Aarhus University Seal / Aarhus Universitets segl

Effects of mutated pregnancy-associated plasma protein-a on atherosclerotic lesion development in mice

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Effects of mutated pregnancy-associated plasma protein-a on atherosclerotic lesion development in mice. / Boldt, Henning B; Bale, Laurie K; Resch, Zachary T; Oxvig, Claus; Overgaard, Michael Toft; Conover, Cheryl A.

I: Endocrinology, Bind 154, Nr. 1, 01.2013, s. 246-252.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Boldt, HB, Bale, LK, Resch, ZT, Oxvig, C, Overgaard, MT & Conover, CA 2013, 'Effects of mutated pregnancy-associated plasma protein-a on atherosclerotic lesion development in mice', Endocrinology, bind 154, nr. 1, s. 246-252. https://doi.org/10.1210/en.2012-1552

APA

Boldt, H. B., Bale, L. K., Resch, Z. T., Oxvig, C., Overgaard, M. T., & Conover, C. A. (2013). Effects of mutated pregnancy-associated plasma protein-a on atherosclerotic lesion development in mice. Endocrinology, 154(1), 246-252. https://doi.org/10.1210/en.2012-1552

CBE

MLA

Vancouver

Author

Boldt, Henning B ; Bale, Laurie K ; Resch, Zachary T ; Oxvig, Claus ; Overgaard, Michael Toft ; Conover, Cheryl A. / Effects of mutated pregnancy-associated plasma protein-a on atherosclerotic lesion development in mice. I: Endocrinology. 2013 ; Bind 154, Nr. 1. s. 246-252.

Bibtex

@article{4bcdf0c0ef344e218cc9a25390287c45,
title = "Effects of mutated pregnancy-associated plasma protein-a on atherosclerotic lesion development in mice",
abstract = "Pregnancy-associated plasma protein-A (PAPP-A) is a large multidomain metalloprotease involved in cleavage of IGF binding protein (IGFBP)-4 and -5 thereby causing release of bioactive IGF. Individual domains of PAPP-A have been characterized in vitro, including the metzincin proteolytic domain important for IGFBP proteolytic activity, short consensus repeats critical for cell surface association, and Lin-12/Notch repeat module demonstrated to determine IGFBP substrate specificity. To test the hypothesis that specific cleavage of IGFBP-4 by PAPP-A in close proximity to the cell surface is required for development of lesions in a murine model of atherosclerosis, the following PAPP-A transgenic (Tg) mice were generated: Tg(E483A), which lacks all PAPP-A proteolytic activity; Tg(D1499A), which selectively lacks proteolytic activity against IGFBP-4; and Tg(K1296A/K1316A), in which cell surface binding is compromised. Following cross-breeding with apolipoprotein E (ApoE) knockout (KO) mice, ApoE KO/Tg mice were fed a high-fat diet to promote aortic lesion development. Lesion area was increased 2-fold in aortas from ApoE KO/Tg wild-type compared with ApoE KO mice (P",
keywords = "Animals, Aorta, Apolipoproteins E, Atherosclerosis, Cells, Cultured, Enzyme-Linked Immunosorbent Assay, Female, Insulin-Like Growth Factor Binding Protein 4, Male, Mice, Mice, Knockout, Mice, Transgenic, Myocytes, Smooth Muscle, Pregnancy-Associated Plasma Protein-A",
author = "Boldt, {Henning B} and Bale, {Laurie K} and Resch, {Zachary T} and Claus Oxvig and Overgaard, {Michael Toft} and Conover, {Cheryl A.}",
year = "2013",
month = "1",
doi = "10.1210/en.2012-1552",
language = "English",
volume = "154",
pages = "246--252",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "Oxford University Press",
number = "1",

}

RIS

TY - JOUR

T1 - Effects of mutated pregnancy-associated plasma protein-a on atherosclerotic lesion development in mice

AU - Boldt, Henning B

AU - Bale, Laurie K

AU - Resch, Zachary T

AU - Oxvig, Claus

AU - Overgaard, Michael Toft

AU - Conover, Cheryl A.

PY - 2013/1

Y1 - 2013/1

N2 - Pregnancy-associated plasma protein-A (PAPP-A) is a large multidomain metalloprotease involved in cleavage of IGF binding protein (IGFBP)-4 and -5 thereby causing release of bioactive IGF. Individual domains of PAPP-A have been characterized in vitro, including the metzincin proteolytic domain important for IGFBP proteolytic activity, short consensus repeats critical for cell surface association, and Lin-12/Notch repeat module demonstrated to determine IGFBP substrate specificity. To test the hypothesis that specific cleavage of IGFBP-4 by PAPP-A in close proximity to the cell surface is required for development of lesions in a murine model of atherosclerosis, the following PAPP-A transgenic (Tg) mice were generated: Tg(E483A), which lacks all PAPP-A proteolytic activity; Tg(D1499A), which selectively lacks proteolytic activity against IGFBP-4; and Tg(K1296A/K1316A), in which cell surface binding is compromised. Following cross-breeding with apolipoprotein E (ApoE) knockout (KO) mice, ApoE KO/Tg mice were fed a high-fat diet to promote aortic lesion development. Lesion area was increased 2-fold in aortas from ApoE KO/Tg wild-type compared with ApoE KO mice (P

AB - Pregnancy-associated plasma protein-A (PAPP-A) is a large multidomain metalloprotease involved in cleavage of IGF binding protein (IGFBP)-4 and -5 thereby causing release of bioactive IGF. Individual domains of PAPP-A have been characterized in vitro, including the metzincin proteolytic domain important for IGFBP proteolytic activity, short consensus repeats critical for cell surface association, and Lin-12/Notch repeat module demonstrated to determine IGFBP substrate specificity. To test the hypothesis that specific cleavage of IGFBP-4 by PAPP-A in close proximity to the cell surface is required for development of lesions in a murine model of atherosclerosis, the following PAPP-A transgenic (Tg) mice were generated: Tg(E483A), which lacks all PAPP-A proteolytic activity; Tg(D1499A), which selectively lacks proteolytic activity against IGFBP-4; and Tg(K1296A/K1316A), in which cell surface binding is compromised. Following cross-breeding with apolipoprotein E (ApoE) knockout (KO) mice, ApoE KO/Tg mice were fed a high-fat diet to promote aortic lesion development. Lesion area was increased 2-fold in aortas from ApoE KO/Tg wild-type compared with ApoE KO mice (P

KW - Animals

KW - Aorta

KW - Apolipoproteins E

KW - Atherosclerosis

KW - Cells, Cultured

KW - Enzyme-Linked Immunosorbent Assay

KW - Female

KW - Insulin-Like Growth Factor Binding Protein 4

KW - Male

KW - Mice

KW - Mice, Knockout

KW - Mice, Transgenic

KW - Myocytes, Smooth Muscle

KW - Pregnancy-Associated Plasma Protein-A

U2 - 10.1210/en.2012-1552

DO - 10.1210/en.2012-1552

M3 - Journal article

C2 - 23161866

VL - 154

SP - 246

EP - 252

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 1

ER -