TY - JOUR
T1 - Effects of milk extracellular vesicles from bovine, human, and caprine origin on rotavirus infectivity in cultured human intestinal cells
AU - Vangsøe, Caroline
AU - Graikini, Dimitra
AU - Bojsen, Anni
AU - Sánchez, Lourdes
AU - Trige Rasmussen, Jan
N1 - Publisher Copyright:
© 2024 Elsevier Ltd
PY - 2024/7
Y1 - 2024/7
N2 - Rotavirus is the leading cause of severe gastroenteritis in neonates representing a global health burden. Milk-derived extracellular vesicles [EVs] have emerged as potential antivirals, offering a promising avenue for intervention. The present study evaluated the antiviral activity of EVs sourced from bovine, human, and caprine milk against the infection of the human Wa and bovine WC3 rotavirus strains. EVs induced an endogenous effect in Caco-2 cells, resulting in a significant reduction of rotaviral infection. The antiviral properties of the milk EVs, were found to be associated with synergistic interactions at pre- and post-attachment stages of the infection. Finally, the effect of milk processing on bovine milk EVs efficacy was assessed. It was found that after high-temperature short-time pasteurization EVs retained their activity, while homogenization resulted in no antiviral effect at all. Overall, our findings demonstrate a significant reduction in rotavirus infection in cultured Caco-2 cells when treated with milk EVs.
AB - Rotavirus is the leading cause of severe gastroenteritis in neonates representing a global health burden. Milk-derived extracellular vesicles [EVs] have emerged as potential antivirals, offering a promising avenue for intervention. The present study evaluated the antiviral activity of EVs sourced from bovine, human, and caprine milk against the infection of the human Wa and bovine WC3 rotavirus strains. EVs induced an endogenous effect in Caco-2 cells, resulting in a significant reduction of rotaviral infection. The antiviral properties of the milk EVs, were found to be associated with synergistic interactions at pre- and post-attachment stages of the infection. Finally, the effect of milk processing on bovine milk EVs efficacy was assessed. It was found that after high-temperature short-time pasteurization EVs retained their activity, while homogenization resulted in no antiviral effect at all. Overall, our findings demonstrate a significant reduction in rotavirus infection in cultured Caco-2 cells when treated with milk EVs.
UR - http://www.scopus.com/inward/record.url?scp=85189549931&partnerID=8YFLogxK
U2 - 10.1016/j.idairyj.2024.105937
DO - 10.1016/j.idairyj.2024.105937
M3 - Journal article
AN - SCOPUS:85189549931
SN - 0958-6946
VL - 154
JO - International Dairy Journal
JF - International Dairy Journal
M1 - 105937
ER -