Effects of exposure to environmentally relevant concentrations of lead (Pb) on expression of stress and immune-related genes, and microRNAs in shorthorn sculpins (Myoxocephalus scorpius)

Khattapan Jantawongsri; Rasmus Dyrmose Nørregaard; Lis Bach; Rune Dietz; Christian Sonne; Kasper Jørgensen; Syverin Lierhagen; Tomasz Maciej Ciesielski; Bjørn Munro Jenssen; Courtney Alice Waugh; Ruth Eriksen; Barbara Nowak; Kelli Anderson

doi:10.1007/s10646-022-02575-x

Effects of exposure to environmentally relevant concentrations of lead (Pb) on expression of stress and immune-related genes, and microRNAs in shorthorn sculpins (Myoxocephalus scorpius)

Khattapan Jantawongsri^*, Rasmus Dyrmose Nørregaard, Lis Bach, Rune Dietz, Christian Sonne, Kasper Jørgensen, Syverin Lierhagen, Tomasz Maciej Ciesielski, Bjørn Munro Jenssen, Courtney Alice Waugh, Ruth Eriksen, Barbara Nowak, Kelli Anderson

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

2 Citationer (Scopus)

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Abstract

Abstract: Old lead–zinc (Pb–Zn) mining sites in Greenland have increased the environmental concentration of Pb in local marine organisms, including the shorthorn sculpin. Organ metal concentrations and histopathology have been used in environmental monitoring programs to evaluate metal exposure and subsequent effects in shorthorn sculpins. So far, no study has reported the impact of heavy metals on gene expression involved in metal-related stress and immune responses in sculpins. The aim of this study was to investigate the effect of exposure to environmentally relevant waterborne Pb (0.73 ± 0.35 μg/L) on hepatic gene expression of metallothionein (mt), immunoglobulin M (igm), and microRNAs (miRNAs; mir132 and mir155) associated with immune responses in the shorthorn sculpin compared to a control group. The mt and igm expression were upregulated in the Pb-exposed group compared to the control group. The transcripts of mir132 and mir155 were not different in sculpins between the Pb-exposed and control group; however, miRNA levels were significantly correlated with Pb liver concentrations. Furthermore, there was a positive correlation between liver Pb concentrations and igm, and a positive relationship between igm and mir155. The results indicate that exposure to Pb similar to those concentrations reported in in marine waters around Greenland Pb–Zn mine sites influences the mt and immune responses in shorthorn sculpins. This is the first study to identify candidate molecular markers in the shorthorn sculpins exposed to waterborne environmentally relevant Pb suggesting mt and igm as potential molecular markers of exposure to be applied in future assessments of the marine environment near Arctic mining sites.

Originalsprog	Engelsk
Tidsskrift	Ecotoxicology
Vol/bind	31
Nummer	7
Sider (fra-til)	1068-1077
Antal sider	10
ISSN	0963-9292
DOI	https://doi.org/10.1007/s10646-022-02575-x
Status	Udgivet - sep. 2022

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Jantawongsri, K., Nørregaard, R. D., Bach, L., Dietz, R., Sonne, C., Jørgensen, K., Lierhagen, S., Ciesielski, T. M., Jenssen, B. M., Waugh, C. A., Eriksen, R., Nowak, B., & Anderson, K. (2022). Effects of exposure to environmentally relevant concentrations of lead (Pb) on expression of stress and immune-related genes, and microRNAs in shorthorn sculpins (Myoxocephalus scorpius). Ecotoxicology, 31(7), 1068-1077. https://doi.org/10.1007/s10646-022-02575-x

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title = "Effects of exposure to environmentally relevant concentrations of lead (Pb) on expression of stress and immune-related genes, and microRNAs in shorthorn sculpins (Myoxocephalus scorpius)",

abstract = "Abstract: Old lead–zinc (Pb–Zn) mining sites in Greenland have increased the environmental concentration of Pb in local marine organisms, including the shorthorn sculpin. Organ metal concentrations and histopathology have been used in environmental monitoring programs to evaluate metal exposure and subsequent effects in shorthorn sculpins. So far, no study has reported the impact of heavy metals on gene expression involved in metal-related stress and immune responses in sculpins. The aim of this study was to investigate the effect of exposure to environmentally relevant waterborne Pb (0.73 ± 0.35 μg/L) on hepatic gene expression of metallothionein (mt), immunoglobulin M (igm), and microRNAs (miRNAs; mir132 and mir155) associated with immune responses in the shorthorn sculpin compared to a control group. The mt and igm expression were upregulated in the Pb-exposed group compared to the control group. The transcripts of mir132 and mir155 were not different in sculpins between the Pb-exposed and control group; however, miRNA levels were significantly correlated with Pb liver concentrations. Furthermore, there was a positive correlation between liver Pb concentrations and igm, and a positive relationship between igm and mir155. The results indicate that exposure to Pb similar to those concentrations reported in in marine waters around Greenland Pb–Zn mine sites influences the mt and immune responses in shorthorn sculpins. This is the first study to identify candidate molecular markers in the shorthorn sculpins exposed to waterborne environmentally relevant Pb suggesting mt and igm as potential molecular markers of exposure to be applied in future assessments of the marine environment near Arctic mining sites.",

keywords = "Arctic lead–zinc mines, Dissolved Pb exposure, Gene expression, Greenland sculpin, Immune-related gene, Metal stress-related gene",

author = "Khattapan Jantawongsri and N{\o}rregaard, {Rasmus Dyrmose} and Lis Bach and Rune Dietz and Christian Sonne and Kasper J{\o}rgensen and Syverin Lierhagen and Ciesielski, {Tomasz Maciej} and Jenssen, {Bj{\o}rn Munro} and Waugh, {Courtney Alice} and Ruth Eriksen and Barbara Nowak and Kelli Anderson",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

