Aarhus University Seal / Aarhus Universitets segl

Effect of voluntary exercise on number and volume of cardiomyocytes and their mitochondria in the mouse left ventricle

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Effect of voluntary exercise on number and volume of cardiomyocytes and their mitochondria in the mouse left ventricle. / Eisele, Judith Christina; Schaefer, Inga-Marie; Randel Nyengaard, Jens; Post, Heiner; Liebetanz, David; Brüel, Annemarie; Mühlfeld, Christian.

I: Basic Research in Cardiology, Bind 103, Nr. 1, 2008, s. 12-21.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Eisele, JC, Schaefer, I-M, Randel Nyengaard, J, Post, H, Liebetanz, D, Brüel, A & Mühlfeld, C 2008, 'Effect of voluntary exercise on number and volume of cardiomyocytes and their mitochondria in the mouse left ventricle', Basic Research in Cardiology, bind 103, nr. 1, s. 12-21. https://doi.org/10.1007/s00395-007-0684-x

APA

CBE

MLA

Vancouver

Author

Eisele, Judith Christina ; Schaefer, Inga-Marie ; Randel Nyengaard, Jens ; Post, Heiner ; Liebetanz, David ; Brüel, Annemarie ; Mühlfeld, Christian. / Effect of voluntary exercise on number and volume of cardiomyocytes and their mitochondria in the mouse left ventricle. I: Basic Research in Cardiology. 2008 ; Bind 103, Nr. 1. s. 12-21.

Bibtex

@article{37ef1970fcf011dda987000ea68e967b,
title = "Effect of voluntary exercise on number and volume of cardiomyocytes and their mitochondria in the mouse left ventricle",
abstract = "Voluntary exercise (VE) has a beneficial influence on the heart and mean lifespan. The present study evaluates structural adaptations of cardiomyocytes and their mitochondria due to VE by new, unbiased stereological methods. Female, 7-9-week-old mice were randomly assigned to a control (CG, n = 7) or VE group (EG, n = 7). EG animals were housed in cages with free access to a running wheel and had a mean running distance of 6.7 (1.8) km per day. After 4 weeks, the hearts of all mice were processed for light and electron microscopy. We estimated the number and volume of cardiomyocytes by the disector method and the number and volume of mitochondria by estimation of the Euler number. In comparison to CG, VE did not have an effect on the myocardial volume of the left ventricle (CG: 93 (10), EG: 103 (17) (mm(3))), the number of cardiomyocytes (CG: 2.81 (0.27), EG: 2.82 (0.43) (x10(6))) and their number-weighted mean volume. However, the composition of the cardiomyocytes changed due to VE. The total volume of mitochondria (CG: 21.8 (4.9), EG: 32.2 (4.3) (mm(3)), P < 0.01) and the total number (CG: 3.76 (0.44), EG: 7.02 (1.13) (x10(10)), P < 0.001) were significantly higher in EG than in CG. The mean number-weighted mitochondrial volume was smaller in EG than in CG (P < 0.05). In summary, VE does not alter ventricular volume nor cardiomyocyte volume or number but the oxidative capacity of cardiomyocytes by an increased mitochondrial number and total volume in the left ventricle. These structural changes may participate in the beneficial effects of VE.",
keywords = "Animals, Female, Heart Ventricles, Mice, Mice, Inbred C57BL, Microscopy, Electron, Mitochondria, Heart, Myocytes, Cardiac, Organ Size, Physical Conditioning, Animal, Random Allocation, Running, Statistics as Topic, Ventricular Function, Volition",
author = "Eisele, {Judith Christina} and Inga-Marie Schaefer and {Randel Nyengaard}, Jens and Heiner Post and David Liebetanz and Annemarie Br{\"u}el and Christian M{\"u}hlfeld",
year = "2008",
doi = "10.1007/s00395-007-0684-x",
language = "English",
volume = "103",
pages = "12--21",
journal = "Basic Research in Cardiology",
issn = "0300-8428",
publisher = "Springer Medizin",
number = "1",

}

RIS

TY - JOUR

T1 - Effect of voluntary exercise on number and volume of cardiomyocytes and their mitochondria in the mouse left ventricle

