene transfer to skin remains an attractive alternative for treatment of skin diseases. We compared the potential usefulness of various serotypes of recombinant AAV vectors and lentiviral vectors for gene transfer to human skin in a xenotransplanted mouse model. Vector constructs encoding firefly luciferase were packaged in AAV capsids of serotype 1, 2, 5, 6, 8, and 9 and separately administered by intradermal injection in human skin transplants. For all serotypes, live bioimagingdemonstrated low levels of transgene expression in the human skin graft, and firefly luciferase expression was observed primarily in neighboring tissue beneath or surrounding the graft. In contrast, gene delivery by intradermally injected lentiviral vectors was efficient and led to extensive and persistent firefly luciferase expression within the human skin graft only. The study demonstrates limited capacity of single-stranded AAV vectors of six commonly used serotypes for gene delivery to human skin in vivo.

AB - Skin is an easily accessible organ, and therapeutic gene transfer to skin remains an attractive alternative for treatment of skin diseases. We compared the potential usefulness of various serotypes of recombinant AAV vectors and lentiviral vectors for gene transfer to human skin in a xenotransplanted mouse model. Vector constructs encoding firefly luciferase were packaged in AAV capsids of serotype 1, 2, 5, 6, 8, and 9 and separately administered by intradermal injection in human skin transplants. For all serotypes, live bioimagingdemonstrated low levels of transgene expression in the human skin graft, and firefly luciferase expression was observed primarily in neighboring tissue beneath or surrounding the graft. In contrast, gene delivery by intradermally injected lentiviral vectors was efficient and led to extensive and persistent firefly luciferase expression within the human skin graft only. The study demonstrates limited capacity of single-stranded AAV vectors of six commonly used serotypes for gene delivery to human skin in vivo.

UR - http://www.esgct.eu/

M3 - Poster

Y2 - 17 September 2015 through 20 September 2015

ER -