month = sep,

doi = "10.1007/s10646-022-02575-x",

language = "English",

volume = "31",

pages = "1068--1077",

journal = "Ecotoxicology",

issn = "0963-9292",

publisher = "Springer",

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Jantawongsri, K, Nørregaard, RD, Bach, L , Dietz, R , Sonne, C, Jørgensen, K, Lierhagen, S, Ciesielski, TM, Jenssen, BM, Waugh, CA, Eriksen, R, Nowak, B & Anderson, K 2022, 'Effects of exposure to environmentally relevant concentrations of lead (Pb) on expression of stress and immune-related genes, and microRNAs in shorthorn sculpins (Myoxocephalus scorpius)', Ecotoxicology, bind 31, nr. 7, s. 1068-1077. https://doi.org/10.1007/s10646-022-02575-x

Effects of exposure to environmentally relevant concentrations of lead (Pb) on expression of stress and immune-related genes, and microRNAs in shorthorn sculpins (Myoxocephalus scorpius). / Jantawongsri, Khattapan; Nørregaard, Rasmus Dyrmose; Bach, Lis et al.
I: Ecotoxicology, Bind 31, Nr. 7, 09.2022, s. 1068-1077.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - Effects of exposure to environmentally relevant concentrations of lead (Pb) on expression of stress and immune-related genes, and microRNAs in shorthorn sculpins (Myoxocephalus scorpius)

AU - Jantawongsri, Khattapan

AU - Nørregaard, Rasmus Dyrmose

AU - Bach, Lis

AU - Dietz, Rune

AU - Sonne, Christian

AU - Jørgensen, Kasper

AU - Lierhagen, Syverin

AU - Ciesielski, Tomasz Maciej

AU - Jenssen, Bjørn Munro

AU - Waugh, Courtney Alice

AU - Eriksen, Ruth

AU - Nowak, Barbara

AU - Anderson, Kelli

PY - 2022/9

Y1 - 2022/9

N2 - Abstract: Old lead–zinc (Pb–Zn) mining sites in Greenland have increased the environmental concentration of Pb in local marine organisms, including the shorthorn sculpin. Organ metal concentrations and histopathology have been used in environmental monitoring programs to evaluate metal exposure and subsequent effects in shorthorn sculpins. So far, no study has reported the impact of heavy metals on gene expression involved in metal-related stress and immune responses in sculpins. The aim of this study was to investigate the effect of exposure to environmentally relevant waterborne Pb (0.73 ± 0.35 μg/L) on hepatic gene expression of metallothionein (mt), immunoglobulin M (igm), and microRNAs (miRNAs; mir132 and mir155) associated with immune responses in the shorthorn sculpin compared to a control group. The mt and igm expression were upregulated in the Pb-exposed group compared to the control group. The transcripts of mir132 and mir155 were not different in sculpins between the Pb-exposed and control group; however, miRNA levels were significantly correlated with Pb liver concentrations. Furthermore, there was a positive correlation between liver Pb concentrations and igm, and a positive relationship between igm and mir155. The results indicate that exposure to Pb similar to those concentrations reported in in marine waters around Greenland Pb–Zn mine sites influences the mt and immune responses in shorthorn sculpins. This is the first study to identify candidate molecular markers in the shorthorn sculpins exposed to waterborne environmentally relevant Pb suggesting mt and igm as potential molecular markers of exposure to be applied in future assessments of the marine environment near Arctic mining sites.

AB - Abstract: Old lead–zinc (Pb–Zn) mining sites in Greenland have increased the environmental concentration of Pb in local marine organisms, including the shorthorn sculpin. Organ metal concentrations and histopathology have been used in environmental monitoring programs to evaluate metal exposure and subsequent effects in shorthorn sculpins. So far, no study has reported the impact of heavy metals on gene expression involved in metal-related stress and immune responses in sculpins. The aim of this study was to investigate the effect of exposure to environmentally relevant waterborne Pb (0.73 ± 0.35 μg/L) on hepatic gene expression of metallothionein (mt), immunoglobulin M (igm), and microRNAs (miRNAs; mir132 and mir155) associated with immune responses in the shorthorn sculpin compared to a control group. The mt and igm expression were upregulated in the Pb-exposed group compared to the control group. The transcripts of mir132 and mir155 were not different in sculpins between the Pb-exposed and control group; however, miRNA levels were significantly correlated with Pb liver concentrations. Furthermore, there was a positive correlation between liver Pb concentrations and igm, and a positive relationship between igm and mir155. The results indicate that exposure to Pb similar to those concentrations reported in in marine waters around Greenland Pb–Zn mine sites influences the mt and immune responses in shorthorn sculpins. This is the first study to identify candidate molecular markers in the shorthorn sculpins exposed to waterborne environmentally relevant Pb suggesting mt and igm as potential molecular markers of exposure to be applied in future assessments of the marine environment near Arctic mining sites.

KW - Arctic lead–zinc mines

KW - Dissolved Pb exposure

KW - Gene expression

KW - Greenland sculpin

KW - Immune-related gene

KW - Metal stress-related gene

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U2 - 10.1007/s10646-022-02575-x

DO - 10.1007/s10646-022-02575-x

M3 - Journal article

C2 - 36006498

AN - SCOPUS:85137253404

SN - 0963-9292

VL - 31

SP - 1068

EP - 1077

JO - Ecotoxicology

JF - Ecotoxicology

IS - 7

ER -

Effects of exposure to environmentally relevant concentrations of lead (Pb) on expression of stress and immune-related genes, and microRNAs in shorthorn sculpins (Myoxocephalus scorpius)

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