Effects of dietary tryptophan and threonine supplementation above nutritional recommendations on performance, diarrhoea, and intestinal health parameters in weaned piglets

Maiken Christina N Engelsmann, Tina Skau Nielsen, Mette Skou Hedemann, Uffe Krogh, Jan Værum Nørgaard*

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Abstract

Pigs may have an enhanced need for the amino acids (AA) tryptophan (Trp) and threonine (Thr) around weaning.
This study aimed to evaluate the effect of additional dietary Trp and Thr above nutrient requirements; on performance,
the probability of diarrhoea, intestinal permeability, and morphology in weaned pigs. A total of 120
piglets (7.20 ± 0.26 kg), weaned at day 28, were randomly allocated to five dietary treatments and individually
housed. A standard diet with maximum crude protein (CP) according to Danish nutrient recommendations was
used as control diet (HP). The other dietary treatments consisted of the HP diet with additional 20% Thr (THR),
20% Trp (TRP) or 20% of both (THR+TRP) relative to the recommendations for these AA (standardised ileal
digestible (SID); 7.8 g SID Thr/kg and 2.6 g SID Trp/kg). Finally, a low-protein diet according to recommendations
was included as a negative control (203 g CP/kg DM; LP). Diets were designed to be isoenergetic and fed
ad libitum from 0 to 4 weeks post-weaning. Faecal consistency was evaluated daily using a 4-scale visual scoring
system. Blood was collected on day 4, 14, 21, and 28 and small intestinal and colonic tissue were obtained from
HP, LP, and THR+THR pigs at day 28. Average daily gain and feed intake were unaffected by diet (P > 0.10),
whereas the feed conversion ratio was increased with 11% in LP compared with HP pigs (P < 0.05). Additionally,
Thr and Trp did not affect the probability for diarrhoea (P > 0.10), neither in separate weeks nor across the entire
period. The number of antibiotic treatment days were 3.92 days higher for TRP compared with THR+TRP pigs
(P = 0.01), with the other three diets being intermediate. The concentration of the intestinal permeability
markers diamine oxidase and D-lactate in blood was unaffected by the diets. The systemic inflammatory markers
haptoglobin and C-reactive protein did not differ between feeding groups. The THR+TRP diet increased the area
of neutral mucin-producing cells in the colon with 83% compared with the HP diet (P = 0.02), but other intestinal
morphology measurements were unaffected by the diets. In conclusion, supplementation with Thr and
Trp together stimulated neutral mucin production in the large intestine and reduced the number of days where
antibiotics treatments against diarrhoea is necessary.
OriginalsprogEngelsk
Artikelnummer105186
TidsskriftLivestock Science
Vol/bind269
Antal sider10
ISSN1871-1413
DOI
StatusUdgivet - mar. 2023

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