AU - Eisele, Judith Christina

AU - Schaefer, Inga-Marie

AU - Randel Nyengaard, Jens

AU - Post, Heiner

AU - Liebetanz, David

AU - Brüel, Annemarie

AU - Mühlfeld, Christian

PY - 2008

Y1 - 2008

N2 - Voluntary exercise (VE) has a beneficial influence on the heart and mean lifespan. The present study evaluates structural adaptations of cardiomyocytes and their mitochondria due to VE by new, unbiased stereological methods. Female, 7-9-week-old mice were randomly assigned to a control (CG, n = 7) or VE group (EG, n = 7). EG animals were housed in cages with free access to a running wheel and had a mean running distance of 6.7 (1.8) km per day. After 4 weeks, the hearts of all mice were processed for light and electron microscopy. We estimated the number and volume of cardiomyocytes by the disector method and the number and volume of mitochondria by estimation of the Euler number. In comparison to CG, VE did not have an effect on the myocardial volume of the left ventricle (CG: 93 (10), EG: 103 (17) (mm(3))), the number of cardiomyocytes (CG: 2.81 (0.27), EG: 2.82 (0.43) (x10(6))) and their number-weighted mean volume. However, the composition of the cardiomyocytes changed due to VE. The total volume of mitochondria (CG: 21.8 (4.9), EG: 32.2 (4.3) (mm(3)), P < 0.01) and the total number (CG: 3.76 (0.44), EG: 7.02 (1.13) (x10(10)), P < 0.001) were significantly higher in EG than in CG. The mean number-weighted mitochondrial volume was smaller in EG than in CG (P < 0.05). In summary, VE does not alter ventricular volume nor cardiomyocyte volume or number but the oxidative capacity of cardiomyocytes by an increased mitochondrial number and total volume in the left ventricle. These structural changes may participate in the beneficial effects of VE.

AB - Voluntary exercise (VE) has a beneficial influence on the heart and mean lifespan. The present study evaluates structural adaptations of cardiomyocytes and their mitochondria due to VE by new, unbiased stereological methods. Female, 7-9-week-old mice were randomly assigned to a control (CG, n = 7) or VE group (EG, n = 7). EG animals were housed in cages with free access to a running wheel and had a mean running distance of 6.7 (1.8) km per day. After 4 weeks, the hearts of all mice were processed for light and electron microscopy. We estimated the number and volume of cardiomyocytes by the disector method and the number and volume of mitochondria by estimation of the Euler number. In comparison to CG, VE did not have an effect on the myocardial volume of the left ventricle (CG: 93 (10), EG: 103 (17) (mm(3))), the number of cardiomyocytes (CG: 2.81 (0.27), EG: 2.82 (0.43) (x10(6))) and their number-weighted mean volume. However, the composition of the cardiomyocytes changed due to VE. The total volume of mitochondria (CG: 21.8 (4.9), EG: 32.2 (4.3) (mm(3)), P < 0.01) and the total number (CG: 3.76 (0.44), EG: 7.02 (1.13) (x10(10)), P < 0.001) were significantly higher in EG than in CG. The mean number-weighted mitochondrial volume was smaller in EG than in CG (P < 0.05). In summary, VE does not alter ventricular volume nor cardiomyocyte volume or number but the oxidative capacity of cardiomyocytes by an increased mitochondrial number and total volume in the left ventricle. These structural changes may participate in the beneficial effects of VE.

KW - Animals

KW - Female

KW - Heart Ventricles

KW - Mice

KW - Mice, Inbred C57BL

KW - Microscopy, Electron

KW - Mitochondria, Heart

KW - Myocytes, Cardiac

KW - Organ Size

KW - Physical Conditioning, Animal

KW - Random Allocation

KW - Running

KW - Statistics as Topic

KW - Ventricular Function

KW - Volition

U2 - 10.1007/s00395-007-0684-x

DO - 10.1007/s00395-007-0684-x

M3 - Journal article

C2 - 18004633

VL - 103

SP - 12

EP - 21

JO - Basic Research in Cardiology

JF - Basic Research in Cardiology

SN - 0300-8428

IS - 1

ER -